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Ocean source Bacillus barbaricus SCSIO 02429 and method for preparing squid small peptide by using same

A technology for squid and squid viscera is applied in the field of preparing squid small peptides, which can solve the problems of excessive hydrolysis, difficult absorption and utilization of crude protein, low hydrolysis rate of crude protein, etc. The effect of reducing seedling mortality

Active Publication Date: 2011-02-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrolysis method of squid viscera at the present stage still has the problem that the hydrolysis rate of crude protein is low, and the residual crude protein in the enzymatic hydrolyzate is difficult to be absorbed and utilized by marine animals.
However, after increasing the degree of hydrolysis of crude protein by increasing the reaction temperature, prolonging the reaction time, increasing the amount of catalyst, etc., excessive hydrolysis will occur, and the yield of the target small peptide will be greatly reduced. More than 70%, but the yield of small peptides is very low

Method used

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  • Ocean source Bacillus barbaricus SCSIO 02429 and method for preparing squid small peptide by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, and 15 g of agar into 1 L of water, and adjust the pH to 7.0 to obtain a strain activation medium.

[0022] Weigh squid viscera slurry 100g, peptone 25g, yeast extract 25g, compound salt 50g, add deionized water 5000mL, heat and dissolve, adjust pH to 7.0, move into fermenter, steam sterilization, obtain enzyme-producing fermentation medium, wherein The compound salt is composed of 20.9g of sodium chloride, 8.3g of potassium sulfate, 8.3g of magnesium chloride and 12.5g of ammonium chloride.

[0023] The strain of Bacillus barbaricus SCSIO 02429 was transferred to the strain activation medium and cultured at 30°C for 24h. After activation, add the strain into a fermenter with 5L enzyme-producing fermentation medium, and ferment for 24 hours at pH=7.0, 30°C, stirring speed 100r / min, and aeration ratio 0.2. When the protease activity reaches 2000 units / mL, Fermentation was completed when the aminopolysac...

Embodiment 2

[0030] Add 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, and 15 g of agar into 1 L of water, and adjust the pH to 7.0 to obtain a strain activation medium.

[0031] Weigh 150g of squid pulp, 25g of peptone, 25g of yeast extract, 50g of compound salt, add 5000mL of deionized water, heat to dissolve, adjust the pH to 8.0, move into a fermenter, and steam sterilize to obtain an enzyme-producing fermentation medium, in which the compound The salt consisted of 26.6g of sodium chloride, 6.7g of potassium sulfate, 6.7g of magnesium chloride and 10.0g of ammonium chloride.

[0032] The strain of Bacillus barbaricus SCSIO 02429 was transferred to the strain activation medium and cultured at 37°C for 18h. After activation, add the strain into a fermenter with 5L enzyme-producing fermentation medium, and ferment for 12 hours at pH=8.0, 37°C, stirring speed 100r / min, and aeration ratio 0.2. When the protease activity reaches 3000 units / mL, Fermentation was completed whe...

Embodiment 3

[0036] Oni tilapia larvae are provided by Guangdong Baishilian Tilapia Fry Breeding Farm, which is the same batch of Oni tilapia larvae that have been filmed for 1 day. Before the experiment, the larvae were domesticated for 2 days in a 50-liter plastic bucket, and no bait was fed during the domestication period.

[0037] Oni tilapia fry were divided into 1 control group and 4 experimental groups, wherein the feed formula used in the control group was 46% fish meal, 15% malt root, 19% cassava powder, 3% yeast, 1% soybean lecithin, Choline 0.5%, calcium hydrogen phosphate 0.5%, multivitamin 0.4%, combined mineral salt 0.6%, cellulose 8%, soybean oil 1%, sodium alginate 1%, gelatin 4%, the feed formula used in the experimental group 1 was fish meal 41%, the squid small peptide 5% that embodiment 1 obtains, malt root 15%, barley powder 19%, yeast 3%, soybean lecithin 1%, choline 0.5%, calcium hydrogen phosphate 0.5%, multivitamin 0.4%, 0.6% of compound mineral salt, 8% of cellul...

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Abstract

The invention relates to ocean source Bacillus barbaricus SCSIO 02429 CCTCC No: M2010213. The invention also relates to a method for preparing squid small peptide, which is characterized by comprising the following steps of: adding the Bacillus barbaricus SCSIO 02429 into an inducing enzyme-production fermentation medium to ferment to obtain a crude enzyme solution for hydrolysis; then, after crashing squid abdominal organs into pulp, and adding the crude enzyme solution to carry out zymolysis to obtain a crude protein zymolyte with a destroyed glycosyl side chain; adding bromelin to carry out zymolysis; standing the obtained zymolyte; separating a crude adipose layer and a water layer; and drying after removing the crude adipose layer to obtain the squid small peptide. In the invention, the squid small peptide yield can reach 40-46 percent, and the amino acid yield can reach 16-22 percent. The squid small peptide has the actions of reducing the mortality rate of young seaculture animal and increasing the percentage of liveweight growth, can replace the traditional protein sources of fish meal, and the like in feed and is used for functional protein sources, additives, and the like of seaculture compound feed.

Description

technical field [0001] The present invention relates to a bacillus, in particular to a marine bacillus barbaricus SCSIO 02429 strain, and also relates to a method for preparing squid small peptides by using the strain. Background technique [0002] With the rapid development of my country's marine fishing industry, the annual output of squid has reached about 300,000 tons, and it has become one of the important raw materials for aquatic products processing in my country. During the processing of squid, about 15% of visceral waste is produced. These wastes are rich in protein, but they are extremely perishable and difficult to store. The usual treatment method is to bury the waste after the squid oil is extracted. Recently, there have also been reports that the squid viscera were processed into squid slurries and used directly as fish feed. The rate is not high, and it will also pollute the water environment and increase the chance of disease outbreaks in farmed animals. Th...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12R1/07C12N1/20
CPCC12N1/20A23K1/188C12R1/07C12P21/06A23L1/3053C07K14/43504A23K1/1656A23J3/341A23L1/326A23K1/1631A23K10/14A23K20/147A23K50/80A23L17/10A23L33/18C12N1/205C12R2001/07
Inventor 张偲尹浩罗雄明齐振雄田新朋
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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