Culture method for efficiently inducing accumulation of nannochloropsis oculata fat

A technique of Nannochloropsis and fat is applied in the culture field of efficiently inducing fat accumulation in Nannochloropsis, which can solve the problems of less research on Nannochloropsis, and achieve the effect of accelerating division and improving fat accumulation.

Inactive Publication Date: 2011-05-25
北京芳能科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nannochloropsis belongs to Macrophthalmia Chlorophyta, is a seawater eukaryote with spherical cells, cell size 2-5μm, one chromophore, light green, lateral growth, light orange-red eyespots, when growing well , very deep chromatophores, no protein nucleus, 1-3 starch granules, extremely thin cell walls, small cell granules, fast growth and reproduction of Nannochloropsis, simple culture conditions, large-scale cultivation, and great commercial development potential. However, there are few studies on Nannochloropsis in related fields at present, and the application is mostly limited to the bait of marine organisms

Method used

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  • Culture method for efficiently inducing accumulation of nannochloropsis oculata fat
  • Culture method for efficiently inducing accumulation of nannochloropsis oculata fat
  • Culture method for efficiently inducing accumulation of nannochloropsis oculata fat

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Experimental program
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Effect test

Embodiment 1

[0049] 1. The formula of FN17 medium is as follows:

[0050] Sodium chloride 35g / L Sodium nitrate 160mg / L

[0051] Ferric chloride 4.5mg / L Disodium edetate 2.5mg / L

[0052] Sodium dihydrogen phosphate 12.0mg / L Trace elements 1ml / L

[0053] The preparation method of trace elements is as follows: take 11 mg of copper sulfate, 25 mg of zinc sulfate, 22.5 mg of cobalt chloride, and 220 mg of manganese chloride and add distilled water to 1 L.

[0054] Solution A:

[0055] Sodium Nitrate 290g / L Trace Elements 1ml / L

[0056] Wherein the trace element formula is:

[0057] Copper sulfate 1.5g / L Zinc sulfate 4.5g / L

[0058] Cobalt chloride 3g / L Manganese chloride 35g / L

[0059] Add water to make up to 1L

[0060] Solution B:

[0061] Sodium dihydrogen phosphate 190g / L

[0062] Solution C:

[0063] Ferric chloride 7g / L

[0064] Solutions A:B:C were mixed according to the ratio of 18:1:18, and the daily addition amounts were 6ml / L, 8ml / L and 10ml / L respectively. Dissolve the a...

Embodiment 2

[0093] 1. Multi-conditional induction of ultrasound and culture conditions

[0094] In a 300L small-scale photoreactor system, add FN17 medium, inoculate Nannochloropsis-X57 with a medium volume ratio of 1:8, and the inoculation concentration is 2.1×10 5 cells / ml, the culture temperature is 26-28°C, the light is 15000-18000 lux, the light time is 14 hours / day, and 0.8ml / L of the mixed solution of solution A, B and C is added every day (solution A: B: C according to 18:1:18 ratio), cultured for 8 days, with a frequency of 18kHz, a power of 4w, a treatment time of 15s, ultrasonic treatment twice, each time interval of 10s, and then flow rate of 1.0-1.5L / min into the concentration of 5% CO 2 Add the mixed solution of solution A and B every day (solution A: B is mixed according to the ratio of 15: 1), the addition amount is 30 μl / L, the amount of solution C added at one time is 1.2ml / L, after induction culture for 2 days, then With a frequency of 10kHz, a power of 3w, a treatmen...

Embodiment 3

[0101] In a 300L small photoreactor system, add FN17 medium, inoculate Nannochloropsis-X57 with medium volume ratio of 1:8, and the inoculation concentration is 2.21×10 5 cells / ml, the culture temperature is 26-28°C, the light is 15000-18000 lux, the light time is 14 hours / day, and 0.8ml / L of the mixed solution of solution A, B and C is added every day (solution A: B: C according to 18:1:18 ratio), cultured for 7 days, with 35kHz frequency, 6w power, treatment time 60s, sonication once, and then 2% CO at a flow rate of 0.5-1.0L / min 2 , add the mixed solution of solution A and B every day (solution A: B is mixed according to the ratio of 10:1), the amount added is 100 μl / L, the amount of solution C added at one time is 0.5ml / L, after 2 days of induction culture, 20kHz The frequency, the power of 5w, the treatment time of 15s, ultrasonic treatment once, induced fat accumulation for 4 days.

[0102] The results show that Nannochloropsis-X57 grows rapidly with this method, and en...

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Abstract

The invention provides a culture method for efficiently inducing accumulation of nannochloropsis oculata fat. The culture method is as follows: preparing a culture medium and daily-added nutrient liquid; carrying out biomass accumulation culture on annochloropsis oculata for 5-8 days; introducing CO2 in annochloropsis oculata culture liquid, and regulating the concentration of the daily added nutrient liquid; and carrying out ultrasonic processing on the annochloropsis oculata culture liquid one to three times at each interval of 10 seconds and for 5-60 seconds per time by using an ultrasonic instrument under a certain frequency, so that the accumulation of the nannochloropsis oculata fat is induced. The biomass of the nannochloropsis oculata cultured by the method is 1.5-2 times as much as that of the prior art, the content of oil in the nannochloropsis oculata cultured by the method is improved by 40%-65% compared with the prior art, and the growing period of the nannochloropsis oculata cultured by the method is shortened by 2-3 days; and in the invention, carbon source is supplemented in a form of introducing CO2, and a large amount of CO2 waste gases generated in the industrial production process are absorbed in the process of the large-scale production of the nannochloropsis oculata for inducing the accumulation of fat, thus the culture method has an important significance for energy conservation and emission reduction as well as environment protection.

Description

Technical field: [0001] The invention relates to a method for efficiently increasing the fat content of Nannochloropsis-X57 and cultivating Nannochloropsis-X57 in high density. Background technique [0002] With the continuous expansion of global energy demand, the search for clean energy that can replace petroleum is currently a hot spot of general concern. Biodiesel (fatty acid methyl ester) is a proven fuel, which has attracted worldwide attention as a renewable and environmentally friendly fuel energy. Biodiesel has excellent environmental protection characteristics. Emissions can be reduced by 90%, and carbon monoxide emissions can be reduced by about 10%. In addition, biodiesel raw materials come from photosynthesis, CO 2 The net emissions of biodiesel are zero, so the production and use of biodiesel will not cause greenhouse effects. In the United States, biodiesel is mainly based on soybeans. Crazy tree oil, etc., but can only meet a very small part of the current ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N13/00C12R1/89
Inventor 单东杰苏娇娇
Owner 北京芳能科技有限公司
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