Preparation method of adenosylcobalamin

Abstract

The invention discloses a preparation method of adenosylcobalamin, which comprises the following steps: (1) filtering a fermentation liquid to obtain mycelia and a primary filtrate; (2) suspending the mycelia, breaking walls, and filtering to obtain a suspension filtrate; (3) exchanging the suspension filtrate with cation exchange resin, and replacing with weak alkali to obtain a cation column resolution liquid; (4) filtering to obtain a secondary filtrate; (5) adsorbing the secondary filtrate through macroporous resin columns, developing, and resolving to obtain a macroporous resolution liquid; (6) evaporating the macroporous resolution liquid to remove acetone, thereby obtaining a concentrated liquid; (7) exchanging the concentrated liquid with anion exchange resin to obtain a destaining solution; (8) adsorbing with macroporous resin, and resolving to obtain a crystalline stock solution; and (9) adding acetone to the crystalline stock solution, and carrying out dynamic crystallization and static crystallization to obtain the finished adenosylcobalamin product. Since the adenosylcobalamin is extracted and refined with full resin, the invention is simple to operate, has the advantages of high stability and environment friendliness, can greatly enhance the yield and quality of the adenosylcobalamin, and can lower the production cost.

Description

technical field [0001] The invention relates to the field of chemical industry and pharmacy, in particular to a method for preparing adenosylcobalamin. Background technique [0002] Adenosylcobalamin is a B vitamin 12 One of the series of products, chemical name: 5,6-dimethylbenzimidazole-5'-deoxyadenosyl cobalamin, is an octahedral cobalamin complex, molecular formula: C 72 h 100 CoN 18 o 17 P, molecular weight: 1579.60. Adenosylcobalamin is dark red crystal and non-crystalline powder with strong hygroscopicity and easy to decompose when exposed to light. Adenosylcobalamin is a B vitamin 12 An important variety of series products, which are raw materials and have important medical value. [0003] The preparation method of adenosylcobalamin mainly includes following several at present. [0004] One is a chemical method, that is, first convert the adenosylcobalamin produced by microbial fermentation into cyanocobalamin, and then add enzymes to convert it into adenosyl...

AI Technical Summary

Problems solved by technology

[0006] In addition, Hebei Huarong Pharmaceutical Co., Ltd. has applied for an invention patent with the patent number CN101948494 A, and the name is a method for extracting adenosylcobalamin. The method for extracting the aerobic fermentation broth is: hydrolyze the fermentation broth first, then extract adenosylcobalamin through macroporous resin and refine alumina. Glycobalamin enters the extracellular fluid with complex components, and then extracts the complex hydrolysis filtrate, which increases the difficulty of extraction, so the extraction yield is low, as follows: the yield of adenosylcobalamin is low, which is more than 50%; B 12 The total yield is low, only more than 80%

In addition, the alumina refining process is still used in the extraction process, which has low extraction capacity, large loss, low yield, high labor intensity, serious environmental pollution, and high production costs.