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Method for preparing tissue engineering frame

A technology for tissue engineering scaffolds and degradable polymers, applied in medical science, prostheses, etc., can solve problems affecting the formation and repair of new tissues, destroying the biological activity of cells and tissues, and the inflammatory response of surrounding tissues

Active Publication Date: 2011-05-25
CHANGCHUN SINOBIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvents and cross-linking agents used in the prior art are difficult to remove, resulting in cytotoxicity when tissue engineering scaffold materials are used, causing inflammatory reactions in surrounding tissues, destroying the biological activity of cells and tissues, and affecting the formation of new tissues and repair effect

Method used

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  • Method for preparing tissue engineering frame
  • Method for preparing tissue engineering frame
  • Method for preparing tissue engineering frame

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Experimental program
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preparation example Construction

[0034] The embodiment of the present invention discloses a preparation method of a tissue engineering scaffold, comprising:

[0035] Step a) spinning the degradable polymer to obtain degradable polymer fibers;

[0036] Step b) putting the degradable polymer fiber into the tissue engineering scaffold mold, and then putting the tissue engineering scaffold mold equipped with the degradable polymer fiber into supercritical CO with a pressure of 7.38MPa-50MPa 2 Medium treatment, control the treatment temperature at 30-350°C, and then depressurize and discharge CO 2 , removing the tissue engineering scaffold mold to obtain the tissue engineering scaffold.

[0037] The degradable polymers include polylactic acid, polylactic acid derivatives, polylactide glycolide, polylactide glycolide derivatives, polylactic acid and inorganic particle composites, polycaprolactone , polycaprolactone derivatives, polycarbonate and polycarbonate derivatives, when the degradable polymer is several of...

Embodiment 1

[0049] Polylactic acid (PLA) is spun by a melt spinning method to obtain polylactic acid fibers;

[0050] Put the polylactic acid fiber into the hollow tubular mold of the tissue engineering support, then put the hollow tubular mold of the tissue engineering support into the high-pressure reaction kettle 103, put the reaction kettle 103 into the oil bath, and adjust the temperature to a constant temperature of 55 ℃;

[0051] Open the gas valve of the carbon dioxide steel cylinder 101, open the inlet valve of the critical fluid pump 102 and the reactor 103, close the vent valve of the reactor 103, and pass into the supercritical CO in the reactor. 2 , and repeated ventilation 3 times, adjust the supercritical fluid pump output CO 2 Keep the pressure constant to 20MPa, keep the reactor at a constant temperature of 55°C, and keep the constant pressure at 20MPa for 1 hour;

[0052] After the treatment is completed, the CO is decompressed and discharged 2 , the control time is 1...

Embodiment 2

[0055] Spinning polylactide glycolide (10% HA / PGLA) grafted with 10% hydroxyapatite by melt spinning;

[0056] Put the product obtained by spinning into the hollow tubular mold of the tissue engineering support, then put the hollow tubular mold of the tissue engineering support into the high-pressure reaction kettle 103, put the reaction kettle 103 into the oil bath, adjust the temperature and constant temperature to 50°C;

[0057] Open the gas valve of the carbon dioxide steel cylinder 101, open the inlet valve of the critical fluid pump 102 and the reactor 103, close the vent valve of the reactor 103, and pass into the supercritical CO in the reactor. 2 , and repeated ventilation 4 times, adjust the supercritical fluid pump output CO 2 Keep the pressure constant to 20MPa, keep the reactor at a constant temperature of 50°C, and keep the constant pressure at 20MPa for 1 hour;

[0058] After the treatment is completed, the CO is decompressed and discharged 2 , the control ti...

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Abstract

The embodiment of the invention discloses a method for preparing a tissue engineering frame. The tissue engineering frame is obtained by processing biodegradable polymer fibers in supercritical CO2 and by controlling processing temperature and processing temperature. The supercritical CO2 has certain dissolvent properties and can generate a micro-melting effect on the surfaces of the biodegradable polymer fibers, so when CO2 is discharged by decompression, the molten parts of contacted surfaces of biodegradable polymer fibers are solidified and crosslinked together to form the tissue engineering frame with high mechanical performance. Thus, the preparation method provided by the invention can complete the crosslinking and melting of fibers at a relatively lower temperature without dissolvent, crosslinker and the like, and generates no cytotoxicity.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing a tissue engineering scaffold. Background technique [0002] Tissue engineering is a science that applies the principles of cell biology, biomaterials and engineering to study bioactive substitutes for repairing or improving the structure of human diseased tissues or organs, with the purpose of repairing and regenerating damaged tissues or organs , to help patients restore the function of damaged tissues. The basic principle and method of tissue engineering is to put the osteoblasts obtained from the soft and hard tissues of the patient into the culture medium, and then expand the high-concentration tissue cells cultured in vitro and adsorb them on a biocompatible and On extracellular matrix materials that can be gradually degraded and absorbed by the human body. The matrix material is formed into a prefabricated tissue engineering scaffold to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18
Inventor 章培标崔立国陈学思王宗良王宇
Owner CHANGCHUN SINOBIOMATERIALS
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