Culture apparatus special for in-situ culture of amniotic fluid/chorionic cells and application thereof

Abstract

The invention provides a culture apparatus special for in-situ culture of amniotic fluid / chorionic cells, which consists of a culture tank, a slide and a culture cover. The culture tank is provided with a solution feeding hole to facilitate solution feeding and changing operation without influencing the growth of anchorage-dependent cells; and the bottom of the culture tank is provided with two supporting bars which play a role in resisting shock and are convenient to take and use. The amniotic fluid / chorionic cells are directly inoculated on the slide; a plurality of clones can be formed on one slide; and the subsequent analysis is also directly performed in situ of the clones. The culture apparatus effectively solves the problem that the cells are gathered in a concentrated way in the conventional culture bottle method and the conventional culture bottle method cannot identify which cloned cell population the chimera comes from. Because the cloned cells are separated from one another, the cells cloned with abnormal chromosomes and normal chromosomes can be counted; therefore, chromosomal variation of individual cells in isolated culture can be eliminated, the chimeric proportioncan be accurately calculated, and the problem that the chimera is difficult to judge is solved.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to an amniotic fluid / chorionic cell in situ culture dish, in particular to a culture device for providing a sterile environment for amniotic fluid / chorionic cell culture in prenatal diagnosis. Background technique [0002] Amniotic fluid / chorionic cell culture and chromosome preparation and analysis are the most commonly used methods for prenatal diagnosis of fetal congenital malformations and genetic diseases, and are the main means for prenatal diagnosis of chromosomal diseases. Most of the cells in the amniotic fluid are amniotic epithelium and fetal exfoliated epithelium, which come from fetal skin (including sweat glands and sebaceous glands), oral mucosa, part of the digestive tract, urethra, and reproductive tract, and endoderm epithelium from the larynx and trachea. After these cells are shed in the amniotic fluid, whether they can be cultured or grown is determined accor...

AI Technical Summary

Problems solved by technology

Because amniotic fluid cell culture has always had problems such as high technical requirements, great difficulty, long culture time, easy contamination, and few cleavage phases, many units still fail to carry out prenatal diagnosis of amniotic fluid

In the past 10 years, although the culture of amniocytes and the production of chromosomes have been continuously improved around the world, the culture and production of amniocytes are still difficult.

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