Gravity drive microfluidic device for preparing monodispersed emulsion and method thereof

Abstract

The invention relates to a gravity drive microfluidic device for preparing a monodispersed emulsion and a method thereof, which is characterized in that a microfluidic system is driven by utilizing the constant flow rate generated by means of the constant gravity action of fluid columns with different heights based on the microfluidic device; liquid drops with different and even volumes are formed by utilizing the action of a shearing force, the surface tension and the like of the continuous phase fluid to the dispersed phase fluid; and microspheres meeting the requirements on bioanalysis and carriers of proteins, genes, medicine screening and the like are formed by solidification, drying or ultraviolet irradiation polymerization. The device of the invention comprises a microfluidic control device support component, a constant sample introduction component, a fluid pipeline, a microfluidic control channel component and a continuous sample adding component, wherein the heights of two-phase liquid columns are automatically controlled by controlling a two-phase mechanical motor; the liquid columns with different heights drive the fluid to generate the constant speed; the continuous-phase fluid for driving the microfluidic control device is used for shearing the dispersed phase liquid to prepare the liquid drops with the different volumes; and the large-scale and continuous preparation of the liquid drops can be realized by the continuous sample adding component and the constant sample introduction component.

Description

technical field [0001] The present invention relates to a gravity-driven microfluidic device that can be used for the preparation of monodisperse emulsions. Based on the microfluidic device, the microfluidic system is driven by the constant flow rate generated by the constant gravity of the fluid column at different heights, and the shear force and surface tension of the continuous phase fluid on the dispersed phase fluid are used to form a uniform size. The droplets are then solidified and dried or polymerized by ultraviolet radiation to form microspheres that meet the carrier requirements for biological analysis and protein, gene, and drug screening. Background technique [0002] With the continuous development of biomedical technology, the liquid phase flow chip technology with microspheres as the carrier has been more and more used in biological analysis and protein, gene and drug screening. Compared with other forms of solid phase carriers, microspheres have significan...

AI Technical Summary

Problems solved by technology

Commonly used polymer microsphere preparation methods include emulsion polymerization, dispersion polymerization, seed polymerization, suspension polymerization and microsuspension polymerization, etc., but the particle size of microspheres prepared by this method is in the nanometer and submicron range, and will Difficulty encapsulating certain particles in polymers

The methods for preparing polymer microspheres with a particle size of several hundred microns to several millimeters include spray drying method and template method, but there are problems such as uneven particle size, high requirements for production equipment, and high production cost.

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