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Chemiluminescence quantitative detection kit for estradiol

A chemical and quantitative detection technology for estradiol, which is applied in the field of estradiol quantitative detection kits, can solve problems such as difficult promotion, high price, and complicated operation, and achieve the effect of easy promotion and stable detection ability

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign automatic chemiluminescent enzyme immunoassay analyzers and matching kits are mostly used in closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Preparation of E2 quantitative detection kit

[0018] (1) Preparation of anti-E2 antibody-coated plates

[0019] a. Coating: Take 1mol / L Na 2 HPO 4 77.4ml and 1mol / L NaH 2 PO 4 Mix 22.6ml, add deionized water to 1000ml to form a 10-fold coating solution, dilute ten times before use, add an appropriate amount of anti-E2 monoclonal antibody (purchased from Meridian lifescience) and mix well, then add to the microplate Well, 100μl / well, 4°C for 16 hours;

[0020] b. Sealing: Discard the coating solution, pat dry on absorbent paper, add 3% BSA and 0.05% preservative (Proclin TM 300) of phosphate buffer (pH 7.4), 200 μl / well, 37 ° C for 2 hours;

[0021] c. Seal the bag: Discard the sealing solution, pat it dry on absorbent paper, pump it in a vacuum oven for 5 hours at room temperature, immediately vacuum seal the bag, check for air leakage, and re-seal the bag if there is a label. Store at 2-8°C.

[0022] (2) Preparation of enzyme markers

[0023] ...

Embodiment 2

[0036] Embodiment 2: the usage method of E2 quantitative detection kit

[0037] 1. Reagent and Sample Preparation

[0038] (1) Reagent preparation

[0039] a. Equilibrate the kit at room temperature (18-26°C) for 20 minutes.

[0040] b. Take out the concentrated washing solution from the kit, dilute it 1:20 with fresh purified water and add it to the washing solution bottle of the plate washer.

[0041] (2) Sample preparation

[0042] The qualified serum or plasma to be tested should be equilibrated at room temperature (18-26° C.) for 20 minutes before use.

[0043] 2. Operation steps

[0044] a. Take out the required E2 antibody-coated microwell plate and place it on the microwell rack

[0045] b. Add calibrators 1-6, quality control substances 1-2 and samples to be tested, 20 μl per well.

[0046] c. Add enzyme marker 100 μl / well.

[0047] d. Gently oscillate to mix.

[0048] e. Incubate at room temperature for 35 minutes.

[0049] f. Discard the waste liquid in the w...

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Abstract

The invention discloses a chemiluminescence quantitative detection kit for estradiol. The kit comprises an estradiol detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with an estradiol antibody. The kit can specifically and quantitatively detect the content of the estradiol in patient serum, and is used for the auxiliary diagnosis of diseases such as ovarian hypoplasia, primary ovarian failure, pituitary amenorrhea or infertility, Cushing's syndrome, Addison's disease, malignant tumors, focal lesion of brain and hypophysis, and the like. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and specifically relates to a kit for quantitative detection of estradiol (E2) and related preparation methods and usage methods. Background technique [0002] Estradiol (E2) is an 18-carbon steroid hormone with a benzene ring in the A ring, with a molecular weight of 272.4 Da, and is the main component of human estrogen. 70% of estradiol in the blood is combined with sex hormone-binding globulin (SHBG), 25% is combined with plasma albumin, and the rest is free. E2 is mainly produced by ovarian follicles, corpus luteum and placenta during pregnancy, and a very small amount is synthesized by testis or is a metabolite of testosterone, and becomes the main source of male estrogen. Another component of estrogen, estriol (E3), is a metabolite of E2 degraded in the liver except that it can be directly secreted by the placenta during pregnancy. In ad...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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