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Method for preparing collagen peptides by using fresh yak bones

A collagen peptide and fresh bone technology, applied in the field of biopharmaceuticals, can solve problems such as insufficiently stable reaction speed, high requirements for equipment materials, and changes in enzyme activity, and achieve the effects of fast processing, low energy consumption by ultrafiltration, and easy absorption

Inactive Publication Date: 2011-11-02
苏州北商智业管理咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although both the acid method and the alkali method are rapid and thorough in hydrolysis, both methods have great limitations and have high requirements on the material of the equipment. The acid hydrolysis method will destroy all tryptophan, and the parts of serine and tyrosine It is destroyed, and the product yield is low, the equipment is severely corroded, and produces secondary pollution, while the alkaline hydrolysis method destroys all amino acids containing hydroxyl and sulfhydryl groups and produces racemization
The enzymatic method is not stable enough because of the change of pH in the reaction process, which leads to the change of enzyme activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Add 30kg of demyelinated yak fresh bone and add enzymatic hydrolysis irrigation, add 600L water, 50g trypsin, borate to adjust the pH to 7.5-8, stir the enzymatic hydrolysis at a constant temperature of 45°C for 5 hours, and filter the enzymatic hydrolysis solution once . Add 30g of flavor protease to the primary enzymolysis solution, stir the enzymolysis at a constant temperature of 50°C for 10 hours, and then warm to 75°C to inactivate the enzyme for 15 minutes to obtain the second enzymolysis solution. Add activated carbon and diatomaceous earth 1:1 mixed flocculant to precipitate impurities in the secondary enzymolysis liquid, filter through filter cloth, ultrafilter the filtrate through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 2000-3000D, concentrate the filtrate and spray dry it to obtain a powder Collagen peptide product with a purity of 98.7%.

Embodiment 2

[0031] Add 30kg of demyelinated yak fresh bones and add enzymatic hydrolysis irrigation, add 600L water, 40g trypsin, borate to adjust the pH to 7.5-8, stir the enzymatic hydrolysis at a constant temperature of 45°C for 6 hours, and filter the enzymatic hydrolysis solution once . Add 35g of flavor protease to the primary enzymolysis solution, stir the enzymolysis at a constant temperature of 50°C for 5 hours, and then warm to 75°C for 15 minutes to inactivate the enzyme to obtain the second enzymolysis solution. Add activated carbon and diatomaceous earth 1:2 mixed flocculant to precipitate impurities in the secondary enzymolysis solution, filter through filter cloth, ultrafilter the filtrate through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 2000-3000D, concentrate the filtrate and spray dry it to obtain a powder Collagen peptide product with a purity of 98.8%.

Embodiment 3

[0033] Add 30kg of demyelinated yak fresh bone and add enzymatic hydrolysis irrigation, add 600L water, 50g trypsin, borate to adjust the pH to 7.5-8, stir the enzymatic hydrolysis at a constant temperature of 45°C for 7 hours, and filter it with gauze to obtain an enzymatic hydrolysis solution . Add 30 g of flavor protease to the primary enzymolysis solution, stir the enzymolysis at a constant temperature of 50°C for 12 hours, and then warm to 75°C for 20 minutes to inactivate the enzyme to obtain the second enzymolysis solution. Add activated carbon and diatomaceous earth 1:1 mixed flocculant to precipitate impurities in the secondary enzymolysis solution, filter through filter cloth, ultrafilter the filtrate through a porous fiber ultrafiltration membrane with a molecular weight cut-off of 2000-3000D, concentrate the filtrate and spray dry it to obtain a powder Collagen peptide product with a purity of 99.3%.

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Abstract

The invention relates to a method for preparing collagen peptides using fresh yak bones. The final product of collagen peptides is obtained by hydrolyzing marrow-removed yak bones with enzymes twice, allowing precipitation, filtering, performing ultrafiltration and spray-drying. The preparation method adopts safe raw materials and has a simple and rapid process. The product has a low molecular weight and is easy to absorb. Besides, the cost is reduced and the low-carbon and environmentally-friendly effect is achieved.

Description

Technical field: [0001] The invention belongs to the field of biopharmaceuticals and relates to a method for preparing collagen peptides from fresh yak bones. Background technique: [0002] Collagen, also known as collagen, is a biological polymer with a triple helix structure synthesized by animal cells. It is the main protein that constitutes animal connective tissue and is widely found in bones, tendons, cartilage and skin. Its function is to maintain the shape and structure of skin and tissues and organs, and to repair various damaged tissues. In the composition of human skin, 70% is composed of collagen. The biggest difference between collagen and other proteins is the composition of special amino acids: glycine (20%), pro / hydroxyproline (25%) and glutamic acid (11%). Due to the special amino acid composition of collagen and the peculiarity of its sequence, it has a special effect on skin and bone and joint health. [0003] Collagen should not be supplemented directl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/12C07K1/34
Inventor 刘宝岩张翼李法庆
Owner 苏州北商智业管理咨询有限公司
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