Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure

A tissue engineering and production method technology, which is applied in the production and mold fields of tissue engineering tubular stents with multi-level pore structure, can solve unseen problems and achieve the effects of small batch difference, low cost and good repeatability

Active Publication Date: 2012-05-23
GUANGZHOU SUN SHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, there is no method and corresponding mold that can be perfectly compatible with these three structure building methods.

Method used

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  • Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure
  • Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure
  • Manufacturing method and mould of tissue engineering tubular scaffold with hierarchical porous structure

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preparation example Construction

[0045] The mold for preparing tissue engineering tubular scaffolds of the present invention has a simple structure, and is easy to manufacture and use; it has wide adaptability to raw materials; Bracket; Secondly, because the outside of the cavity is a circular tube with uniform wall thickness, the mold body is isotropic in cross section, and has high sensitivity to temperature selection, temperature change range and trend, and heat transfer direction. Good adaptability, controllability, and small batch differences; in addition, the internal setting of the orifice plate with a gap at the edge, the material and size parameters of each component of the mold, and the distribution of grooves on the inner cavity for fixing the orifice plate , The distribution of holes and gaps on the orifice plate and the number of orifice plates can be adjusted according to the application requirements, and the scalability is good.

[0046] A method for making a tissue engineering tubular support wi...

Embodiment 1

[0060] a) dissolving L-polylactic acid in tetrahydrofuran to prepare a solution with a mass volume concentration of 20 mg / mL;

[0061] b) Assemble the mold and place it in a temperature environment of 50°C so that there is no temperature difference between the various parts of the mold;

[0062] c) injecting the polymer solution into the mold, cooling to 20°C, and quenching after the polymer solution in the mold cavity becomes gel;

[0063] d) Demoulding, soaking in a large amount of water at 4° C. for 3 days, and freeze-drying to obtain a tubular scaffold of L-polylactic acid tissue engineering with a nanofiber microstructure.

Embodiment 2

[0065] a) dissolving polycaprolactone in ethyl acetate to prepare a polymer solution with a mass volume concentration of 40 mg / mL;

[0066] b) Assemble the mold and place it in a temperature environment of 0°C so that there is no temperature difference between the various parts of the mold;

[0067] c) The volume added to the cavity of the mold is about 2mm 3 The sodium chloride needle crystal porogen;

[0068] d) injecting the polymer solution into the mold, cooling to -90°C, and quenching after the polymer solution in the mold cavity is solid;

[0069] e) Demoulding, high vacuum freeze-drying to remove ethyl acetate, water to filter out the sodium chloride porogen, and freeze-drying again to obtain an internal volume of about 2mm 3 Needle-like Microporous Polycaprolactone Tissue Engineering Scaffolds.

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Abstract

The invention discloses a manufacturing method and a specific mould of a tissue engineering tubular scaffold with hierarchical porous structure. The method comprises the following steps: formulation of solution; assembly of the mould; addition of porogen; heating up and keeping constant temperature; cooling and quenching; removal of the porogen and the solution; and drying. The specific mould used in the manufacturing method comprises a tubular mould body, two ends of the tubular mould body are respectively provided with an feeding port and an exhaust end, the feeding port is provided with a feeding port plug, the exhaust end is provided with an extension section, the extension section is provided with an exhaust hole which is provided with an exhaust hole plug, the mould body is internally provided with at least two pore plates, with the middle parts thereof being provided with fixed pores and the edges thereof being provided with gaps through which materials pass. With the help of the manufacturing method and the mould, the tissue engineering tubular scaffold with complex and precise topological structure can be manufactured conveniently.

Description

technical field [0001] The invention relates to a manufacturing method of a tissue engineering tubular support with a multi-level hole structure and a special mold thereof. Background technique [0002] The topology of tissue-engineered tubular scaffolds is critical to the implementation of tissue-engineering techniques. Tissue engineering scaffolds must have precise macroscopic, submicroscopic and microscopic structures in order to meet application requirements. How to fabricate tissue engineering scaffolds whose structural parameters precisely meet the design requirements is a difficult problem that researchers pay attention to. [0003] Typical processing technologies for polymer porous materials with special structures are as follows: (1) low-pressure injection molding technology; (2) porogen addition-removal method; (3) polymer phase separation principle to make polymer methods for porous materials. [0004] Low-pressure injection molding technology can make the macr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B29C45/78B29C45/34
Inventor 全大萍曾晨光谢高艺朱继翔董鹏
Owner GUANGZHOU SUN SHING BIOTECH CO LTD
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