Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of belt-lactam antibiotics residues in milk

A technology of lactams and detection methods, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators, etc., which can solve the problems of cumbersome operation steps, long detection time, poor selectivity, etc. problem, to achieve the effect of simple operation method, fast detection speed and high accuracy

Inactive Publication Date: 2010-05-12
KUNMING UNIV OF SCI & TECH
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned methods have their own strengths in detection precision, etc., some have low sensitivity and poor selectivity; some operation steps are more cumbersome, and some need to purchase expensive instruments, but most of them have the disadvantages of long detection time and high cost. Processing enterprises cause a lot of inconvenience

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested are mixed with 50 milliliters of acidified acetonitrile respectively, and then centrifuged at 3000 r / min for 3 min. Said acidified acetonitrile is composed of hydrochloric acid: acetonitrile by volume = 1:1 prepared;

[0022] 2. Then use 1% dilute sulfuric acid to adjust the pH value of the centrifuged sample supernatant to 6, add 0.1g / L copper sulfate solution according to the volume ratio of the supernatant to 1:2, shake well and let it stand for 15 minutes to react;

[0023] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 240nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested.

[0024] Record the data and calculate that the content of β-lactam antibiotics in the blank sample is 0.01mg / L, the content of β-lactam antibiotics in the sample to...

Embodiment 2

[0026] 1. 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested were mixed with 200 milliliters of acidified acetonitrile respectively, and then centrifuged at 800 r / min for 10 min. Said acidified acetonitrile was composed of hydrochloric acid: acetonitrile by volume ratio = 1:2 prepared;

[0027] 2. Then use 10% dilute sulfuric acid to adjust the pH value of the centrifuged sample supernatant to 2, add 2g / L lead acetate solution according to the volume ratio of the supernatant to 1:2, shake well, and let it stand for 5 minutes;

[0028] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 380nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested.

[0029] Record the data and calculate that the blank sample β-lactam antibiotic content is 0.85mg / L, the test sample β-lactam antibiotic content is 1.04mg / L, and the y...

Embodiment 3

[0031] 1, 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested were mixed with 100 milliliters of acidified acetonitrile respectively, and then centrifuged at 1500 r / min for 8 min. Said acidified acetonitrile was composed of hydrochloric acid: acetonitrile by volume ratio = 1:5 prepared;

[0032] 2. Use 6% dilute sulfuric acid to adjust the pH value of the supernatant of the centrifuged sample to 3, add 1g / L cobalt chloride solution according to the volume ratio of the supernatant to 1:2, shake well, and let it stand for 8 minutes;

[0033] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 320nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested.

[0034] Record the data and calculate that the blank sample β-lactam antibiotic content is 0.93mg / L, the test sample β-lactam antibiotic content is 1.21mg / L, and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection method of belt-lactam antibiotic residues in milk. The belt-lactam antibiotic residues in milk is detected by the following three steps of centrifugation, chemical reaction of the belt-lactam antibiotic and heavy metal and ultraviolet spectrophotometry; the sample amount and the dosage of various reagents are less in the detection process; the method has less harm on the environment and rapid speed, and the whole operation process can be finished within 30min; and the detection can be finished only in need of equipping a low-speed centrifugal machine and an ultraviolet spectrophotometer without purchasing expensive antibiotic detector.

Description

technical field [0001] The invention relates to a method for detecting residues of beta-lactam antibiotics in milk, in particular to a qualitative and preliminary quantitative method for rapid detection of residues of beta-lactam antibiotics in milk. Background technique [0002] Milk is a liquid food with the most complete nutrients and the highest nutritional value after human breast milk. Milk is rich in protein, fat, amino acid, sugar, salt, calcium, phosphorus, iron and other nutrients, as well as vitamins, enzymes and antibodies, etc. It is most easily digested and absorbed by the human body. However, there are antibiotic problems in milk in actual production. Antibiotics with β-lactam rings such as penicillin G, amoxicillin, ampicillin, cephalosporins, and cloxacillin can be used as efficient veterinary drugs in the dairy industry. To treat diseases such as mastitis, with the improvement of living standards, the problem of antibiotic residues in milk has been paid mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 杨亚玲张丹扬张尹刘谋盛
Owner KUNMING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com