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Corayceps militaris polysaccharide two-stage fermentation synthesis process

A technology for the synthesis of Cordyceps militaris, which is applied in the field of synthesizing polysaccharides from Cordyceps militaris using a biological two-stage fermentation method, can solve the problems of low yield of biologically active substances, and achieve the effects of increased yield, significant benefits, and simple operation

Inactive Publication Date: 2011-08-17
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it mainly produces bacteria, and the production of biologically active substances (polysaccharides) is relatively low

Method used

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  • Corayceps militaris polysaccharide two-stage fermentation synthesis process
  • Corayceps militaris polysaccharide two-stage fermentation synthesis process
  • Corayceps militaris polysaccharide two-stage fermentation synthesis process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of two-stage fermentation synthesis technique of militaris sinensis polysaccharide of the present invention, it comprises the following steps:

[0039] a. Preserving the strains: the strains of Cordyceps militaris grown on the potato slant medium were stored in a refrigerator at 4°C for later use.

[0040] b. Activation of slant strains:

[0041] Strain activation: transfer the Cordyceps militaris strain preserved in the above step a to the potato slant medium with a sterilized inoculation shovel, cultivate in a 25°C incubator for 7 days to obtain the activated Cordyceps militaris strain, and set aside.

[0042] C. Seed culture: the Cordyceps militaris bacterial classification after the activation of the above step b is cut with a sterilized inoculation hook to cut about 1cm The slant hyphae of the band culture medium is transferred in the 250mL Erlenmeyer flask that 50mL seed culture medium is housed, in the shaker 25°C, 150r / min shaking culture for 4-5 days, t...

Embodiment 2

[0058] The two-stage fermentation culture process method of the present embodiment is different from Example 1 in that the nutritional components and the addition of the fermentation medium are as follows:

[0059]

Embodiment 3

[0061] The two-stage fermentation and cultivation process method of this embodiment is different from that of Example 1 in that during the fermentation and cultivation process, the seed liquid is inserted into the fermenter containing the fermentation medium with a 5% inoculation amount, and the fermentation and cultivation process includes two stages : The first-stage fermentation conditions include: fermenter temperature 25°C, ventilation rate 1vvm, stirring speed 100-150rpm, fermenter pressure 0.05MPa for 6-7 days; second-stage fermentation conditions: fermenter tank temperature 25°C , the ventilation rate is 0vvm (no ventilation), and the stirring speed is 20-30rpm, and the cultivation is carried out for 1-2 days.

[0062] Compared with the prior art, the present invention has the following advantages: according to the growth and metabolism characteristics of Cordyceps militaris, the present invention designs and establishes a two-stage fermentation culture process to synth...

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Abstract

The invention belongs to the microorganism field, particularly relates to a corayceps militaris polysaccharide two-stage fermentation synthesis process; the process comprises the following steps: corayceps militaris strains are vaccinated on a potato slant culture medium, are cultured at the constant temperature and then are put in a conical flash containing a seed culture medium for shaking culture; then the corayceps militaris strains are put in a fermentation tank containing a fermentation medium, the fermentation process is as follows: the corayceps militaris is cultured at constant temperature for four to five days in ventilating and stirring condition, which is mainly used for providing a culture condition suitable for growing of corayceps militaris thallus so that the corayceps militaris grows rapidly; then the corayceps militaris is cultured for two to three days at the constant temperature in the condition of ventilation and slightly stirring, a culture solution is centrifuged, and ethanol in two times of volume of is added into an obtained liquid supernatant which is mixed and put up for the night at the temperature of 4 DEG C; and precipitates obtained through secondary centrifugation is dried so as to obtain the corayceps militaris. The process is reasonable in design and simple in operation, is suitable for large-scale industrial production and can solve the situation of drug source shortage and has obvious benefits.

Description

technical field [0001] The invention relates to a process for artificially cultivating and synthesizing polysaccharides from Cordyceps militaris, in particular to a method for synthesizing polysaccharides from Cordyceps militaris by a biological two-stage fermentation method. Background technique [0002] Cordyceps militaris [Cordyceps militaris] (commonly known as Cordyceps militaris) is a complex of insects and fungi formed by the larvae of the Lepidoptera bat mothidae after being parasitized by Chinese hairy sporophytes. It is listed as one of the "Three Treasures of Traditional Chinese Medicine" in China. It contains a variety of active ingredients (such as Cordyceps mycelium, polysaccharides, cordycepin, etc.), which have the functions of enhancing immunity, anti-tumor, anti-oxidation and lowering blood sugar, and the market demand is huge. [0003] However, the growth cycle of wild Cordyceps sinensis is too long, and due to the indiscriminate digging and harvesting of...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/645
Inventor 崔建东张思孙立梅
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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