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Application of 3,5-dimethoxy-4-hydroxy benzaldehyde in ray protection

A technology of hydroxybenzaldehyde and dimethoxy, applied in 3 fields

Active Publication Date: 2009-09-23
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no effective protective drug against α-particle radiation damage

Method used

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  • Application of 3,5-dimethoxy-4-hydroxy benzaldehyde in ray protection
  • Application of 3,5-dimethoxy-4-hydroxy benzaldehyde in ray protection
  • Application of 3,5-dimethoxy-4-hydroxy benzaldehyde in ray protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、3

[0027] Example 1, 3,5-dimethoxy-4-hydroxybenzaldehyde's protective alpha particle radiation damage effect

[0028] 1. Cell pre-culture

[0029] Human normal skin fibroblasts HFS were inoculated in cell culture medium, and then cultured in a 5% carbon dioxide, 37°C constant temperature incubator until the exponential growth phase;

[0030] Wherein, the composition of cell culture medium is as follows: add newborn bovine serum, penicillin and streptomycin to DMEM medium, make the final concentration of newborn bovine serum in culture medium be 10% (volume percentage content), the final concentration of penicillin The final concentration of streptomycin was 100 μg / ml.

[0031] Among them, the composition and concentration of the DMEM medium are exactly the same as the conventional cell culture conditions, and are prepared according to the guidelines of Gibco Company.

[0032] 2. Irradiated Cells

[0033] Set up the following 5 groups of experiments:

[0034] Experimental grou...

Embodiment 2、3

[0052] Embodiment 2,3,5-dimethoxy-4-hydroxybenzaldehyde is to gamma ray irradiation animal (mice) protective effect

[0053] Adult Kunming white mice were used as the experimental objects, half male and half male, weighing 18-21g.

[0054] The Kunming mice used in the experiment were randomly divided into six groups, 8 mice in each group, administered intraperitoneally once 2 hours before 5.5Gy irradiation:

[0055] Experimental group 1 (low dose) (D1): 3,5-dimethoxy-4-hydroxybenzaldehyde was injected intraperitoneally into mice at a dose of 1 mg / kg; irradiated;

[0056] Experimental group 2 (medium dose) (D10): 3,5-dimethoxy-4-hydroxybenzaldehyde was injected intraperitoneally into mice at a dose of 10 mg / kg; irradiated;

[0057] Experimental group 3 (high dose) (D100): Inject 3,5-dimethoxy-4-hydroxybenzaldehyde intraperitoneally into mice at a dose of 100 mg / kg; irradiate;

[0058] Control group 1 (vanillin V100): intraperitoneal injection of vanillin into mice at a dose o...

Embodiment 3、3

[0081] The relevant mechanism of the radioprotective effect of embodiment 3,3,5-dimethoxy-4-hydroxybenzaldehyde

[0082] The human T lymphoblastoid cell line AHH-1 was inoculated in the RPMI1640 medium containing newborn calf serum with a final concentration of 10% (volume percentage) for culture, and when the cells grew to 70%-80% contact density, the Cells are divided into 2 groups:

[0083] The first group: 3,5-dimethoxy-4-hydroxybenzaldehyde was added to the 5 treatments so that the final concentrations in the AHH-1 culture solution were 0 μM, 5 μM, 10 μM, 20 μM, 40 μM, After standing for 2h, give 4Gy 60 Coγ-ray irradiation (irradiation at room temperature, dose rate 2.1Gy / min), after irradiation, let stand for 6h, and collect cells.

[0084] The second group: AHH-1 cells without 3,5-dimethoxy-4-hydroxybenzaldehyde and no irradiation (0Gy) were set as control group 1; 3,5-dimethoxy-4 -Hydroxybenzaldehyde, 3,5-dimethoxy-4-hydroxybenzaldehyde at a final concentration of 1...

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Abstract

The invention discloses a new use of 3,5-dimethoxy-4-hydroxy benzaldehyde which is an application of 3,5-dimethoxy-4-hydroxy benzaldehyde in alpha-particle ray damage protection. Test proves that, in the test of cell culture in vitro and ray irradiation with alpha-particle ray, the cell of 3,5-dimethoxy-4-hydroxy benzaldehyde is added in the culture system; the clone forming rate after irradiation is obviously higher than that of the cell without adding medicants, and the gene mutation rate caused by irradiation is obviously lower than that of the cell without adding medicants. Therefore, 3,5-dimethoxy-4-hydroxy benzaldehyde can reduce gene group DNA damage and gene mutation caused by ray irradiation and has the effect of irradiation protection.

Description

technical field [0001] The present invention relates to a new application of 3,5-dimethoxy-4-hydroxybenzaldehyde. Background technique [0002] Radiation protection drugs are not only important equipment drugs to protect against damage from nuclear weapons, but also key treatment drugs for countering nuclear terrorist attacks and responding to nuclear emergencies, and drugs for protecting astronauts from damage caused by space radiation. Nuclear radiation can damage DNA molecules, cause hematopoietic tissue dysfunction, decrease peripheral blood leukocytes, reduce immune function, and cause cancer in the long run, which is extremely harmful to the body. Alpha particles are high-energy radiation substances, and the compound damage they cause to the body is far greater than that of gamma rays, making it more difficult to prevent and treat. At present, there is no effective protective drug against α-particle radiation damage. Therefore, it is of great significance to find non...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P39/00
Inventor 周平坤徐勤枝王林甄蓓
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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