Soybean grease extracting method

An extraction method, soybean oil technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of no other nutritional value, complex process, loss of nutritional components, etc., to achieve vegetable oil without solvent residue, high nutritional value, and equipment simple effect

Inactive Publication Date: 2009-12-16
NORTHEAST AGRICULTURAL UNIVERSITY +1
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Problems solved by technology

[0007] It can be seen from the process route that the raw materials were not pretreated, and the oil was extracted through two enzymatic hydrolysis. Due to the limitation of biological enzyme technology at that time, only alkaline protease and neutral protease were used for hydrolysis, and the process Complex, the oil extraction rate can only reach about 65%
Due to the limitations of pretreatment technology, bio-enzyme technology and immature demulsification technology, no relevant papers have been published in China since 1994
In 2000, under the guidance of Professor He Guoqing of Zhejiang University, doctoral candidate Qian Junqing conducted a systematic study on the extraction of soybean oil with aqueous enzyme organic solvents. After the research, the selection of the best enzyme, the best enzymatic hydrolysis process conditions and extraction conditions, and the extraction rate of soybean oil were obtained. reache

Method used

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Examples

Experimental program
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Effect test

experiment example 1

[0057] Experimental example 1 Screening experiment of optimal parameters of soybean protein enzymatic hydrolysis process conditions

[0058] 1 Materials and methods

[0059] 1.1 Materials and reagents

[0060] Soybean Kennong 42 cultivated by Heilongjiang Academy of Agricultural Sciences

[0061] Alcalase alkaline endoprotease Danish novo company

[0062] Flavor protease Danish novo company

[0063] Papain Danish novo company

[0064] Compound protease Danish novo company

[0065] AS1398 neutral protease Danish novo company

[0066] 1.2 Main instruments and equipment

[0067] pHS-25 acidity meter Shanghai Weiye Instrument Factory

[0068] Electronic Analytical Balance Merlot-Toledo Instruments (Shanghai) Co., Ltd.

[0069] Centrifuge Beijing Medical Centrifuge Factory

[0070] Precision Electric Mixer Jiangsu Jintan City Ronghua Instrument Manufacturing Co., Ltd.

[0071] Electric constant temperature water bath Yuyao Dongfang Electrical Instrument Factory

[0072] ...

experiment example 2

[0126] Experimental example 2 Optimal experiment of process parameters in crushing, extruding and puffing pretreatment process of raw materials before enzymatic hydrolysis

[0127] Based on the best water enzymatic extraction process of soybean oil and protein determined in Experimental Example 1, a single factor extrusion test was carried out to determine the parameters of each extrusion expansion system (sleeve temperature, die hole diameter, screw speed, material moisture content) range. Taking the oil extraction rate, protein extraction rate, and total sugar yield in the hydrolyzate as the investigation indicators, a response surface design experiment with 5 factors and 5 levels was carried out

[0128] 2.2 Main instruments and equipment

[0129] pHS-25 acidity meter Shanghai Weiye Instrument Factory

[0130] Electronic Analytical Balance Mellert-Toledo Instruments (Shanghai) Co., Ltd.

[0131] Centrifuge Beijing Medical Centrifuge Factory

[0132] Precision Electric M...

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Abstract

The invention discloses a soybean grease extracting method, which includes steps of: (1) crushing soybean and extruding puffing by a twin screw extruder; (2) mixing the extruded and puffed soybean product with water, adding prolease for enzymolysis; (3) enzyme eliminating, centrifugal separating to obtain the soybean grease. The method uses a method combining the extruding and puffing pre-treatment with biological enzyme to separate soybean grease, and oil of high quality can be obtained by demulsifying emulsible oil prepared by separation with refinery, which is high in nutrient value, and in soybean protein and soybean peptide utilization value. The total oil extraction rate by the method can reach 93.18%. The apparatus for the method is simple in structure, safe for operation, the obtained soybean oil has no solvent residue. Moreover, the method is mist in operation condition, with no reaction between the degradation product in the system and the extract, thus quality of each ingredient is effectively protected. The method is capable of separating oil and protein from the full fat soybean powder at the same time, with degradation rate of protein lowered.

Description

technical field [0001] The invention relates to a method for extracting vegetable oil, in particular to a method for extracting soybean oil, and belongs to the field of extraction and processing of vegetable oil. Background technique [0002] The aqueous enzymatic method uses biological enzymes to hydrolyze soybean raw materials. The oil extraction conditions are mild, and the properties of the oil protein hardly change. Whether it is directly processed and utilized in the water phase or recovered and isolated protein for reuse, the effect is very ideal. However, the method of extracting vegetable oil with water as a solvent is applied to oil plants with low oil content such as soybeans, and the effect is poor, and the oil extraction rate is low. The protein contained in soybean is not only large in quantity, but also excellent in quality. It is a high-quality plant protein. Therefore, it is of great significance to solve the difficult problem of enzymatic extraction of oil ...

Claims

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Application Information

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IPC IPC(8): C11B1/06C11B1/00
Inventor 江连洲李扬吴海波杨柳朱秀清许晶
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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