Flounders quintuplet inactivated vaccine and preparation method thereof

Abstract

The invention relates to a flounders quintuplet inactivated vaccine and a preparation method thereof. The main constituents of the vaccine are mixed by the inactivated bacteria of five bacteria such as the vibrio anguillarum, turbot vibrio, the vibro harveyi, the algae dissolving glue vibrio and the edwardsiella tarda. The preparation method comprises the steps of: respectively preparing the five bacteria into 109-1010cfu / mL of vibrio suspension liquid, inactivating the vibrio suspension liquid by formalin, performing the isovolumetric mixing according to the proportion of 1:1:1:1:1 to prepare the flounders quintuplet inactivated vaccine raw liquid, and evenly mixing the flounders quintuplet inactivated vaccine raw liquid with the 15-35mg / mL astragalus polysaccharides adjuvant raw liquid according to the proportion of 1:1. The flounders quintuplet inactivated vaccine is multiple in immunity effects, can be used for effectively preventing the infection of the five bacterial pathogenic bacteria after being vaccinated for once, and provides a basis for the development of the marine fish multi-germinal inactivated vaccine.

Description

Technical field: [0001] The invention belongs to the preparation technology of seawater fish vaccine, which is a technical method for enhancing the immune effect of the vaccine by increasing the species of inactivated fish pathogenic bacteria in the vaccine and adding astragalus polysaccharide. Background technique: [0002] Flounder and flounder are the main species of industrial mariculture in northern my country, and have made important contributions to the economic development of fishery in coastal areas of my country. In recent years, with the rapid expansion of the scale and output of the flounder aquaculture industry, diseases have become one of the main problems faced by aquaculture production. The frequent occurrence of diseases poses a direct threat to the survival rate of breeding, and the economic losses caused have restricted the healthy and stable development of the industry to a certain extent. [0003] The Yellow Sea Fisheries Research Institute of the Chine...

AI Technical Summary

Problems solved by technology

The long-term use of antibiotics will not only easily lead to drug resistance of pathogenic bacteria and increase the difficulty of subsequent disease prevention, but also the problem of drug residues is also related to the food quality and safety of commercial fish.

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