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Novel use of salidroside for prevention and cure for osteoporosis

A technology for osteoporosis and salidroside, which is applied in the direction of medical preparations containing active ingredients, bone diseases, organic active ingredients, etc., to increase bone formation and promote proliferation

Active Publication Date: 2009-09-30
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the present invention relates to the role and efficacy of the active ingredient salidroside in Rhodiola rosea in anti-osteoporosis, so far, there are no related reports.

Method used

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  • Novel use of salidroside for prevention and cure for osteoporosis
  • Novel use of salidroside for prevention and cure for osteoporosis
  • Novel use of salidroside for prevention and cure for osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] preparation of salidroside

[0031] Take 500 grams of commercially available Rhodiola rosea extract, add 5000 ml of water to dissolve, heat to 30 ° C, stir and keep warm for 2.5 hours. Filtration gave a clear filtrate. The filtrate was adjusted to pH 3, and flowed through the column of HZ-816 adsorption resin (Shanghai Huazhen Technology Co., Ltd.) at room temperature, and then 1200 ml of 30% ethanol was passed through to collect the effluent. The effluent was concentrated to syrup, added HZ-816 adsorption resin, mixed evenly and dried. According to the ratio of dried adsorbent resin: packed adsorbent resin = 1:6, add the dried resin to the chromatographic column filled with HZ-816 adsorbent resin, and pass through 15% ethanol solution at room temperature for layering. analysis. The chromatographic solution was collected and concentrated to dryness. Add ethanol to dissolve, heat to 50°C, and filter while hot. Concentrate the filtrate, cool to 0°C, crystallize out,...

Embodiment 2

[0032] The effect of salidroside on the bmp-2 expression effect in the model

[0033] Experimental method: A new type of anti-osteoporosis drug screening model (patent publication number: CN1441060A) expressing bone-promoting protein bmp-2 was used to observe salidroside through the steps of model construction, transient cell transfection and fluorescence detection. Effects on bmp-2 expression effects in the model. The operation is as follows:

[0034] Model construction: Take the chloramphenicol acetylase reporter plasmid pCAT4.5X (from Melissa B.Rogers, University of South Florida) containing the promoter region of the mouse bmp-2 gene, cut it with Xbal and Xhol, and take the band The promoter region fragment -3365 to -1658bp was digested with Xbal and Xhol and purified with plasmid pSK (Stratagene, USA) at a ratio of 3:1 (molar ratio) for cohesive end ligation, and then transformed into Escherichia coli DH5α competent cells , pick clones, and obtain recombinant plasmid P...

Embodiment 3

[0052] salidroside up-regulates the mRNA level of bmp-2 in human osteoblasts

[0053] Experimental method: Inoculate MG63 cells (human osteoblast-like cells) in a 6-well plate, about 1×10 6 cells / well, after the cells adhered to the wall, replace with serum-free DMEM, and add salidroside (2 μg / ml) for 24 hours, then use TRIZOL (a commonly used RNA extraction reagent, purchased from Invitrogen) to extract total RNA . Further, RT-PCR was carried out by using the cDNA sequence primers of human bmp-2, and the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was selected as an internal reference. Finally, the RT-PCR product was subjected to agarose gel electrophoresis to detect the target band. The specific operation is as follows:

[0054] 1. Extraction of total RNA

[0055] 1) Add 1ml / well (6-well plate) of TRIZOL to dissolve the cells;

[0056] 2) Incubate the cell lysate at 15-30°C for 5 minutes to completely decompose the nucleosomes;

[0057] 3) Add 0...

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Abstract

The invention relates to novel uses of rhodiola effective ingredient in prevention and cure for osteoporosis. As experiment demonstrates: the compound can obviously upgrade osteotropic protein bmp-2 and mRNA expression in an osteoporosis-resisting medicine screening model, obviously facilitates osteoblast proliferation of human, alkaline phosphatase activity and collagen synthesis and increase osteogenous bone formation, thereby possessing prospect of medicine for prevention and cure for osteoporosis.

Description

Technical field: [0001] The invention relates to a new application of the effective components of rhodiola rosea plant in preventing and treating osteoporosis. Background technique: [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass and destruction of bone microarchitecture, leading to increased bone fragility and susceptibility to fractures. With the aging of the global population, the position of osteoporosis in the world's common and frequently-occurring diseases has gradually risen to the seventh place, with more than 200 million people suffering from osteoporosis. The incidence rate is 58% at the age of 58, and it is almost 100% at the age of 70, especially in postmenopausal women. At present, there are about 90 million osteoporosis patients in my country. [0003] In the process of bone formation, osteoclasts (ocs), whose main function is to remove damaged debris and form bone holes, and osteoblasts (obs), whose main function is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7028A61P19/10
Inventor 王真李电东毛根祥邓洪斌何琪扬张月琴司书毅李晓眠
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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