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Pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and method thereof

A solid fermentation device and solid fermentation technology, which are applied in sterilization methods, solid-phase fermentation bioreactors, and methods of supporting/fixing microorganisms, etc. and other problems, to achieve the effect of shortening fermentation time, small investment and good effect

Inactive Publication Date: 2011-06-22
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is, in order to overcome the problems such as the bacterial contamination of solid fermentation in the prior art, and difficult to realize mass transfer and heat transfer between thalline and culture medium, thus propose a kind of gas-phase inoculation and supplementary purebred culture method Solid fermentation device and method thereof
[0009] The present invention will start from the various links of the fermentation process such as medium sterilization, aseptic inoculation, cultivation and feeding, and design a complete solid-state fermentation aseptic culture device and method to overcome the problem of bacterial contamination in the solid-state fermentation process

Method used

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  • Pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and method thereof
  • Pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and method thereof

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Experimental program
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Effect test

Embodiment 1

[0045] The strain of solid fermentation is A. vinelandii. First configure the liquid culture medium of nitrogen-fixing bacteria, add it into the seed tank 3, open the disinfection valve 16 and feed steam to eliminate the culture medium. After cooling, insert the bacterial suspension obtained from the inclined plane and cultivate at 30°C with a stirring speed of 130rpm. After culturing for 10 hours, a liquid strain with good fluidity was obtained. Prepare solid culture medium during this period (preparate liquid culture medium first, then add peat moss according to solid-liquid ratio 1: 3), be placed on the shallow plate of solid fermenter 1, material layer height is 2cm. Open the gas path end valve 15 and use steam to sterilize the solid medium at 120°C for 30min, and then cool it down to 30°C.

[0046] After the cultivation of the liquid bacteria is completed, turn on the air compressor, valves 15, 12 at the gas path and valve 11 at the discharge end, and adjust the air spe...

Embodiment 2

[0048] The strain of solid fermentation is A. vinelandii. First configure the liquid culture medium of nitrogen-fixing bacteria, add it into the seed tank 3, open the disinfection valve 16 and feed steam to eliminate the culture medium. After cooling, insert the bacterial suspension obtained from the inclined plane and cultivate at 30°C with a stirring speed of 130rpm. After culturing for 10 hours, a liquid strain with good fluidity was obtained. Prepare solid culture medium during this period (preparate liquid culture medium first, then add peat moss according to solid-liquid ratio 1: 3), be placed on the shallow plate of solid fermenter 1, material layer height is 2cm. Open the gas path end valve 15 and use steam to sterilize the solid medium at 120°C for 30min, and then cool it down to 30°C.

[0049] After the cultivation of the liquid bacteria is completed, turn on the air compressor, and the valve 15 at the gas path end, the valve 12 at the gas path end, and the valve 1...

Embodiment 3

[0053] The fermentation strain is Penicillium decumbens.

[0054] Cultivate the seed solution first, and after cultivating at 30°C for 20 hours, adjust the air flow rate to 2.0m 3 / hr, inoculated onto the solid medium (with the wheat straw with a particle diameter of about 2mm as the solid matrix), the material layer thickness of the solid medium is 3cm, and the inoculum size is 10%. The culture is carried out by the method of periodic variable pressure, the pressure is periodically varied between 0.5MPa and 1.8MPa, and the high and low pressure are maintained for 3 minutes and 3 minutes respectively. Keep the humidity of the reactor at 50-80% during the continuous fermentation. If the humidity is lower than the set value, the sterilized sterile water will be sent into the reactor in the form of mist through the airflow. The whole fermentation cycle is 114hr, β-Gase reaches 38.85IU g -1 .

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Abstract

The invention relates to a pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and a method thereof, and the apparatus is characterized in that the apparatus comprises a venturi tube, wherein the inlet end of the venturi tube is connected with an air filter through an air circuit valve; the outlet end of the venturi tube is connected with a solid-state fermentation cylinder which is provided with a pressure release valve; the throat segment of the venturi tube is connected with the outlet ends of a seed pot and a feeding pot through the valves of outlet ends of the seed pot and the feeding pot, respectively; the air circuit between the air circuit valve and the venturi tube is connected with a steam generator, the inlet end of the feeding pot, and the inlet end of the liquid seed pot through valves of the air circuit ends of the stream generator, the feeding pot, and the liquid seed pot, respectively; when high speed air flows through the venturi tube, the air-flow speed at the throat segment of the venturi tube increases to form a negative pressure to absorb the liquid seed or material supplement; and the liquid seed or feed liquid is mixed with the gas to form aerosol, which is injected into the solid substrate in the solid fermentation cylinder, and aseptic inoculation and aseptic feeding are finished.

Description

technical field [0001] The invention belongs to the technical field of solid-state fermentation, and in particular relates to a solid-state fermentation device and a method for gas-phase inoculation and feeding of pure breed culture. Background technique [0002] Solid-state fermentation refers to the process in which microorganisms grow, reproduce and metabolize on a solid medium with no or almost no free water. Compared with liquid fermentation, solid-state fermentation has many advantages, such as: simple medium and wide sources; low investment, low energy consumption; high product yield, simple post-extraction process, etc. [0003] The solid-state fermentation technology in the modern sense is mostly a purebred cultivation process, which needs to be produced under the condition of no bacterial contamination. The so-called fermentation contamination refers to that during the fermentation process, other microorganisms other than the production strains invade the fermenta...

Claims

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Application Information

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IPC IPC(8): C12M1/16C12M1/12C12P1/00A61L2/07
Inventor 莫海涛张小勇肖传绪李佐虎
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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