Indigenous probiotic microorganism solid fungicide and preparation method and application thereof

A technology for probiotic microorganisms and solid inoculants, which can be used in microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, etc. The process is simple, the concentration of bacteria liquid cells is high, and the effect of saving labor

Inactive Publication Date: 2010-12-15
兰州市南北两山环境绿化工程指挥部
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: the cost of raw materials used is high, the production process is complicated, and energy cannot be saved.
Its disadvantages are: complex preparation process, long fermentation cycle, high cost and limited application

Method used

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  • Indigenous probiotic microorganism solid fungicide and preparation method and application thereof
  • Indigenous probiotic microorganism solid fungicide and preparation method and application thereof
  • Indigenous probiotic microorganism solid fungicide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Embodiment 1: A kind of indigenous probiotic microorganism solid bacterial agent, it is made up of giant Bacillus megaterium (Bacillus megaterium), jelly-like bacillus (Bacillus mucilaginosus), round brown nitrogen-fixing bacteria (Azotobacter chroococcum) and solid medium, and its three bacterial strains The percentage by weight is 55-65% for Bacillus megaterium, 30-35% for Bacillus jelly-like, and 5-10% for Azotobacter torus. The preservation number of Bacillus megaterium is CGMCC 3770; The preservation number of Bacillus frozenis is CGMCC 3769;

[0029] The solid culture medium comprises 50-80% by weight of dross, 15-30% of corn cob powder, 8-12% of bran, 0.5-0.8% of cornmeal, 0.1-3 % urea, 0.2-4% ammonium sulfate, then add fresh tofu wastewater or vermicelli wastewater or corn starch wastewater or potato starch wastewater to make the water content by weight percentage 45-65%, and adjust the pH to 7.0-7.5 with 1% lime water .

Embodiment 2

[0030] Example 2: see figure 1 , a kind of preparation method of native probiotic microorganism solid microbial agent, comprises the following steps:

[0031] A. Preparation of indigenous probiotic Bacillus megaterium and Bacillus jelly-like agar slant medium and cultivation of strains:

[0032] a. Preparation of agar slant medium: 0.5-2% wort juice, 0.5-2% glucose, 0.2-0.6% peptone, 1-3% agar powder, 80-120ml sterile water, after heating and dissolving, use 1% Adjust the pH value to pH 7.0-7.5 with sodium hydroxide, steam sterilize at 110-114°C for 30-35 minutes, and then distribute it under aseptic conditions in dry and sterilized test tubes of 15×1.5cm and place them on an inclined plane. Ready to use after solidification;

[0033] b. Bacterial strain cultivation: respectively take the mother species agar slant bacteria of Bacillus megaterium and Bacillus jelly-like, and replant them on the above-mentioned prepared agar slant medium under aseptic conditions. -Cultivate f...

Embodiment 3

[0045] Embodiment 3: see figure 1 , a kind of preparation method of native probiotic microorganism solid microbial agent, comprises the following steps:

[0046] A. The preparation of primary strains of native probiotic Bacillus megaterium and Bacillus jelly-like agar slant medium and the cultivation of the strains: the same as in Example 2.

[0047] B. The preparation of the primary strain of the indigenous probiotic Azotobacter rufosa and the cultivation of the strain: the same as in Example 2.

[0048] C Indigenous probiotics Bacillus megaterium, Bacillus jelly-like, Azotobacter rotundum liquid culture medium preparation and liquid bacteria:

[0049] (1) For the cultivation of test tube liquid strains, get 100ml of fresh vermicelli waste water, add (NH 4 ) 2 SO 4 0.1g, H 3 PO 4 0.05ml, after fully dissolved, adjust the pH value to 7.0-7.5, divide into dry test tubes of 15×1.2cm, autoclave at 112°C for 30 minutes, cool to 32°C, inoculate Bacillus megaterium and jelly...

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Abstract

The invention mainly relates to fermentation method of microorganism, in particular to a method for preparing bacillus megaterium, bacillus mucilaginosus and azotobocter chroococcum combined solid fungicide by utilizing waste liquid and waste residue which are produced in food industry and application thereof. An indigenous probiotic microorganism solid fungicide is composed of bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and solid culture medium, wherein the weight percents of the three bacterial strains are 55-65%, 30-35% and 5-10% respectively; the preservation number of bacillus megaterium is CGMCC 3770, the preservation number of bacillus mucilaginosus is CGMCC 3769, and the preservation number of azotobocter chroococcum is CGMCC 3768. The microorganism fungicide is used as crop and plant growth regulator or livestock and poultry feed additive. The invention has the advantages that waste liquid and waste residue which are produced in food industry are taken as raw material, no 'three-waste' pollution is produced in production process, production technology is simple, operation is simple, manpower is saved, energy source is saved, and solid fungicide thereof can be taken as microorganism fertilizer.

Description

Technical field: [0001] The invention mainly relates to a microbial fermentation method, in particular to a method and application for preparing a solid microbial agent of a combination of Bacillus megaterium, Bacillus jelly-like and Azotobacter rotundum by using waste liquid and residue produced in the food industry. Background technique: [0002] The preparation method of traditional microbial bacterial agent, used raw material is glucose, peptone, molasses, beef extract, vitamin, inorganic salt and water and is mixed with liquid culture medium, scrapes bacteria lawn from test tube slant strain and inoculates in liquid culture medium, Prepare liquid bacteria by aerobic or anaerobic culture or inoculate liquid bacteria on solid medium composed of corn flour, bran, soybean flour, seed cake, soybean meal and rice bran, etc., and ferment to prepare solid bacteria agent. Its disadvantages are: the cost of raw materials used is high, the production process is complicated, and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20A01N63/02A01P21/00A23K1/16C02F3/34C05F11/08C12R1/11C12R1/07C12R1/065A23K10/18
Inventor 钟芳孙荣高秦伟志李春杰柴青
Owner 兰州市南北两山环境绿化工程指挥部
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