Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Taxol-carried nano microbubble and preparation method thereof

A paclitaxel and microbubble technology, which is applied in the field of medical drugs, can solve the problems of patients' intolerance, high toxicity and side effects, etc., and achieve the effect of long duration, good stability, and widening of the endothelial cell gap

Inactive Publication Date: 2012-02-22
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the dissolution problem of paclitaxel has been solved, it is difficult for patients to tolerate due to the large toxic and side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Taxol-carried nano microbubble and preparation method thereof
  • Taxol-carried nano microbubble and preparation method thereof
  • Taxol-carried nano microbubble and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A paclitaxel-loaded nano-microbubble, the microbubble consists of a lipid bilayer membrane, containing N 2 , the lipid bilayer comprises a hydrophilic group and a hydrophobic group, the hydrophilic group links paclitaxel, and paclitaxel is loaded into the membrane formed by the lipid bilayer, and the lipid bimolecular The layer is composed of lecithin, cholesterol, PEG-4000, dextran, Span-80, methanol, ether, 3% HSA, paclitaxel standard substance, and PBS liquid. Its structure is as follows: figure 1 shown.

[0059] The preparation method of paclitaxel-loaded nano-microbubbles is carried out as follows:

[0060] (1) Put 0.1g lecithin and 0.04g cholesterol into the reaction bottle of the rotary evaporator, add ether to dissolve the lecithin and cholesterol; 40 ℃ water bath, the rotary evaporator works, see the bottom of the reaction bottle forms a layer of dry film to end the reaction , recover the ether into a bottle and keep it properly;

[0061] (2) configure 0.01...

Embodiment 2

[0067] A paclitaxel-loaded nano-microbubble, the microbubble consists of a lipid bilayer membrane, containing N 2 , the lipid bilayer comprises a hydrophilic group and a hydrophobic group, the hydrophilic group links paclitaxel, and paclitaxel is loaded into the membrane formed by the lipid bilayer, and the lipid bimolecular The layer is composed of lecithin, cholesterol, PEG-4000, dextran, Span-80, methanol, ether, 3% HSA, paclitaxel standard substance, and PBS liquid. Its structure is as follows: figure 1 shown.

[0068] The preparation method of paclitaxel-loaded nano-microbubbles is carried out as follows:

[0069] (1) Put 0.15g lecithin and 0.06g cholesterol into the reaction bottle of the rotary evaporator, add ether to dissolve the lecithin and cholesterol; 40 ℃ water bath, the rotary evaporator works, see the bottom of the reaction bottle forms a layer of dry film to end the reaction , recover the ether into a bottle and keep it properly;

[0070] (2) Configure 0.0...

Embodiment 3

[0076] A paclitaxel-loaded nano-microbubble, the microbubble consists of a lipid bilayer membrane, containing N 2 , the lipid bilayer comprises a hydrophilic group and a hydrophobic group, the hydrophilic group links paclitaxel, and paclitaxel is loaded into the membrane formed by the lipid bilayer, and the lipid bimolecular The layer is composed of lecithin, cholesterol, PEG-4000, dextran, Span-80, methanol, ether, 3% HSA, paclitaxel standard substance, and PBS liquid. Its structure is as follows: figure 1 shown.

[0077] The preparation method of paclitaxel-loaded nano-microbubbles is carried out as follows:

[0078] (1) Put 0.12g lecithin and 0.05g cholesterol into the reaction bottle of the rotary evaporator, add ether to dissolve the lecithin and cholesterol; 40°C water bath, the rotary evaporator works, see the bottom of the reaction bottle forms a layer of dry film to end the reaction , recover the ether into a bottle and keep it properly;

[0079] (2) Configure 0.0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a taxol-carried nano microbubble and a preparation method thereof. The microbubble has a membrane consisting of a lipoid bilayer and containing N2; the lipoid bilayer comprises hydrophilic groups and hydrophobic groups; the hydrophilic groups are bonded with taxol and the taxol is carried in the membrane consisting of the lipoid bilayer; and the lipoid bilayer is formed by compounding lecithin, cholesterol, polyethylene glycol (PEG)-4000, glucan, Span 80, methanol, ether, 3 percent human serum albumin, a taxol standard substance, and phosphate buffered saline (PBS) liquid. The taxol-carried nano microbubble does not have toxicity or side effect, can be used for peripheral intravenous injection, can pass through the lung and a capillary bed of the whole body (the diameter is generally less than 5 um), can be circulated to tissues of the whole body along with blood after the intravenous injection, has high stability, has a long duration in blood circulation, anddoes not influence blood flow dynamic of the whole body or a certain system.

Description

technical field [0001] The invention belongs to the field of medical medicine, and in particular relates to a paclitaxel-loaded nano-microbubble and a preparation method thereof. Background technique [0002] Restenosis often occurs after congenital or acquired vascular stenosis is treated by interventional surgery. The main pathological basis is the neointimal hyperplasia caused by the proliferation and migration of vascular smooth muscle cells. Paclitaxel, a microtubule inhibitor, has a stabilizing effect on microtubule dynamics, inhibits the normal formation of the spindle body, and inhibits transmembrane signal transduction and gene expression related to cell division, so its effect of blocking cell mitosis and proliferation is definite. Experiments have shown that paclitaxel can inhibit the division and proliferation of VSMCs, and can significantly reduce neointimal hyperplasia in restenosis models. [0003] Paclitaxel is white or off-white powder, highly fat-soluble,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/00A61K9/10A61K31/337A61P35/00
Inventor 计晓娟杨汝铃唐静
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com