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High-sensitivity biological sensor and preparation method thereof

A biosensor and high-sensitivity technology, applied in the field of biosensors, can solve the problems of inability to quantitatively detect the detection process, low sensor detection sensitivity, and long detection time, and achieve the effects of low detectable concentration limit, low price, and rapid response

Inactive Publication Date: 2013-08-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a high-sensitivity biosensor and its preparation method to solve the problems of low detection sensitivity of the above-mentioned sensor, quantitative detection and cumbersome detection process, long detection time, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) At room temperature, 2g polystyrene (polymerization degree is 1600) solid is stirred and dissolved in 18g mass ratio with the rotating speed of 50rpm / min N, N-dimethylformamide and In the mixed solvent of tetrahydrofuran, obtain the polystyrene solution that mass fraction is 10%;

[0025] (2) Under the conditions of room temperature and humidity of 40%, a polystyrene solution with a mass fraction of 10% was input to the spinneret at a flow rate of 4mL / h, and the spinneret was connected to a 20kV power supply for electrospinning prepare fibers;

[0026] (3) the fiber that spins out is deposited on the electrode of quartz crystal microbalance, and the distance between receiving electrode and spinneret is 15cm;

[0027] (4) The fiber deposited on the quartz crystal microbalance electrode is sprayed with a gold film with a vacuum metal sputtering instrument, and the spraying time is 1 min;

[0028] (5) putting the electrode of the quartz crystal microbalance that is c...

Embodiment 2

[0032] (1) At room temperature, 3g of polyaniline (polymerization degree is 300) is stirred and dissolved in 17g of N,N-dimethylformamide with a magnetic stirrer at a speed of 250rpm / min, to obtain a polyaniline with a mass fraction of 15%. Aniline solution;

[0033] (2) Under the conditions of room temperature and humidity of 50%, a polyaniline solution with a mass fraction of 15% was input to the spinneret at a flow rate of 2mL / h, and at the same time, the spinneret was connected to an 18kV power supply for electrospinning preparation fiber;

[0034] (3) The fiber spun is deposited on the electrode of the quartz crystal microbalance, and the distance between the receiving electrode and the spinneret is 10cm;

[0035] (4) The fiber deposited on the quartz crystal microbalance electrode is sprayed with a silver film with a vacuum metal sputtering instrument, and the spraying time is 2min;

[0036] (5) soak 20h in the electrode dithiothreitol of the quartz crystal microbalanc...

Embodiment 3

[0040] (1) At room temperature, 6g of polyethyleneimine (polymerization degree is 500) is stirred and dissolved in 14g of dichloromethane with a magnetic stirrer at a speed of 500rpm / min to obtain polyethyleneimine with a mass fraction of 30%. imine solution;

[0041] (2) Under the condition of room temperature and humidity of 60%, the polyethyleneimine solution with a mass fraction of 30% is input to the spinneret at a flow rate of 6mL / h, and the spinneret is connected to a 60kV power supply for Electrospinning to prepare fibers;

[0042] (3) the fiber that spins out is deposited on the electrode of quartz crystal microbalance, and the distance between receiving electrode and spinneret is 30cm;

[0043] (4) Copper film is sprayed on the fiber deposited on the quartz crystal microbalance electrode with a vacuum metal sputtering apparatus, and the spraying time is 10min;

[0044] (5) put the electrode of the quartz crystal microbalance sprayed with fiber and gold film into gl...

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Abstract

The invention provides a high-sensitivity biological sensor and a preparation method thereof. The high-sensitivity biological sensor comprises a quartz crystal micro-balance, and is characterized in that polymer fibers are deposited on an electrode of the quartz crystal micro-balance, a metal film covers the polymer fibers, a self-assembling material covers the metal film, and the self-assemblingmaterial is connected with a biological sensing material. The preparation method is characterized by comprising the following steps that: dissolving a spinning polymer raw material in solvent to obtain an electro-spinning raw material; electro-spinning the electro-spinning raw material; depositing the spun fibers on an electrode of the quartz crystal micro-balance; spraying a metal film on the fibers; soaking the electrode of the quartz crystal micro-balance into a self-assembling material solution; taking out the electrode of the quartz crystal micro-balance to be dried and to be connected with a biological sensing material so as to obtain the high-sensitivity biological sensor. The high-sensitivity biological sensor has the advantages that the sensitivity is high, and the quantitative detection can be precisely performed.

Description

technical field [0001] The invention relates to a high-sensitivity biosensor and a preparation method thereof, belonging to the field of biosensors. Background technique [0002] Traditional biosensors are cumbersome to detect biological processes, have low sensitivity, and take a long time to detect, making it difficult to adapt to actual needs. At present, kits are commonly used in the market to detect organisms. Although the shortcomings of the above-mentioned sensor detection methods have been overcome to a certain extent, the kit detection method can only detect qualitatively, not quantitatively, and the detection is not accurate. Some domestic and foreign experts and scholars use quartz crystal microbalance as a research platform to quantitatively detect organisms, and adopt various methods to improve detection sensitivity, such as covering sensitive materials on electrodes in the form of solid films to prepare biosensors, but they still cannot overcome the sensitivity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327D06M11/83D06M101/20D06M101/30D06M101/26D06M101/38
Inventor 丁彬孙敏俞建勇孙刚
Owner DONGHUA UNIV
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