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Application of carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs

An anti-tumor drug, phycocyanin technology, applied in anti-tumor drugs, drug combinations, peptide/protein components, etc., can solve the problems of cytotoxicity, cell damage, death, etc., and achieve good growth inhibition and inhibition enhanced effect

Inactive Publication Date: 2011-12-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process is that first, the tumor tissue selectively absorbs the photosensitizer and stores it in it, and then the photosensitizer is activated after local irradiation with light of an appropriate wavelength, and the photosensitizer in the excited state transfers energy to the surrounding oxygen to generate active oxygen. Strong singlet oxygen, singlet oxygen and adjacent biomacromolecules undergo oxidation reactions, resulting in cytotoxicity, which in turn leads to cell damage and even death

Method used

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  • Application of carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs
  • Application of carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs
  • Application of carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of carbon nanotube-chitosan-phycocyanin composite (MWCNT-CS-PC)

[0032] (1) Weigh 40mg of carboxylated carbon nanotube powder with a diameter of 10nm (carboxylation rate 1wt%), add it to 150ml 5mM PBS solution, ultrasonically disperse for 60 minutes, then add 170mg water-soluble chitosan CS, and continue ultrasonic oscillation After 60 minutes, the mixed solution was magnetically stirred for 1 hour after sonication. After standing and stratifying for 6 hours, take the upper layer liquid and centrifuge at a high speed of 10000r / min. The upper layer liquid after centrifugation is the intermediate sample: chitosan-modified water-soluble multi-walled carbon nanotube homogeneous solution (MWCNT-CS) . Use a 30kD ultrafiltration tube to remove excess CS in MWCNT-CS, and freeze-dry the resulting filter cake as a sample for characterization analysis;

[0033] (2) Add 70 mg of carbodiimide hydrochloride (EDC-HCl) and 160 mg of N-hydroxysuccinimide (NHS) t...

Embodiment 2

[0038] (1) Tumor cell growth inhibition

[0039] The growth inhibitory effect of the complex on tumor cells was analyzed by MTT colorimetry.

[0040] The revived breast cancer MCF-7 cells and liver cancer HepG2 cell lines were placed in DMEM complete culture medium containing 10% fetal bovine serum, at 37°C, 5% CO 2 Subculture in an incubator under saturated humidity conditions. Cell concentration 3×10 3 / 100μL Inoculate two kinds of cancer cells in a 96-well culture plate, 200μl per well. At 37°C, 5% CO 2 Culture in the incubator for 24 hours to remove the original medium, then add 100 μL of fresh high-sugar DMEM complete medium without calf serum, and then add the MWCNT-CS-PC prepared in Example 1, the concentrations of which are respectively 30, 60, 90, 120, 150 (μg / mL), the medium wells without MWCNT-CS-PC were used as the negative control group, cultured for 24 hours, washed with phosphate buffer saline (PBS) for two time; then add 20μL 5mg.mL -1 MTT solution, cont...

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Abstract

The invention provides the application of carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs. The carbon nanotube-chitosan-phycocyanin complex is composed of multi-walled carbon nanotubes, Chitosan and spirulina phycocyanin are formed by non-covalent combination. The complex has good growth inhibitory effect on breast cancer MCF-7 cells and liver cancer HepG2 cells, especially under the irradiation of near-infrared light and visible light. The inhibitory effect was further strengthened, and all showed the characteristics of killing tumor cells. As an anti-tumor biomedical material, the carbon nanotube-chitosan-phycocyanin composite provided by the invention has broad prospects in photodynamic therapy and photothermal therapy of tumor cells.

Description

[0001] technical field [0002] The invention relates to the application of the phycocyanin complex, in particular to the application of the carbon nanotube-chitosan-phycocyanin complex in the preparation of antitumor drugs. Background technique [0003] In cyanobacteria cells, phycocyanin (Phycocyanin, PC) is a kind of ubiquitous photosynthetic auxiliary pigment, and it is a special pigment protein. Studies have shown that phycocyanin has significant anti-tumor activity, can improve the activity of lymphocytes, and enhance the body's ability to prevent and resist diseases. Phycocyanin has characteristic absorption peaks at wavelengths of 280nm, 360nm and 620nm. It can produce photodynamic in vitro killing effect on human cancer cells in vitro. [0004] Carbon nanotubes (carbon nanotubes, CNTs) have a unique nested cylindrical graphene structure, with diameters ranging from a few nanometers to hundreds of nanometers, and lengths ranging from a few nanometers to several mic...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K41/00A61K38/16A61P35/00
Inventor 张学武廖晓霞
Owner SOUTH CHINA UNIV OF TECH
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