Composite anesthetic, preparation method thereof, and use thereof in myocardial infarction animal model

Abstract

The invention relates to a composite anesthetic, a preparation method thereof and use thereof in a myocardial infarction animal model. The composite anesthetic consists of aqueous solution of pentobarbital sodium, aqueous solution of chloralic hydras, and aqueous solution of atropine or an anticholinesterase medicament. The invention has the advantages that: 1, by adopting the composite anesthetic solution, particularly the composite anesthetic solution with a proper amount of atropine, the secretion of the respiratory glands can be inhibited effectively, the air passage blockage degree can be lowered and the survival rate of rats can be improved; and 2, the composite anesthetic has the advantages of low price, simple operation, high rat survival rate, high myocardial infarction success rate, reliable effect and the like, can be used as a normal anesthetic for rat myocardial infarction animal models and provides an instructive anesthetic scheme for surgeries on other small animals. The composite anesthetic, the preparation method thereof and the use thereof are applicable in the field of basic medical animal research and related fields.

Description

technical field [0001] The invention relates to the technical field of anesthetics, preparation methods thereof and application in animal models of myocardial infarction, and relates to a compound anesthetic, preparation methods thereof and application in animal models of myocardial infarction. Background technique [0002] Ischemic heart disease - insufficient blood flow to the heart muscle - is the leading cause of death in the world, including most low- and middle-income countries. In the field of cardiac ischemic diseases, the establishment of animal models is widely used in pathogenesis, drug therapy, and cell therapy. According to different research purposes, the selection of animal platforms is also different. Moreover, the successful establishment of animal models is a key factor. As the first step in the experimental study, coronary artery ligation can cause relatively consistent infarction sites, and the left ventricular function and pathophysiological changes are ...

AI Technical Summary

Problems solved by technology

[0003] Some researchers believe that the reasons for the high mortality rate of rats during the establishment of myocardial infarction animal models are as follows: 1. Depth of drug anesthesia: drug anesthesia If the anesthesia is too shallow, the animal will wake up quickly and the ligation operation cannot be completed; if the drug anesthesia is too deep, the rat will not be able to wake up easily, and it will be difficult to get out of the ventilator, which may lead to pulmonary edema, lung infection, and even the risk of death from anesthesia overdose

2. Airway obstruction: bleeding in the incision area after tracheotomy, increased airway secretions during animal anesthesia can block the airway and affect the airway ventilation of animals after recovery of spontaneous breathing

3. Lung injury during the operation: Improper operation of surgical instruments and suture needles during the operation can damage the lung tissue, causing additional damage and increasing the mortality of rats

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