Real-time reverse transcription-polymerase chain reaction (RT-PCR) detection method and kit for Coxsackie virus

Abstract

The invention relates to a real-time reverse transcription-polymerase chain reaction (RT-PCR) detection method and a kit for a Coxsackie virus. Specifically, the invention discloses a method for detecting the Coxsackie virus, which is characterized in that a polymerase chain reaction is carried out in a polymerase reaction system. The polymerase reaction system comprises an amplification Coxsackie virus-contained specific primer pair and a Coxsackie virus specific probe. The invention also provides the corresponding kit. The invention is capable of sensitively, simply and conveniently detecting and verifying the Coxsackie virus.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and nucleic acid detection. Specifically, the present invention relates to a real-time fluorescent RT-PCR detection method and kit for Coxsackie virus detection. Background technique [0002] Coxsackievirus (Coxsackievirus) belongs to Picornaviridae (Picornaviridae) enterovirus genus, according to its biological characteristics can be divided into two categories, namely A group and B group. Coxsackievirus infection in humans causes herpetic angina (group A) and nonparalytic polio (group B), respectively. According to the survey, 79% of acute fevers accompanied by oropharyngeal herpes and rash are caused by Coxsackie virus A group, and the more common Coxsackie virus A16 type is one of the main pathogens causing hand, foot and mouth disease. Coxsackievirus group B is the main pathogenic microorganism causing viral myocarditis in humans [1] . [0003] In recent years, there have been ...

AI Technical Summary

Problems solved by technology

Previous studies were mostly based on Taqman-FAM-TAMRA probes, for example: Tana (2008) established a multiplex real-time fluorescent PCR method for Coxsackievirus A16 and Enterovirus 71 [5] However, the method used to detect A16 virus is a fluorescent hybridization probe, which has higher requirements for the real-time fluorescent PCR system, and not all real-time fluorescent PCR instruments can implement the reaction

Fluorescent hybridization probes require 2 adjacent probes, which increases cost, and one of the probes is labeled with the LC Red 750 fluorophore, which has limited resolution and poor sensitivity

Therefore, this method is not conducive to popularization and application

[0007] In this field, there is still a lack of highly sensitive, highly specific, flexible, simple, and highly applicable detection methods for a single serotype of Coxsackieviruses, and there is even a lack of detection techniques for rapid detection and identification of various serotypes of Coxsackieviruses

[0008] In summary, in view of the fact that there is no real-time fluorescent RT-PCR detection method with good specificity and high sensitivity for Coxsackie virus

Images