Inactivation method of pathogenic microorganisms of chitosan

A technology of pathogenic microorganisms and chitosan, applied in the field of chitosan inactivation of pathogenic microorganisms and chitosan, can solve the problems of quality influence, molecular weight reduction, poor heat-resistant virus effect, etc., to avoid high-dose irradiation, The effect of maintaining quality and performance

Inactive Publication Date: 2013-06-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But these methods have limitations on the inactivation of chitosan pathogenic microorganisms: the S / D method is only effective for enveloped viruses; the heating method is less effective for heat-resistant viruses , and the experiment found that heating will cause the degradation of chitosan products: chitosan turns from off-white to brown after dry heat sterilization at 170°C for 2 hours; chitosan turns brownish yellow after dry heat sterilization at 120°C for 4 hours, The molecular weight decreased to 27.6% of the initial value; chitosan changed from off-white to yellow after 121°C damp heat sterilization, and the molecular weight decreased to 56.8% of the initial value
γ-ray is an effective method to inactivate pathogenic microorganisms in chitosan, but a higher radiation dose is required to completely inactivate pathogens in chitosan, and the degradation of chitosan is more significant at high doses, which is not quality has some influence
NaOH inactivation of pathogenic microorganisms in chitosan is limited by the two-phase system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1 part of chitosan sample, add about 10 7 cfu / g Bacillus pumilus spores (Spores of Bacillus pumilus), about 10 7 cfu / g Bacillus subtilis spores (Spores of Bacillus subtilis) and about 10 6 TCID 50 / g porcine parvovirus (PPV), 1g / bottle. Add 4.0% NaOH solution, the ratio of the amount of NaOH solution to chitosan is 20:1, keep warm at 80°C, and sterilize until the total number of colonies is less than 1000cfu / g. Filter, wash with water until neutral, subpackage in ampoules, freeze-dry, and the moisture content after drying is 9.6%. Fill with nitrogen gas and seal immediately. 50 kGy gamma-ray irradiation was carried out at -20°C. Reed-muench TCID after irradiation 50 The virus titer reduction (total reduction factor, TRF) was measured by the method, and the reduction of the number of colonies was measured.

[0021] The experimental results show that the residual titer of PPV is ≤0.5 lg TCID after initial inactivation by 4.0% NaOH solution at 80℃ and irradiate...

Embodiment 2

[0023] The preparation of chitosan is with embodiment 1.

[0024] The mass concentration of NaOH solution is 15%, the ratio of NaOH solution to chitosan is 30:1, and sterilized at 60°C until the total number of colonies is less than 1000cfu / g. Filter and wash with water until neutral. Dry under reduced pressure to a moisture content of 8.8%. The ampoules are subpackaged, filled with nitrogen, and sealed. A total dose of 25kGy γ-ray irradiation was carried out at room temperature, and the residual titer of PPV after irradiation was ≤0.5 lg TCID 50 , the number of colonies is reduced to ≤10cfu / g. The crystallinity of chitosan before and after irradiation was 36.4%, 36.7%, the degree of deacetylation were 84.2% and 84.6%, and the molecular weight decreased to 77.8% of the initial molecular weight.

Embodiment 3

[0026] The preparation of chitosan is with embodiment 1.

[0027] The mass concentration of NaOH solution is 40%, the ratio of NaOH solution to chitosan is 50:1, the temperature is room temperature, and the bacteria are sterilized until the total number of colonies is less than 1000cfu / g. Filter and wash with water until neutral. Drying under reduced pressure, the moisture content after drying is 8.9%. The ampoules are subpackaged, filled with nitrogen, and sealed. A total dose of 10kGy γ-ray irradiation was carried out at 30°C, and the residual titer of PPV after irradiation was ≤0.5 lg TCID 50 , the number of colonies is reduced to ≤10cfu / g. The crystallinity of chitosan was 36.4% and 36.2% before and after irradiation, the deacetylation degree was 84.2% and 85.0% respectively, and the molecular weight decreased to 80.1% of the initial molecular weight.

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Abstract

An inactivation method of the pathogenic microorganisms of chitosan belongs to the technical field of the biological material processing. The method comprises the following steps: performing primary inactivation to the pathogenic microorganisms of chitosan with NaOH solution, then drying chitosan, introducing nitrogen, sealing, and performing 10-50kGy of gamma-ray irradiation at -20 to 30 DEG C to inactivate possible pathogenic microorganisms in chitosan and ensure the safety of chitosan used as the biological material. Compared with the existing inactivation method of the pathogenic microorganisms, by using the method of the invention, the pathogenic microorganisms such as bacteria and viruses can be effectively killed, the quality indexes of chitosan such as deacetylation degree and crystallization degree can not change and the reduction of the molecular weight is obviously lower than that of other inactivation methods. Therefore, the quality and function of the biological material can be maintained while the pathogenic microorganisms brought by the possible pollution can be effectively killed.

Description

Technical field [0001] The invention involves chitosan, especially the pathogenic microorganisms of a chitosan, which belongs to the field of biomagonic material processing technology. Background technique [0002] The named polycal chemical name is Jug (1,4) -2-amino-2-deoxida-β-D-polysaccharis, which is mainly extracted from the abolished animal shell and fungal cell walls widely existing.It is a natural amino polysaccharide.Because of its good biocompatibility and biodegradation, shell polysaccharides are widely used in biomaterials, medicine, food and other fields.However, chitosan is derived from the animal and may be contaminated from various pathogenic microorganisms from viruses to bacteria. At the same time, chitosan may also be affected by factors such as instruments and utensils, reagents, and production environments in processing and storage.Pollution of pathogenic microorganisms such as viruses.If these pollutants are spread to people, they will cause serious health ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/08A61L2/18A61L101/02
Inventor 张家骊夏文水李博
Owner JIANGNAN UNIV
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