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Joint detection reagent for bacterial vaginosis

A technology for joint detection and vaginosis, applied in the determination/inspection of microorganisms, methods based on microorganisms, microorganisms, etc., can solve the problems of sensitivity defects, inability to detect proline aminopeptidase indicating BV, and clinical use restrictions

Inactive Publication Date: 2010-06-23
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, for BV caused solely by the increase of Prevotella and Bacteroides, BV cannot be indicated by detecting proline aminopeptidase activity, that is, the method has sensitivity defects in principle
Although the above methods have their own advantages, there are still defects in varying degrees, which are limited in clinical use.

Method used

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  • Joint detection reagent for bacterial vaginosis

Examples

Experimental program
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Effect test

Embodiment 1

[0037]A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 1 g / l, glucose: 45 g / l, citric acid: 1.5 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-p-nitroaniline: 3.5g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 15g / l. Preparation and testing methods are as described above.

Embodiment 2

[0039] A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 2 g / l, glucose: 40 g / l, citric acid: 1.5 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-α-naphthylamine: 3g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 13g / l. Preparation and testing methods are as described above.

Embodiment 3

[0041] A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 1 g / l, glucose: 50 g / l, citric acid: 1 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-α-naphthylamine: 4g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 17g / l. Preparation and testing methods are as described above.

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Abstract

The invention relates to a joint detection reagent for bacterial vaginosis, which comprises a sialidase activity detection reagent and a praline aminopeptidase activity detection reagent, wherein the sialidase activity detection reagent consists of the following substances: 0.5-2.5g / l enzyme substrate A, 30-60g / l carbohydrate and 1-2g / l citric acid; the enzyme substrate A is 5-bromine-4-chlorine-3-indol-N-acetyl-alpha-sialic acid glycosides, nitrobenzene-N-acetyl-alpha-sialic acid glycosides or naphthol-N-acetyl-alpha-sialic acid glycosides; the praline aminopeptidase activity detection reagent consists of the following substances: 2-5g / l enzyme substrate B, 0.1-0.3mol / l Tris-hydrochloric acid buffer solution and 10-20g / l N-glycylglycine; and the enzyme substrate B is L-proline-paranitroaniline, L-proline-alpha-naphthylamine or L-proline-beta-naphthylamine. The reagent is used conveniently, special devices such as a microscope and the like are not required in detection, the detection speed is high, the sensitivity and the specificity are high, BV detection miss caused by merely detecting the activity of one enzyme is effectively avoided, and the reagent is popularized easily.

Description

technical field [0001] The invention relates to a combined detection reagent for bacterial vaginosis, in particular to a reagent for detecting sialidase activity and proline aminopeptidase activity in vaginal secretions. Background technique [0002] Bacterial vaginosis (BV) is one of the most common diseases in obstetrics and gynecology, with a high infection rate and easy recurrence. 50% of women suffering from BV are prone to premature delivery or low birth weight children, and the children they give birth to are also likely to have various sequelae. Accumulating evidence indicates that BV is a risk factor for organizing choriovulitis, amniotic fluid infection, post-cesarean endometritis, and other adverse pregnancy outcomes and pregnancy complications. In addition, in gynecology, BV is mainly related to salpingitis, pelvic inflammatory disease, ectopic pregnancy, infertility, urinary tract infection, postoperative infection and gynecological tumors. [0003] BV was rec...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/34C12R1/35C12R1/01
Inventor 王则宇杨红云付光宇吴学炜李晟磊郑业焕杨静静杨洋郝许峰谢媛媛
Owner AUTOBIO DIAGNOSTICS CO LTD
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