Multifunctional microfluid control device for operating biochemical fluids in microfluid control chip automatically

Abstract

The invention provides a multifunctional microfluid control device for operating biochemical fluids in a microfluid control chip automatically. The device consists of a system control module, electric drivers of six paths of direct-current high voltages, hydraulic drivers of two paths of micro-injection pumps, dielectrophoretic devices of two paths of sine waves and program software. The multifunctional microfluid control device has the characteristics that: through arbitrary combination of six paths of the direct-current high voltages (disconnection, voltage / connection and grounding), two paths of the micro-injection pumps (front waiting, propelling / injection, back waiting and withdrawal) and two paths of the sine waves (front waiting, sine wave and back waiting), independent or / and synchronous programmable control, rapid switching and stable output, the automatic operation on different biochemical fluids in the microfluid control chips with different structures is facilitated; and biochemical analysis on the microfluid control chip with strong establishing adaptability and small error range is more flexible and more simple and convenient.

Description

Technical field: [0001] The invention belongs to the microfluidic instrument platform technology, and in particular relates to a multifunctional microfluidic device for automatically manipulating the biochemical fluid of a microfluidic chip, which integrates electric drive, hydraulic drive, dielectrophoresis and fully automatic control. Background technique: [0002] Microfluidic chip (microfluidic chip) has become an important platform for microanalysis research in the fields of biology and chemistry in recent years because of its advantages of low consumption, rapidity, and integration of various analysis functions. Since microfluidic chip analysis is carried out in a micron-scale separation and analysis channel with network characteristics on a chip of a few square centimeters, the sample preparation and injection involved in biochemical experiments can be realized through the rapid manipulation of nanoliter or even picoliter sample scale. , mixing, reaction, separation, ...

AI Technical Summary

Problems solved by technology

The disadvantages are: due to the inclusion of micro-movable parts, the difficulty of chip processing and high cost, the performance of the micro-valve is relatively poor, there are many problems such as low back pressure, leakage and dead volume, and the application effect is not satisfactory.

Its disadvantages are: the chip structure is complex, the driving force is small, and it is suitable for low conductivity (10 -12 -10 -6 s / m) fluid

The disadvantages are: due to the relatively difficult optical focusing and the "dispersion" of the magnetic field, the device preparation is complicated, and the driving mechanism remains to be further studied

The problem now is that due to the large differences in the flow characteristics of various biological fluids (such as cell fluids), chemical fluids, and between biological fluids and chemical fluids, the existing microfluidic manipulation technology is applied to biochemical analysis of microfluidic chips. Also there are following deficiencies: 1) the driving mode is single, and some of them (as optical drive, magnetic drive) just emphasize the special application of driving, and the versatility is not strong; 2) a small amount of combination drives also only have two kinds of driving modes, for example: (gravity ) Hydraulic drive-electric drive, and the equipment that constitutes the combined drive is mostly a simple combination of individual instruments, which has shortcomings such as inconsistent control response time and affecting the reproducibility of chip analysis

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