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Organic-inorganic complex dispersion, cell culture substratum manufactured by using the dispersion, and manufacturing methods for same

一种无机复合物、制造方法的技术，应用在一般培养方法、细胞培养支持物/涂层、生物化学设备和方法等方向，能够解决无法细胞回收等问题

Active Publication Date: 2013-07-03
DIC CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] However, the polymer hydrogel described in this prior art is a bulk, and does not relate to an organic-inorganic composite dispersion liquid in which particles of an organic-inorganic composite are dispersed in an aqueous medium, which is excellent in film-forming ability.
In addition, when used as a cell culture substrate, although the cultured cells can be peeled off with tweezers, all the cultured cells cannot be recovered by naturally peeling off by temperature changes.

Method used

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  • Organic-inorganic complex dispersion, cell culture substratum manufactured by using the dispersion, and manufacturing methods for same
  • Organic-inorganic complex dispersion, cell culture substratum manufactured by using the dispersion, and manufacturing methods for same
  • Organic-inorganic complex dispersion, cell culture substratum manufactured by using the dispersion, and manufacturing methods for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0195] [Preparation of Solution (G) Dissolving Photopolymerization Initiator (D) in Solvent (E)]

[0196] 9.8 g of ethanol as a solvent (E) and 0.2 g of 1-hydroxycyclohexyl phenyl ketone "IRGACURE 184" (manufactured by Ciba-Geigy Corporation) as a water-insoluble photopolymerization initiator (D) were uniformly mixed to prepare a solution (G1).

[0197] [Preparation of reaction solution (F) containing monomer (a), water-swellable clay mineral (B), water-insoluble photopolymerization initiator (D), and aqueous medium (W)]

[0198] 1.3 g of 2-methoxyethyl acrylate (manufactured by Toagosei Co., Ltd.) as the monomer (a), and Laponite XLG (manufactured by Rockwood Addtitives Ltd.) as the clay mineral (B), a water-swellable synthetic hector Stone) 0.04 g, 25 μl of a solution (G1) as a water-insoluble photopolymerization initiator (D), and 10 g of water as an aqueous medium (W) were uniformly mixed to prepare a reaction liquid (F1).

[0199] [Preparation of organic-inorganic compo...

Embodiment 2

[0206] [Preparation of reaction solution (F) containing monomer (a), water-swellable clay mineral (B), water-insoluble photopolymerization initiator (D), and aqueous medium (W)]

[0207] 0.64 g of 2-methoxyethyl acrylate (manufactured by Toagosei Co., Ltd.) as the monomer (a), 0.12 g of Laponite XLG (manufactured by Rockwood Addtitives Ltd.) as the clay mineral (B), and 0.12 g as the water-insoluble 25 μl of a solution (G1) of a photopolymerization initiator (D) and 10 g of water as an aqueous medium (W) were uniformly mixed to prepare a reaction solution (F2).

[0208] [Preparation of organic-inorganic composite dispersion]

[0209] While stirring the above reaction solution (F2) with a magnetic stirrer, the intensity of ultraviolet light at 365nm for 180 seconds was 40mW / cm 2 Ultraviolet rays are used to prepare a slightly opalescent organic-inorganic composite dispersion (MNC1.5M0.5).

[0210] The particle size distribution of the organic-inorganic composite dispersion (M...

Embodiment 3

[0214] [Preparation of reaction solution (F) containing monomer (a), water-swellable clay mineral (B), water-insoluble photopolymerization initiator (D), and aqueous medium (W)]

[0215] 1.0 g of 2-methoxyethyl acrylate (manufactured by Toagosei Co., Ltd.) as the monomer (a), 0.2 g of Laponite XL G (manufactured by Rockwood Addtitives Ltd.) as the clay mineral (B), and 0.2 g as the non-water-soluble 25 μl of the solution (G1) of the photopolymerization initiator (D) and 10 g of water as the aqueous medium (W) were uniformly mixed to prepare a reaction solution (F3).

[0216] [Preparation of organic-inorganic composite dispersion]

[0217] While stirring the above reaction solution (F3) with a magnetic stirrer, the intensity of ultraviolet light at 365nm for 180 seconds was 40mW / cm 2 Ultraviolet rays are used to prepare a slightly opalescent organic-inorganic composite dispersion (MNC2.5M0.8).

[0218] The particle size distribution of the organic-inorganic composite dispersi...

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Abstract

Disclosed are: an organic-inorganic complex dispersion improved in film formability and adhesion to a base material. The organic-inorganic complex dispersion comprises an aqueous medium and particles of a complex dispersed in the aqueous medium, wherein the complex has a three-dimensional network structure formed by a polymer of a monomer comprising a monomer represented by general formula (1) and at least one inorganic material selected from a water-swellable clay mineral and silica. Also disclosed is an antifogging material manufactured by using the organic-inorganic complex dispersion. Further disclosed is a cell culture substratum improved in the detachability of cells cultured on the substratum, which is manufactured by using the organic-inorganic complex dispersion. Still further disclosed are manufacturing methods for same. [In the formula, R1 represents a hydrogen atom or a methyl group; R2 represents an alkylene group having 2 to 3 carbon atoms; R3 represents a hydrogen atom or an alkyl group having 1 to 2 carbon atoms; and n represents a number of 1 to 9.]

Description

technical field [0001] The present invention relates to an organic-inorganic composite dispersion in which particles of a composite of a polymer containing a (meth)acrylate monomer and a water-swellable clay mineral are dispersed in an aqueous medium, and a cell culture substrate manufactured using the same, and their method of manufacture. Background technique [0002] By combining organic polymers such as polyamide, polystyrene, polypropylene, polyimide, and polyurethane with clay, polymer composites called nanocomposites can be produced. It has been reported that, for the obtained polymer composite, by finely dispersing a clay layer having a large aspect ratio (aspect ratio), the modulus of elasticity, heat distortion temperature, gas permeability, and burning speed, etc. can be effectively improved (see, for example, Patent Document 1). [0003] As the amount of clay mineral contained in the polymer composite, it is desirable to have a high clay mineral content from th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L33/14C08F2/44C08K3/34C12M1/00
CPCC12N5/0068C12N2533/30C08F2/44C08F220/56C12N2533/10
Inventor 高田哲生原口和敏
Owner DIC CORP
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