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Natural polypeptides material for biomineralization and preparation method thereof

A technology of natural silk and protein peptides, applied in non-active components of polymer compounds, immobilized on/in organic carriers, medical science, etc., can solve difficult ZnO particles biocompatibility, high cost, artificial polypeptide chain short and other problems to achieve good biocompatibility and bioactivity

Inactive Publication Date: 2010-01-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex preparation process and high cost of this artificial polypeptide, and the relatively short artificial polypeptide chain, it is difficult to make ZnO particles have good biocompatibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] The preparation method of the mineralization silk protein polypeptide material proposed by the present invention mainly comprises the following steps:

[0023] (1) Put silk protein into 10%-25% Na 2 CO 3 The solution is boiled in a beaker for 10 to 30 minutes until the sol-like substance sericin is generated in the solution; when the polypeptide material is prepared by using spider silk protein, this step may not be operated.

[0024] (2) Wash the silk fibroin after degumming in step (1) or the spider silk that has not passed through step (1) with deionized water, add hydrochloric acid with a concentration of 1 mol / liter to 10 mol / liter, Under acidification and hydrolysis reaction for 5 to 24 hours;

[0025] (3) Adjust the pH value of the hydrolyzed solution in step (2) to 7, then place the hydrolyzed polypeptide solution in a dialysis bag, place it in a 4°C refrigerator, and change the water once to three times a day;

[0026] (4) The transparent solution obtained a...

Embodiment 1

[0028] In the prepared 50ml degummed silkworm silk protein solution with a concentration of 2wt%, add 50ml of hydrochloric acid solution with a concentration of 7 mol / liter dropwise, pour it into an ampoule bottle, protect the bottle with nitrogen gas after vacuuming, seal the bottle, and put Keep warm in water at 80°C for 8 hours. The hydrolyzed silk fibroin polypeptide solution was adjusted to pH 7, placed in a dialysis bag, and dialyzed in deionized water for one day. The dialyzed silk fibroin polypeptide solution was centrifuged at 5000rpm for 15 minutes to remove a small amount of precipitate, and the supernatant was taken as the silk protein polypeptide material for mineralization, which was stored in a refrigerator at 4°C for later use. The concentration of the prepared silk fibroin polypeptide solution is 0.1-0.2 mg / mL.

Embodiment 2

[0030] In the 60ml degummed silkworm silk protein solution prepared with a concentration of 3wt%, 60ml of hydrochloric acid solution with a concentration of 5 mol / liter was added dropwise, poured into an ampoule bottle, protected with nitrogen gas after vacuuming, sealed the bottle, and put Keep warm in water at 90°C for 6 hours. The hydrolyzed silk fibroin polypeptide solution was adjusted to pH 7, placed in a dialysis bag, and dialyzed in deionized water for two days. The dialyzed silk fibroin polypeptide solution was centrifuged at 5000rpm for 15 minutes to remove a small amount of precipitate, and the supernatant was taken as the silk protein polypeptide material for mineralization, which was stored in a refrigerator at 4°C for later use. The concentration of the prepared silk fibroin polypeptide solution is 0.2-0.4 mg / mL.

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Abstract

The invention relates to natural polypeptides material for biomineralization and a preparation method thereof, in particular to a natural polypeptides material for preparing a crystal structure with fine biocompatibility by mineralization, belonging to the biological medical material field. The polypeptides material containing natural fibroin consists of degumming fibroin of insect silk and spidroin, which can be used for crystals mineralized at room temperature including zinc oxide, ferric oxide, gallium oxide, cobalt oxide, titanium oxide, hydroxylapatite, calcium phosphate and calcium carbonate. The silk fibroin solution after degumming is subjected to water washing, acid hydrolysis, dialysis, centrifuge enrichment to obtain the natural polypeptides material for mineralization, natural polypeptides material can be used for preparing composite materials with fine biocompatibility by mineralization.

Description

technical field [0001] The invention relates to a natural polypeptide material for biomineralization and a preparation method thereof, in particular to a natural polypeptide material with a good biocompatible crystal structure that can be used for mineralization and belongs to the field of biomedical materials. Background technique [0002] Most spiders and Lepidoptera insects such as silkworms and tussahs can produce a large amount of silk protein. In addition to being used as textile raw materials, these silk proteins have also been developed and utilized in many fields such as medicine, daily chemical industry, and aerospace. In recent years, with the rapid development of molecular biology, people have been able to obtain high-performance, high-yield natural silk proteins through genetic modification. These studies have successfully laid the foundation for the realization of efficient liquid crystal spinning and the development of new biomedical materials in the future. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/60C12N11/02A61K47/42
Inventor 黄忠兵廖晓明严丹红尹光福康云清姚亚东
Owner SICHUAN UNIV
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