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Ginsenoside compound in syringa pubescens turca and extraction method and application thereof

An extraction method and compound technology, which are applied to the field of saponin compounds and their preparations in the flower of the flower, can solve the problems of incomplete non-saponin components in effective parts, health threat to operators, low content of active components, and the like, and achieve controllable quality. , less pollution, clear chemical composition effect

Inactive Publication Date: 2010-07-21
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biggest disadvantage of this method is that n-butanol is used as the extraction solvent, which is highly toxic and poses a threat to the health of operators; in addition, n-butanol has a high boiling point, and recovery requires a lot of energy, and the extracted effective parts are incomplete Contains more non-saponin ingredients and lower content of active ingredients

Method used

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  • Ginsenoside compound in syringa pubescens turca and extraction method and application thereof
  • Ginsenoside compound in syringa pubescens turca and extraction method and application thereof

Examples

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Embodiment 1

[0028] Take dried and crushed 1Kg of Qiaoling Flower from Funiu Mountain in Western Henan to 40 meshes, add 12Kg of 50% ethanol to soak overnight, heat and reflux for extraction for 4 hours, add 70% ethanol to the residue for reflux extraction twice, each time for 4 hours, combine the extracts, Ethanol was recovered under reduced pressure to obtain 153 g of extract. The ethanol extract is dissolved with 10% ethanol, filtered, and chromatographically separated with 500 grams of macroporous adsorption resin, with ethanol-water (10%, 20%, 30%, 40%, 50%, 60%, 80% and 95 %) gradient elution, each gradient volume is 2.5BV, and the elution flow rate is 1.5BV / h. Collect 40-50% ethanol elution fractions, concentrate under reduced pressure at 50° C., and obtain 18 grams of dry total saponin powder. The contents of oleuropein, 10-hydroxyoleuropein, (8Z)-ligstroside and echinacoside were respectively 24.1%, 7.2%, 6.5%, 45.2% as measured by high performance liquid chromatography, and the t...

Embodiment 2

[0030] Take 2.5Kg of dried and crushed 50-mesh Qiaoling Flower from Funiu Mountain in Western Henan, add 33Kg of 70% ethanol to soak overnight, heat and reflux for extraction for 4 hours, add 70% ethanol to the residue for reflux extraction twice, each time for 4 hours, and combine the extracts , Ethanol was recovered under reduced pressure to obtain 364g of extract. The ethanol extract is dissolved in 20% ethanol, filtered, and chromatographically separated with 1200 grams of macroporous adsorption resin, with ethanol-water (10%, 20%, 30%, 40%, 50%, 60%, 80% and 95% %) gradient elution, each gradient volume is 2.5BV, and the elution flow rate is 1.5BV / h. Collect 40-50% ethanol elution fractions, concentrate under reduced pressure at 55° C., and obtain 42 grams of dry total saponin powder. The contents of oleuropein, 10-hydroxyoleuropein, (8Z)-ligstroside and echinacoside were respectively 22.1%, 7.2%, 5.5%, 42.2% as measured by high performance liquid chromatography, and the ...

Embodiment 3

[0032] Take dried and crushed 5.0Kg of Qiaoling Flowers from Funiu Mountain in Western Henan to 60 mesh, add 60Kg of 60% ethanol to soak overnight, heat and reflux for extraction for 4 hours, add 90% ethanol to the residue for reflux extraction twice, each time for 4 hours, and combine the extracts , Ethanol was recovered under reduced pressure to obtain 705g of extract. The ethanol extract is dissolved with 30% ethanol, filtered, and chromatographically separated with 2500 grams of macroporous adsorption resin, with ethanol-water (10%, 20%, 30%, 40%, 50%, 60%, 80% and 95% %) gradient elution, each gradient volume is 2.5BV, and the elution flow rate is 1.5BV / h. Collect 40-50% ethanol elution fractions, concentrate under reduced pressure at 60° C., and obtain 85 grams of dry total saponin powder. The contents of oleuropein, 10-hydroxyoleuropein, (8Z)-ligstroside and echinacoside were respectively 20.1%, 6.2%, 5.8%, 43.2% as measured by high performance liquid chromatography, an...

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Abstract

The invention belongs to a technique for extraction separation and application of active ingredients of Chinese medicaments, and particularly relates to a ginsenoside compound in syringa pubescens turca and an extraction method and application thereof. The ginsenoside compound in the syringa pubescens turca is prepared by a method comprising the following steps: crushing dry syringa pubescens turca; using ethanol to perform reflux extraction on the crushed syringa pubescens turca; performing reduced pressure distillation on an extract to obtain an extractum, and reclaiming the ethanol; dissolving the extractum by using solution of the ethanol, and then separating the dissolved extractum by adopting a macroporous absorbent resin; performing gradient elution on an eluant by using an ethanol-water system; and performing purification to obtain the ginsenoside compound in the syringa pubescens turca. The macroporous absorbent resin used in the method can be regenerated and used repeatedly, and the water and the ethanol are used as solvents to reduce pollution, so the method is economical, simple and convenient, is easy for industrialization; and the obtained total saponins has specific chemical components and controllable quality.

Description

(1) Technical field [0001] The invention belongs to extraction, separation and application technology of effective components of traditional Chinese medicine, and in particular relates to a saponin compound in Qiaolinghua and its preparation method and application. (2) Background technology [0002] Qiao Linghua (Syringa pubescens Turca) is a plant of the genus Syringa of Oleaceae, a shrub, also known as hair (leaf) clove, small-leaf clove, produced in Henan, Hebei, Shaanxi and other provinces. Preliminary studies have shown that Qiaolinghua contains triterpenoids, flavonoids and saponins. The current extraction method is to use ethanol to extract, then use organic solvent petroleum ether, petroleum ether-ethyl acetate, and n-butanol to extract respectively, and take n-butanol as the effective part. The biggest disadvantage of this method is that n-butanol is used as the extraction solvent, which is highly toxic and poses a threat to the health of operators; in addition, n-...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P1/16A61K133/00
Inventor 刘普段文录邓瑞雪梁菊尹卫平
Owner HENAN UNIV OF SCI & TECH
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