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Preparation method of pholiota nameko polysaccharide extract

A technology for extracting polysaccharides from Pleurotus ostreatus, applied in the field of preparation of polysaccharides from Pleurotus ostreatus, can solve the problems of high cost, low extraction rate, unsuitable for large-scale production, etc., achieve low cost, simple operation, and increase protein removal ratio Effect

Active Publication Date: 2012-05-30
天津贝罗尼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Pleurotus ostreatus is widely planted in my country. At present, the polysaccharide extracts of Pleurotus ostreatus are all extracted in a small amount for laboratory experiments. The extraction rate is low and the cost is high. It is not suitable for large-scale production and has not been applied in industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Weigh 100g of dried slippery mushroom, add 20 times (2000ml) hot water at 80°C to soak, and let it stand for 12 hours to fully swell. The beater beats three times at a high speed with an interval of 30 seconds, each time for 2 minutes, to obtain a slippery mushroom slurry.

[0037] (2) Add 0.252 g of trypsin with an activity of 2500 U / mg to the above-mentioned slippery mushroom slurry and incubate at 37° C. for 1 hour. Then add 0.168 g of cellulase with an activity of 1000 U / mg and incubate at 55° C. for 2 hours. Then the temperature was raised to 80° C. for 3 hours, and the first extract and precipitate were collected by filtration. Extract the first precipitation with an equal amount of water under ultrasonic wave at 400W for 30 minutes (every ultrasonic work is 5 seconds, with 1 second interval), and filter to get the second extract and residue. Wash the residue of the second filtration twice, each time with 10 times the amount of water added to the residue. F...

Embodiment 2

[0047] (1) Weigh 100g of dried slippery mushroom, add 20 times (2000ml) hot water at 80°C to soak, and let it sit for 12 hours to fully swell. The beater beats three times at a high speed with an interval of 30 seconds, each time for 2 minutes, to obtain a slippery mushroom slurry.

[0048] (2) Add 0.252 g of trypsin with an activity of 2500 U / mg to the above-mentioned slippery mushroom slurry and incubate at 37° C. for 1 hour. Then add 0.168 g of cellulase with an activity of 1000 U / mg and incubate at 55° C. for 2 hours. Then the temperature was raised to 80° C. for 3 hours, and the first extract and precipitate were collected by filtration. Add an equal amount of water to the first precipitation and extract under ultrasonic wave at 400W for 30 minutes (every ultrasonic work is 5 seconds, with an interval of 1 second), and then filter to get the second extraction solution and residue. Clean the residue filtered for the second time twice, each time with 10 times the amount o...

Embodiment 3

[0055] 1. Pretreatment of macroporous resin

[0056] The new resin must be treated before use to remove a small amount of oligomers, organic matter and harmful ions contained in the resin. During the test, the MG-2 macroporous adsorption resin was eluted with absolute ethanol under reflux until the eluent was evaporated to dryness and there was no residue. The resin washed with absolute ethanol was stored for future use after evaporating the solvent.

[0057] Soak the resin with ethanol-water solution to remove air bubbles and fully swell the resin. MG-2 macroporous adsorption resin is packed in ethanol wet column, continue to flow and wash with absolute ethanol on the column, check the outflowing ethanol from time to time, until it is mixed with water and does not become white turbid. Then wash away ethanol with a large amount of distilled water; then use 0.5% hydrochloric acid solution to flow wash on the column, and then use a large amount of distilled water to flow wash ...

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Abstract

The invention discloses a preparation method of a pholiota nameko polysaccharide extract and provides the preparation method which has the advantages of scale production, simple process and low cost. The preparation method comprises the following steps: soaking a pholiota nameko dry product in hot water, and fully swelling; beating, and obtaining pholiota nameko slurry; adding trypsinase in the pholiota nameko slurry, and keeping the temperature for 1h at the temperature of 37 DEG C; further adding cellulase, and keeping the temperature for 2h at the temperature of 55 DEG C; increasing the temperature to 80 DEG C, keeping the temperature for 3h, collecting first extraction solution, and precipitating; adding the same volume of water in first precipitate, carrying out ultrasonic extractionfor 30min by using ultrasonic waves under the power of 400W, and then collecting second extraction solution and residue; cleaning the residue, filtering liquid which cleans the residue and obtaining third extraction solution; merging the first extraction solution, the second extraction solution and the third extraction solution; carrying out vacuum concentration on the extraction solution after merging to 1 / 6-1 / 10 of the total volume; and adding anhydrous ethanol in the extraction solution after the vacuum concentration, leading the concentration of volume percent of the ethanol in the solution to be 60-80%, carrying out ethanol deposition and obtaining pholiota nameko polysaccharides; and further purifying the pholiota nameko polysaccharides.

Description

technical field [0001] The invention relates to a preparation method of a polysaccharide extract from Pleurotus ostreatus. Background technique [0002] Slide mushroom (Pholiota nameko), also known as pearl mushroom, slide mushroom, Basidiomycetes, Scattered mushrooms, Capletaceae, Phosphorus genus. Slippery mushroom is named for the mucus on the surface of the cap. Slippery mushroom has a delicious taste and high nutritional value, and enjoys a high reputation in the world. Pleurotus ostreatus contains polysaccharide substances that can inhibit tumors, and can prevent the infection of Staphylococcus, Escherichia coli, Klebsiella pneumoniae and Mycobacterium tuberculosis. According to research by Japanese scholars, the active ingredients of polysaccharides in the extract of slippery mushrooms have high anti-cancer activity and enhance the immune function of cells, and have various effects such as lowering blood sugar, adjusting blood pressure, and improving bone hyperplasia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 李海平
Owner 天津贝罗尼生物科技有限公司
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