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Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)

A rapid determination of Camellia oleifera seed technology, applied in the field of edible vegetable oil quality analysis, to achieve the effect of good application value

Inactive Publication Date: 2012-02-15
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic and foreign studies on NIRS analysis of the oil content of camellia oleifera seeds have never been reported.

Method used

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  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)
  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)
  • Method for rapid determination of oil content in Camellia oleifera seeds by using near-infrared diffuse reflectance spectroscopy (NIRS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for quickly measuring the oil content of camellia oleifera seeds by near-infrared diffuse reflectance spectroscopy (NIRS), is characterized in that the near-infrared diffuse reflectance spectroscopy analysis technology measures the content of oil in different varieties of camellia oleifera seeds, and consists of the following steps:

[0041] The first step is the selection of high-quality Camellia oleifera varieties

[0042] Select high-quality varieties from 120-150 varieties of Camellia oleifera seeds, with oleic acid content of 65%-85%, preferably 70%-80%, linoleic acid content of 5%-20%, preferably 7%-12%, palmitic acid content 3%-15%, preferably 7%-11%, stearic acid content 1%-5%, preferably 1%-3%;

[0043] The second step is the basic data determination of camellia seed samples

[0044] The oil content of camellia oleifera seed oil was determined by Soxhlet extraction method, and the fatty acid content in camellia oil was analyzed by gas chromatography. ...

Embodiment 2

[0054] The determination of the oil content of different varieties of Camellia oleifera seeds in embodiment 2

[0055] Grind the dried Camellia oleifera seeds into powder (with seed husks, the water content of the sample is less than 10%), weigh a certain amount of Camellia oleifera seed powder, and record the weight m 0 . Put it into the filter paper tube, insert the absorbent cotton into the upper part, compress the sample, and put it into the extractor. Add 180mL of petroleum ether (60°C-90°C) into the extraction bottle, extract at 80°C for 6h, evaporate the extract to empty and concentrate the solvent to obtain camellia oil, weigh the oil in m 1 . Calculate the oil content w=m of the corresponding variety 1 / m 0 . The oil content of 130 varieties of camellia seeds are shown in Table 3 (samples No. 1 to No. 120 are from Jiangxi).

[0056] Camellia oleifera seed samples of different varieties in Yunnan, Jiangxi, Zhejiang and other places were selected in this embodimen...

Embodiment 3

[0057] The composition of fatty acid in the different kinds of camellia oils measured by gas chromatography in embodiment 3

[0058]The varieties selected in this example include Guangxi (Lingyun, Liuzhou Rongshui, Fangchengdongzhong, Baise, Fengshan, Rong'an, Tianyang, Nandan, common Camellia oleifera), white camellia oil, safflower camellia oil, Yunnan Funingyan A small amount of 129 samples (as shown in Table 3), including 1-120 samples from North Korea and Jiangxi, were dropped into a 10mL test tube, and 2mL of 0.5mol / L NaOH-CH 3 OH, shake well, put it in a 60°C water bath for methyl esterification for 30min, take it out and add 5mL of n-hexane, shake well, take out the supernatant after standing still, and carry out GC chromatographic analysis.

[0059] GC analysis conditions: chromatographic column SE-30; hydrogen flame ionization detector (FID); inlet temperature 280°C, detector temperature 280°C, column temperature 200°C, last 5min, and heat up at a rate of 5°C per min...

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Abstract

The invention discloses a method for rapid determination of oil content in Camellia oleifera seeds by using NIRS. According to the invention, a high-quality Camellia oleifera seed variety is selected from over 130 Camellia oleifera seed varieties, and the selected Camellia oleifera seed variety contains 70% to 80% of tea oil oleic acid, 7% to 12% of linoleic acid, 7% to 11% of palmitic acid and 1% to 3% of stearic acid; chemical values of oil content in selected Camellia oleifera seeds are analyzed, and a precise analysis model is established by combining the chemical values with NIRS; combined pretreatment methods of first derivative, SG (Savitzky-Golay filter) and MSC (multiplicative signal correction) produce a best effect, optimal wave bands are 4200.20 to 4088.35 / cm and 4666.89-4639.89.89 / cm, and an optimal regression method is the method of partial least squares; oil content in Camellia oleifera seed samples to be determined can be obtained by analyzing near-infrared spectrogram of the Camellia oleifera seed samples to be determined by using an established correction model, and a correlation coefficient between analytical values and predicted values can reach 0.8978. The method provided in the invention can be used for rapid and accurate determination of oil content in unknown Camellia oleifera seeds and has a good application value in high-quality breeding and quality analysis of Camellia oleifera.

Description

technical field [0001] The invention relates to the field of edible vegetable oil quality analysis, in particular to a method for quickly measuring the oil content of camellia oleifera seeds by near-infrared diffuse reflectance spectroscopy (NIRS). Background technique [0002] Camellia oleifera (Camellia oleifera), an evergreen small tree in the family Theaceae, is a unique resource in my country. It is as famous as oil palm, olive and coconut as the world's four largest woody edible oil source tree species. Camellia oleifera is mainly distributed in more than ten provinces such as Hunan, Jiangxi, Guangxi, and Zhejiang in my country. The unsaturated fatty acid content in camellia oil is as high as 93%, of which oleic acid is 74% to 87%, linoleic acid is 7% to 14%, and its monounsaturated fatty acid content is the highest among edible oils at present. In addition, tea oil is also rich in vitamin E, vitamin D, carotene, phospholipids, etc. Long-term consumption of tea oil is...

Claims

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Application Information

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IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 王成章原姣姣陈虹霞周昊叶建中
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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