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Method of producing enterodiol and enterolactone

A technology of enterodiol and enterolactone, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to produce enterodiol and enterolactone on a large scale, environmental pollution, and high cost of chemical synthesis methods problems, to achieve the effects of low production cost, no environmental pollution, and important scientific value

Inactive Publication Date: 2009-10-21
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main way to obtain enterodiol and enterolactone is chemical synthesis method, but the cost of chemical synthesis method is high, and it causes pollution to the environment
[0003] Under strict anaerobic conditions, human intestinal flora can synthesize secoisolariciresinol diglucoside (Secoisolariciresinol, SDG), secoisolariciresinol (Secoisolariciresinol, SECO), modiresinol (Matairesinol, MATA ), syringaresinol (SYRI), pinoresinol (Pinoresinol, PINO) or pinoresinol diglucoside (Pinoresinoldiglucoside, PDG) and other substrates are converted into enterodiol and enterolactone, due to harsh anaerobic conditions and substrate Due to the limitation of the source, it is not possible to use this method to produce enterodiol and enterolactone on a large scale

Method used

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  • Method of producing enterodiol and enterolactone
  • Method of producing enterodiol and enterolactone
  • Method of producing enterodiol and enterolactone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Biotransformation of linseed meal by intestinal bacteria from different human individuals

[0031] 1. Anaerobic medium

[0032] Anaerobic medium (CM605, purchased from Beijing Luqiao Technology Co., Ltd.), its composition is as follows:

[0033] Beef infusion (1000mL); peptone (30g); yeast extract (5g); sodium dihydrogen phosphate (5g); glucose (3g); soluble starch (2g).

[0034] Prepare according to the instructions of the reagent, minced meat slag 5-30g / liter, autoclave at 121°C for 15min.

[0035] 2. Biotransformation

[0036] 1) Take fresh feces from 24 healthy people (see Table 1) and add them to phosphate buffer (pH 7.4) respectively to obtain 24 bacteria solutions with a final concentration of 4g feces / 20mL phosphate buffer.

[0037] 2) Inoculate the 24 kinds of bacterial solutions obtained in step 1 into the anaerobic medium at a ratio of 1:10, and inoculate anaerobically at 37°C (oxygen partial pressure is 3.03975kPa) for 36 hours to enrich the ba...

Embodiment 2

[0045] Example 2: Transformation of linseed meal from human intestinal bacteria liquid

[0046] 1. Prepare culture medium

[0047] 1) Anaerobic medium

[0048] GAM broth medium (Nissui Co., Tokyo, Japan), its composition is as follows:

[0049] 3 grams of soybean peptone; 10 grams of peptone; 13.5 grams of digestive serum powder; 5 grams of yeast extract; 2.2 grams of beef extract; 1.2 grams of bovine liver extract (powder); 3 grams of glucose; KH 2 PO 4 2.5 grams; 5 grams of soluble starch; 0.3 grams of L-cysteine ​​salt; 0.3 grams of sodium thioglycolate; broth (beef heart soup) 1000 ml. Prepare according to reagent instructions. Adjust the pH to 7.2-7.4, autoclave at 10 pounds for 15 minutes.

[0050] 2) Prepare carbon-free medium

[0051] Prepare as follows: add 3g NaCl, 1.0g NH to every liter of phosphate buffer (pH 7.5) 4 Cl, 0.3g L-cysteine ​​hydrochloride; cover with paraffin oil, and autoclave at 121°C for 15min.

[0052] 2. Biotransformation

[0053] 1) Prepa...

Embodiment 3

[0063] Embodiment 3, the impact of the number of passages of human intestinal bacteria on the biotransformation effect

[0064] 1. Prepare culture medium

[0065] 1) Prepare anaerobic medium

[0066] With the step 1 of embodiment 1.

[0067] 2) Prepare carbon-free medium

[0068] Prepare as follows: add 1.5g NaCl, 0.5g NH to every liter of phosphate buffer (pH 7.0) 4 Cl, 0.1g sodium thioglycolate; cover with paraffin oil, and autoclave at 121°C for 15min.

[0069] 2. Strain passage

[0070] 1) Prepare the bacterial liquid of human sample 9 (final concentration is 4g feces / 20ml phosphate buffer), the preparation method is the same as that of step 2 of Example 1, and obtain the bacterial liquid of sample 9.

[0071] 2) Inoculate the bacterial solution of sample 9 into the anaerobic medium at a ratio of 1:10, and perform anaerobic culture at 40° C. (oxygen partial pressure is 7.03975 kPa) for 24 hours to enrich the bacteria.

[0072] 3) Inoculate the enriched bacterial solu...

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Abstract

The invention provides a method of producing enterodiol and / or enterolactone. The method of producing enterodiol and / or enterolactone comprises the steps: using at least one of flaxseed meal, seaweed, sesame meal, rapeseed meal, rye bran, wheat bran, barley bran, corn bran, oat bran and burdock meal as a substrate, carrying out anaerobic culture of the substrate by using intestinal bacteria, and obtaining the enterodiol and / or the enterolactone. The method uses plant resources with low price and intestinal flora of mammals to directly convert the enterodiol and the enterolactone by a biotechnology, and culture fluid enables the contents of the enterodiol and the enterolactone in an extract to be larger than 50 percent (70.15 percent to 91.12 percent) through a simple purification process. The invention has the advantages of simple method, low production cost, no environment pollution, convenient industrial production, important scientific value and high social and economic benefits.

Description

technical field [0001] The present invention relates to a method for producing enterodiol and enterolactone. Background technique [0002] Enterodiol (END) and enterolactone (Enterolactone, ENL) have estrogenic and anti-estrogen-like biological activities, which can prevent breast cancer and prostate cancer. They are internationally recognized phytoestrogens and have been approved by the National Cancer Institute of the United States identified as potential anticancer substances. At present, the main way to obtain enterodiol and enterolactone is chemical synthesis, but the cost of chemical synthesis is high and it causes pollution to the environment. [0003] Under strict anaerobic conditions, human intestinal flora can synthesize secoisolariciresinol diglucoside (Secoisolariciresinol, SDG), secoisolariciresinol (Secoisolariciresinol, SECO), modiresinol (Matairesinol, MATA ), syringaresinol (SYRI), pinoresinol (Pinoresinol, PINO) or pinoresinol diglucoside (Pinoresinoldigl...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12P17/04C12R1/01
Inventor 杨东辉刘树林库宝善
Owner PEKING UNIV
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