Method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501

Abstract

The invention relates to a method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501. The method comprises the following steps of removing fat-like substances on a cell membrane through a degreasing process, extracting teichoic acid to improve cell membrane permeability, removing protein-like substances in cells through an enzymolysis solution, carrying out centrifugation cleaning to remove impurities, and carrying out freeze-drying to obtain whole peptidoglycan. The whole peptidoglycan extracted by the method has a complete cell wall structure and has good antitumor effects. The method has the advantages of strong practicality, simple operation, short period, simple and reasonable processes, low cost, high yield, and good adaptability for large-scale production. The invention also relates to whole peptidoglycan prepared by the method and a medicinal preparation prepared from the whole peptidoglycan.

Description

technical field [0001] The invention relates to a method for extracting complete peptidoglycan from bifidobacterium longum NQ-1501, in particular to a method for applying a chemical method to extract complete peptidoglycan from probiotics. Background technique [0002] Cancer is one of the important diseases that threaten human life. The World Health Organization and the health departments of governments all regard conquering cancer as an important task. The research and development of new and efficient anti-tumor drugs not only has good economic benefits, but also brings huge social benefits. In general, anticancer drugs are very expensive due to the scarcity of raw materials or high technological content. Therefore, research and production of an anticancer drug with significant curative effect and low price is needed by society. [0003] Scholars at home and abroad have clearly clarified that Bifidobacterium longum is a physiologically beneficial bacterium, which plays a...

AI Technical Summary

Problems solved by technology

For example, "Research on the Separation and Identification of the Complete Peptidoglycan from the Cell Wall of Bifidobacterium longum", "Food and Fermentation Industry", compared with the above-mentioned foreign technology, it has been greatly improved, but the entire production cycle is still relatively lengthy. In particular, the enzymatic hydrolysis step needs to take more than 48 hours, which accounts for most of the time of the whole preparation process, which is not conducive to large-scale process production and product quality control, and needs to remove TCA with methanol. The solvent usage is large and the cost is high. high

[0011] Another example, the patent application "2009102538723, a complete peptidoglycan and its preparation method" is also an improved technology based on the above-mentioned journal literature. Although the entire process cycle has been shortened to a large extent, it is still necessary to use methanol to remove TCA , and the amount of solvent used is large, and the purchase cost or preparation cost of pepsin used is much higher than that of neutral protease, resulting in higher cost of the whole process

Images