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Penicillenic acid mercaptan mercuric salt and preparing method thereof

A technology of penicramic acid thiol and mercury salt, which is applied in the direction of mercury organic compounds, can solve the problems of poor specificity of mercury ion antigen, poor antigen safety, and low antibody titer, and achieve stable properties and immunogenicity Strong, simple reaction system effect

Inactive Publication Date: 2010-02-24
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some researchers use glutathione as a coupling agent (D.E.Wylie, L.D.Carson, R.Carlson, F.W.Wagner, S.M.Schuster, Anal.Biochem.194 (1991) 381) to connect mercury ions and proteins. This method improves The shortcomings of chelating agents as coupling agents, mercury ions and glutathione thiol groups are covalently linked, and will not dissociate in animals, but there are also disadvantages: glutathione as coupling agents, immunogens The sex is too low, the antibody titer produced is very low
[0005] Therefore, the technical problem to be solved by the present invention is: to overcome the shortcomings of the mercury ion antigen prepared in the prior art, such as poor specificity, poor antigen safety and low immunogenicity, to provide a new antigen and its preparation method

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  • Penicillenic acid mercaptan mercuric salt and preparing method thereof
  • Penicillenic acid mercaptan mercuric salt and preparing method thereof
  • Penicillenic acid mercaptan mercuric salt and preparing method thereof

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Embodiment

[0026] Step 1, the preparation of penicramic acid thiol mercury salt

[0027] Dissolve 50mg of penicillin G sodium salt or potassium salt in 10ml of ultrapure water, add 1g of imidazole, stir to dissolve completely, add 1M HCl drop by drop to adjust the pH to 6.8; HgCl 2 , that is, 7ml of 8mg / ml HgCl 2 The solution was heated in a water bath at 60°C for 2 hours, taken out, cooled at room temperature, and the reaction produced a yellow powdery precipitate with the smell of bitter almonds;

[0028] Step 2, the purification of penicramic acid thiol mercury salt

[0029] Because the penicramic acid thiol mercury salt molecule contains metal, it has the properties of an inorganic substance, and it is insoluble in an acidic solution. Therefore, the penicramic acid thiol mercury salt can be separated and purified by acid precipitation. Add excess 1M HCl to the solution obtained in step 1, shake gently while dripping, a large amount of yellow flocculent precipitates are produced in...

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Abstract

The invention discloses a penicillenic acid mercaptan mercuric salt and a preparing method thereof, belonging to the technical field of chemical industry. The method comprises the following steps to prepare penicillenic acid mercaptan mercuric salt: 1. under the existence of iminazole, causing penicillin G sodium salt or penicillin G potassium salt to react with mercuric chloride; 2. purifying thereaction product to prepare the penicillenic acid mercaptan mercuric salt. The preparing method of the invention has simple reaction system and simple preparing process; the prepared penicillenic acid mercaptan mercuric salt has stable quality and can serve as the hapten of mercuric ions.

Description

technical field [0001] The invention relates to a mercury salt in the technical field of chemical industry and a preparation method thereof, in particular to a mercury penicillothiol salt and a preparation method thereof. Background technique [0002] Heavy metal ions are toxic, persistent environmental pollutants. In the natural environment, especially in water and soil, residual heavy metal ions can enter the human body directly or indirectly, and have an irreversible and strong interaction with proteins or functional enzymes in the human body, posing a serious threat to human health. Therefore, the detection and monitoring of heavy metals in the environment is very necessary. The more commonly used heavy metal detection methods are instrumental analysis methods, such as atomic absorption spectroscopy (atomic absorption spectroscopy, AAS), inductively coupled plasma emission spectrometry (ICP-AES), inductively coupled plasma mass spectrometry (ICP-MS), ion Chromatography...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F3/14
Inventor 曹成喜周培谢鹏席涛樊柳荫张薇
Owner SHANGHAI JIAO TONG UNIV
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