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Preparation method of bionic coating carrying bioactive factors

A bioactive factor and coating technology, applied in coatings, dental preparations, pharmaceutical formulations, etc., can solve problems such as low efficiency, poor stability, and small binding force, and achieve the effect of achieving osseointegration and promoting early occurrence

Inactive Publication Date: 2012-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former introduces uneven coating distribution, low efficiency, poor stability, poor repeatability, and low bonding force, which is easily wiped off during implant placement
The latter is likely to cause the conformation of biologically active factors and affect their biological activity

Method used

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  • Preparation method of bionic coating carrying bioactive factors
  • Preparation method of bionic coating carrying bioactive factors
  • Preparation method of bionic coating carrying bioactive factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of RGD-crosslinked collagen / hyaluronic acid polyelectrolyte composite layer loaded with bioactive factors

[0040] Dissolve the RGD-containing polypeptide segment GRGDSPC (S-S) CPSDGRG and a certain amount of hyaluronic acid in water, then add EDC / NHS, react for 4 hours, the reaction product is dialyzed for 1 week, and freeze-dried; the reaction product is directly dissolved in water, added Muthulose was reacted for 4 hours, dialyzed for one week, and freeze-dried to obtain RGD-grafted sodium hyaluronate with free sulfhydryl groups.

[0041] The RGD-grafted hyaluronic acid and collagen were dissolved in 0.1 mol / L NaCl solution, and the concentration of both was 1 mg / ml. Add BMP-2 to the prepared collagen solution so that the final concentration in the solution is 20 μg / ml (if bFGF is added, the final concentration is 0.1 mg / ml). The pH value of the prepared above solution was adjusted to 4.0. The surface of the implant was sandblasted with large...

Embodiment 2

[0042] Example 2: Preparation of acid-soluble gelatin / hyaluronic acid polyelectrolyte composite layer loaded with bioactive factors cross-linked by RGD

[0043] The method is the same as in Example 1, except that the collagen is replaced with acid-soluble gelatin (the source of which is porcine, bovine or mouse).

Embodiment 3

[0044] Example 3: Preparation of RGD crosslinked chitosan / hyaluronic acid polyelectrolyte composite layer loaded with biologically active factors

[0045] The method is the same as in Example 1, except that the collagen is replaced by chitosan.

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Abstract

The invention provides a preparation method of bionic coating carrying bioactive factors, which is characterized in that: under a catalyzing system of carbodiimide and N-hydroxy-succinamide, Arg-Gly-Asp (RGD) polypeptide chain containing a disulfide bond is grafted onto polyelectrolyte and then is prepared into RGD grafted polyanion electrolyte solution and RGD grafted bioactive-factor-loaded polycation electrolyte solution, the polyanion electrolyte solution is combined with the polycation electrolyte solution carrying the bioactive factors to establish an organic composite coating carrying the bioactive factors on the surface of planting body through a static self-assembling technology. The composite coating solves the bottleneck problem of bioactive factors used surrounding the planting body, can promote the early growth of osteogenesis surrounding the planting body so as to realize the early osseointegration, and facilitates the long-term stability of the planting body. The method is simple to operate, has moderate preparation conditions, is convenient to control and can realize the continuous production.

Description

technical field [0001] The invention belongs to the technical field of medical material manufacturing, and relates to a method for preparing a bionic coating loaded with bioactive factors, in particular to a method for preparing a bionic coating with a slow-release function of bioactive factors. sexual treatment. The biomimetic coating constructed on the surface of the implant by this method simulates the tissue form of the natural extracellular matrix. It not only has biological macromolecules constituting its main structure but also loads bioactive factors that can regulate cell behavior. The slow-release effect of one or more bioactive factors can promote rapid osseointegration between the implant and bone tissue, and is also beneficial to the long-term stability of the implant. Background technique [0002] The theory of osseointegration proposed by Professor Branemark is the core theory in the field of oral implantology. The osseointegration of the implant and the sur...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54A61K6/04
Inventor 李晓东黄颖李晓军
Owner ZHEJIANG UNIV
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