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Foot and mouth disease virus antigen polypeptide, fusion antigen polypeptide and vaccine

A technology of foot-and-mouth disease virus and antigenic polypeptide, which is applied in the direction of viral peptides, anti-viral agents, peptides, etc., can solve the problems of difficulty in prevention and control of foot-and-mouth disease and inability to immunize each other.

Inactive Publication Date: 2011-09-14
SHANGHAI SHEN LIAN BIOMEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Because of the high variability of foot-and-mouth disease virus and the mutual immunity between each serotype, it brings great difficulties to the prevention and control of foot-and-mouth disease

Method used

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  • Foot and mouth disease virus antigen polypeptide, fusion antigen polypeptide and vaccine
  • Foot and mouth disease virus antigen polypeptide, fusion antigen polypeptide and vaccine
  • Foot and mouth disease virus antigen polypeptide, fusion antigen polypeptide and vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1. The solid-phase synthesis of polypeptide

[0064] Equipment: PSI 300B automatic peptide synthesizer, using Rink Amide MBHA resin as a solid phase carrier; Pall Centrasette tangential flow ultrafiltration system, using Omega series membrane cassettes.

[0065] Raw materials and reagents: 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid is used as the starting material, and other reagents used in the solid-phase synthesis are detailed in the following parts of the examples. All the raw materials and reagents used in the synthesis can be purchased in the market or obtained after simple preparation by those skilled in the art according to known techniques.

[0066] The antigenic polypeptide shown in SEQ ID NO.11 (referred to as antigenic polypeptide A) was synthesized by Merrifield solid-phase synthesis method.

[0067] 1. Polypeptide synthesis

[0068] The synthesis order of Merrifield solid-phase synthesis is from C-terminal to N-terminal. In the a...

Embodiment 2

[0087] Example 2. Preparation of Antigen Polypeptide Vaccine

[0088] 1. Water phase preparation

[0089] The antigen polypeptide A obtained in Example 1 was diluted to 50 μg / ml with sterilized water for injection, and filtered through a filter with a pore size of 0.2 μm.

[0090] 2. Preparation of oil phase

[0091] The oil phase adjuvant Montanide ISA 50 V2 was sterilized at 120°C for 30 minutes before use.

[0092] 3. Emulsification

[0093] First add the oil phase into the emulsification tank, then stir at 80-100r / min, and at the same time slowly add the water phase, the ratio of the oil phase / water phase is 1 / 1, stir for 2 minutes after adding, and then stir at 8500r / min After 6 minutes, it was emulsified to form a water-in-oil emulsion to obtain the foot-and-mouth disease vaccine of the present invention, which was designated as vaccine A.

Embodiment 3

[0094] Example 3. Efficacy Test of Polypeptide Vaccine

[0095] Test sample: vaccine A prepared in Example 2.

[0096] Experimental animals: healthy pigs with negative foot-and-mouth disease, weighing about 40kg / head, about 4 months old.

[0097] Test virus species: O / ZK / 93 foot-and-mouth disease virus strain (O type), which is provided by the Lanzhou Veterinary Research Institute of the Chinese Academy of Agricultural Sciences.

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Abstract

The invention provides a foot and mouth disease virus antigen polypeptide, a foot and mouth disease virus fusion antigen polypeptide and a foot and mouth disease virus vaccine containing the antigen polypeptide and / or the fusion antigen polypeptide. The invention further provides application of the antigen polypeptide, the fusion antigen polypeptide and the vaccine in prevention and control of foot and mouth disease virus infection. The foot and mouth disease virus antigen polypeptide, the fusion antigen polypeptide and the vaccine have broad-spectrum immunogenicity, and can generate good immunogenicity on different foot and mouth disease viruses and variant strains thereof.

Description

technical field [0001] The invention relates to a foot-and-mouth disease virus antigen polypeptide, a fusion antigen polypeptide, and a foot-and-mouth disease virus vaccine containing the above antigen polypeptide and / or fusion antigen polypeptide. The present invention also relates to the preparation and application of the above-mentioned antigen polypeptide, fusion antigen polypeptide and vaccine. Background technique [0002] Foot-and-mouth disease is an acute, febrile, highly contagious zoonotic disease caused by foot-and-mouth disease virus. Foot-and-mouth disease mainly affects cloven-hoofed animals, and its clinical diagnosis is characterized by blisters on the oral mucosa, hooves and breast skin. Adult animals infected with foot-and-mouth disease generally have a low mortality rate, but the morbidity rate can reach 100%. A large number of blisters will appear on the mouth and hooves of infected animals, and the spirit will be depressed, which will lead to a sharp dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/09C07K19/00C12N15/42C12N15/62A61K39/135A61P31/14G01N33/569
Inventor 陈智英聂东升
Owner SHANGHAI SHEN LIAN BIOMEDICAL CORP
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