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Method for preparing astragalus polysaccharides

A technology of astragalus polysaccharides and astragalus, which is applied in the field of preparation of astragalus polysaccharides, can solve the problems of low yield and content, destruction of astragalus polysaccharides, and increased costs, and achieve the effects of increasing yield and content, wide molecular weight distribution, and reducing production costs

Active Publication Date: 2013-07-17
LUZHOU PINCHUANG TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After many operations, it was found that there are many problems in this extraction method: First, the long-time boiling and heating will inevitably destroy the astragalus polysaccharide by hydrolysis, resulting in the reduction of the pharmacological activity of the astragalus polysaccharide
In addition, the flavonoids and saponins in Astragalus will be extracted at the same time. The chemical formula of flavonoids and their analogs contains phenolic hydroxyl groups, which are easily oxidized and discolored in the air, which has a great impact on the purity and color of the product; secondly A total of 20-26 times the volume of water can be boiled for three times to extract the polysaccharide relatively cleanly. Such a large volume of water makes subsequent operations such as decompression and concentration a huge workload and low efficiency.
After a long time of boiling, the boiled liquid is flocculent, and it is difficult to achieve the separation effect by general filtration and ultrafiltration. Only centrifugation can be used to separate the clear liquid and residue, which will undoubtedly increase the workload and increase the cost.
In addition, the extraction method of traditional water boiling and alcohol precipitation has a yield of 4-7%, and the polysaccharide content is 30-40%, which is low in yield and content.

Method used

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  • Method for preparing astragalus polysaccharides
  • Method for preparing astragalus polysaccharides

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preparation example Construction

[0025] The preparation method of astragalus polysaccharide of the present invention comprises the following steps:

[0026] A, extract Astragalus with water to obtain water infusion;

[0027] B. Heating the water immersion liquid, discarding the bottom sediment after cooling to obtain the supernatant;

[0028] C. Concentrating the supernatant obtained in step B to a paste, adding ethanol for precipitation, and filtering to obtain the crude product of astragalus polysaccharide;

[0029] D. Refining the crude product of astragalus polysaccharide to obtain the refined product of astragalus polysaccharide.

[0030] The heating temperature in step B is 90-100° C., and the heating time is 15-20 minutes.

[0031] The extraction described in step A is diafiltration. Specifically, astragalus and water are added to the diafiltration column for circulatory diafiltration, the flow rate of the leachate is kept at 3-5ml / min, and the extraction time is 2-3 hours.

[0032] The dosage of As...

Embodiment 1

[0040] The preparation of embodiment 1 astragalus polysaccharide

[0041] The dried root of Astragalus membranaceus is washed, sliced, dried, pulverized by a pulverizer until passed through a 40-mesh sieve; weigh 100g of sieved dry powder of Astragalus membranaceus, add 250ml of water, stir and add it to the percolation column, adjust the pump speed to keep the leachate flow rate at 3ml / min, the extraction time was 3 hours, and the leachate was collected. Heat the obtained leachate in a water bath at 90°C for 15 minutes, let it stand to cool, and discard impurities such as denatured proteins in the bottom layer to precipitate. The supernatant was concentrated in a rotary evaporating dish under reduced pressure until it became a gelatinous paste, and then 3 times of 95% ethanol was added for precipitation to obtain 18.09 g of crude astragalus polysaccharide. The crude polysaccharide yield was 18.09%, and the polysaccharide content was 65.32%.

[0042] The crude astragalus po...

Embodiment 2

[0044] The preparation of embodiment 2 astragalus polysaccharides

[0045] Wash, slice and dry the dried roots of Astragalus membranaceus, crush them through a 60-mesh sieve; weigh 100g of sieved Astragalus membranaceus dry powder, add 250ml of water, stir and add to the percolation column, adjust the pump speed to keep the leachate flow rate at 4ml / min , the extraction time was 2.5 hours, and the leachate was collected. Heat the obtained leachate in a boiling water bath for 20 minutes, let it stand for cooling, then discard the denatured protein and other impurities in the bottom layer to precipitate. The supernatant was concentrated in a rotary evaporator under reduced pressure until it became a gelatinous paste, and then 3 times of 95% ethanol was added for precipitation to obtain 21.79 g of crude astragalus polysaccharide, with a crude polysaccharide yield of 21.79% and a polysaccharide content of 68.10%.

[0046] The crude astragalus polysaccharide is purified by ion excha...

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Abstract

The invention belongs to the technical field of natural medicines, and in particular relates to a method for preparing astragalus polysaccharides. The astragalus polysaccharides prepared by the method are high in yield and purity. The method comprises the following steps of: A, extracting astragalus with water to obtain water extract; B, heating the water extract, cooling, and discarding sediments on the bottom layer to obtain supernate; C, concentrating the supernate obtained in the step B into paste, adding ethanol for precipitation, and filtering to obtain crude astragalus polysaccharides;and D, refining the crude astragalus polysaccharides to obtain refined astragalus polysaccharides. The yield of the refined astragalus polysaccharides extracted by the method is 12 to 15 percent, thepolysaccharide content is over 94 percent, and the astragalus polysaccharides can be used for preparing immune medicines and health products. The yield of the polysaccharides in the method is improved by 8 to 11 percent compared with that of the traditional water extraction and ethanol precipitation method, the utilization rate of dry astragalus roots is improved and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, in particular to a preparation method of astragalus polysaccharide. Background technique [0002] Astragalus polysaccharide is an active polysaccharide in the dried root of Astragalus mongolica or Astragalus membranaceus. Its main biological activities are as enhancing immune function, anti-influenza, anti-tumor, anti-aging and anti-radiation, improving the myocardial contractility of dogs with acute infarction, reducing the infarct size, and reducing myocardial damage. [0003] With the in-depth research on the physiological activity of astragalus polysaccharide, many of its pharmacological effects have been gradually discovered and confirmed. In recent years, the application of APS has become more and more extensive. The more common products include APS health products, APS feed additives, and oral and injection preparations of APS. Because of its excellent performance in enhancing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P9/10A61P17/16A61P31/16A61P35/00A61P37/04A61P39/00A61P39/06A23K1/16A23L1/09A23K20/163A23L33/125
Inventor 丁海龙易彬卢中明敖灵
Owner LUZHOU PINCHUANG TECH CO LTD
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