Joint detection reagent for bacterial vaginosis
A technology for joint detection and vaginosis, applied in the determination/inspection of microorganisms, methods based on microorganisms, microorganisms, etc., can solve the problems of sensitivity defects, inability to detect proline aminopeptidase indicating BV, and clinical use restrictions
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Embodiment 1
[0037]A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 1 g / l, glucose: 45 g / l, citric acid: 1.5 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-p-nitroaniline: 3.5g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 15g / l. Preparation and testing methods are as described above.
Embodiment 2
[0039] A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 2 g / l, glucose: 40 g / l, citric acid: 1.5 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-α-naphthylamine: 3g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 13g / l. Preparation and testing methods are as described above.
Embodiment 3
[0041] A combined detection reagent for bacterial vaginosis, comprising a sialidase activity detection reagent and a proline aminopeptidase activity detection reagent; the sialidase activity detection reagent consists of the following substances: 5-bromo-4- Chloro-3-indole-N-acetyl-α-sialoside: 1 g / l, glucose: 50 g / l, citric acid: 1 g / l. The proline aminopeptidase activity detection reagent consists of the following materials: L-proline-α-naphthylamine: 4g / l, Tris-hydrochloric acid buffer solution: 0.2mol / l, N-glycylglycine: 17g / l. Preparation and testing methods are as described above.
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