Electrophoretic chip for optical detection and electrophoretic device thereof

Abstract

The invention relates to a capillary electrophoretic chip for optical detection and an electrophoretic device having the same. The chip is combined by two substrates, and the material of the substrate is an optically transparent polymer. An electrophoretic separation microstructure and an optical microstructure are created on the substrate A. The optical microstructure is created on the reflecting surface of the side of the electrophoretic separation channel to reflect the detection beam into the separation channel, and the detection beam enters from the side of the separation channel so as to excite the fluorescence of a sample. The fluorescence is detected in a direction perpendicular to the substrate surface, which is also perpendicular to the detection beam, so the interference from the excited light is reduced. The other substrate B as a cover plate is covered on the substrate A. The electrophoretic device comprises an electrophoretic chip, a high pressure module, a light source module, an exciting optical module, a collecting optical module, an optical filter, a photoelectric detector, a data reception module, and a processing and control module. The invention is applicable to high sensitive optical detection by creating an optical microstructure on the chip and combining with the electrophoretic device. The optical microstructure and the separation microstructure on the chip can be completed in one creating step, which facilitates process simplification, cost reduction, and batch production.

Description

technical field [0001] The invention relates to the miniaturization of an electrophoresis system, in particular to an electrophoresis chip for optical detection and an electrophoresis device thereof. Background technique [0002] Capillary electrophoresis is an important separation and detection technique. Capillary electrophoresis combined with sieving media has good separation ability for biological macromolecules, such as DNA and protein, which is one of the important foundations of modern life science research. With the continuous development of life science and other fields, the application demand for capillary electrophoresis is also increasing. Its application range is expanding from purely academic research fields to more practical fields. Correspondingly, the requirements for capillary electrophoresis systems have also expanded from general-purpose and research-oriented to routine or clinical, miniaturized or miniaturized, and dedicated refinement. Capillary elec...

AI Technical Summary

Problems solved by technology

However, incorporating optical fibers on a chip increases the complexity and difficulty of the fabrication process

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