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Method for simultaneously detecting thiourea and dulcin in flavor and fragrance by using high performance liquid chromatography

A high-performance liquid chromatography, flavor and fragrance technology, which is applied in the field of high-performance liquid chromatography for simultaneous determination of thiourea and glycosin in flavors and fragrances, can solve the problems of large analysis error, complicated operation steps, and high detection cost, and achieves easy operation. , good separation effect, good selectivity

Active Publication Date: 2011-10-26
CHINA TOBACCO ZHEJIANG IND
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Problems solved by technology

Liquid-mass spectrometry has the advantages of high sensitivity and qualitative accuracy, and mass spectrometry detectors are mostly used, so there is generally a problem of high detection costs
Spectrophotometry has simple equipment and low cost, but the operation steps are complicated and the precision is not high
The electrochemical analysis method is small in size, easy to operate, easy to carry, and can be used for on-site monitoring and low-cost test values, but it is greatly affected by temperature and humidity, and the accuracy is not high
Although capillary electrophoresis is efficient and fast, due to the small amount of sample in its analysis system, it may cause large analysis errors

Method used

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  • Method for simultaneously detecting thiourea and dulcin in flavor and fragrance by using high performance liquid chromatography
  • Method for simultaneously detecting thiourea and dulcin in flavor and fragrance by using high performance liquid chromatography
  • Method for simultaneously detecting thiourea and dulcin in flavor and fragrance by using high performance liquid chromatography

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Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Materials and reagents

[0025] Acetonitrile (chromatographically pure): Sinopharm Chemical Reagent Co., Ltd.; dipotassium hydrogen phosphate (A.R): Huzhou Chemical Reagent Co., Ltd.; flavor and fragrance: clove oil sample; thiourea standard: ACROS company (purity 99.0%); Product: APOLLO SCIENTIFIC LIMITED company.

[0026] 1.2 Instruments and equipment

[0027] Electronic analytical balance: Sartorius, Germany; ultrasonic cleaner: Kunshan Ultrasonic Instrument Co., Ltd.; Agilent1100 high performance liquid chromatography (equipped with diode array detector and Rev.A.10.02 chromatographic workstation, Agilent, USA).

[0028] 1.3 Method

[0029] 1.3.1 Configuration of standard solution

[0030] 1000μg / mL standard stock solution: Accurately weigh 0.1g of thiourea standard product in a 100mL volumetric flask, dissolve it with liquid chromatography mobile phase and adjust the volume to the mark to obtain 1000μg / mL thiourea standard s...

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Abstract

The invention discloses a method for simultaneously detecting thiourea and dulcin in flavor and fragrance by using high performance liquid chromatography, comprising the following steps: using high performance liquid chromatography diode array detection method to simultaneously detect thiourea and dulcin in flavor and fragrance, and calculating the contents of thiourea and dulcin in flavor and fragrance according to the standard curves of thiourea and dulcin. The conditions of the high performance liquid chromatography diode array detection method comprise: using a C18 chromatographic column, using acetonitrile and 0.02mol / L aqueous solution of dipotassium hydrogen phosphate as a mobile phase, and using a detection wavelength of 240-245 nm. The method can accurately carry out the qualitative and quantitative analysis of thiourea and dulcin in flavor and fragrance with good separating effect and wide linear range, with the advantages of simple, rapid, good selectivity, low cost and the like, and is suitable for a wide range of applications.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a method for simultaneously determining thiourea and glycin in flavors and fragrances by high performance liquid chromatography. Background technique [0002] Thiourea (Thiourea), namely thiourea, is an important chemical raw material, mainly used in organic synthesis, textile industry, metal processing and other industries. Because it has a certain bleaching function, it can also be used in the bleaching of textiles. However, in recent years, it has been reported that thiourea is added to foods such as wheat and noodles as a whitening agent and gluten-enhancing agent, endangering the dietary health of consumers. Thiourea can inhibit the thyroid gland and hematopoietic organs after being ingested into the human body, and has a certain stimulating effect on the upper respiratory tract and eyes. Therefore, it is necessary to strictly prohibit the addition of thiourea to food in ac...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36G01N30/60G01N30/06
Inventor 周国俊蒋健周示玉李祖光胡宝祥
Owner CHINA TOBACCO ZHEJIANG IND
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