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Activity enhancer for detoxifying enzyme

A technology for detoxification enzymes and enhancers, which is used in organic active ingredients, animal feed, food preparation, etc.

Inactive Publication Date: 2009-09-09
TAKARA HOLDINGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, UGT is known to be able to facilitate the transport of substrate molecules into bile or blood by enhancing the water solubility of substrate molecules, which leads to detoxification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0085] Preparation Example 1: Preparation of Agarobiose

[0086] Agar (AGAR NOBLE) was suspended in 0.1N HCl to a concentration of 10%, and then heated at 100° C. for 19 minutes. 10 ml of the above sample was loaded onto a water-equilibrated Toyopearl HW40C (manufactured by TOSOH Corporation) column (4.4 cm x 85 cm in volume). Separation was performed by gel filtration chromatography using water as mobile phase at a flow rate of 1.4 ml per minute. The eluted material was detected by differential refractometer and 7 ml fractions were collected.

[0087] Peaks appeared at elution times of 406 minutes, 435 minutes, 471 minutes and 524 minutes, respectively. Take the fractions corresponding to each peak, in silica gel 60 F 254 Plates (manufactured by Merck) were spotted, developed using 1-butanol:ethanol:water at a ratio of 5:5:1, and then analyzed using the orcin-sulfuric acid method. As a result, the peak eluted at 524 minutes was agarobiose. This fraction was lyophilized t...

preparation Embodiment 2

[0088] Preparation Example 2: Preparation of L-glycerol-1,5-epoxy-1αβ,6-dihydroxy-cis-hex-3-en-2-one (DGE)

[0089] A suspension of 2.5 g of commercially available agar (AGAR NOBLE) in 50 ml of 0.1N HCl was heated at 100° C. for 13 minutes to obtain a solution. The solution was cooled to room temperature, the pH was adjusted to 12 using NaOH, and then the solution was neutralized.

[0090] The neutralized product was purified using normal phase HPLC as described below. Each peak was collected, dried under reduced pressure, and redissolved in water. The cancer cell growth inhibitory activity of each fraction was measured by using HL-60 cells. Fractions with retention times of 4.05 and 4.16 were found to have cancer cell growth inhibitory activity.

[0091] Then, fractions with retention times 4.05 and 4.16 were collected in large quantities and subjected to structural analysis. This fraction was found to be L-glycerol-1,5-epoxy-1αβ,6-dihydroxy-cis-hex-3-en-2-one (DGE). Con...

Embodiment 1

[0099] Example 1: Evaluation of the effect of enhancing glutathione S-transferase (GST) activity (1)

[0100] Hepa1c1c7 cells (ATCC CRL-2026) were suspended in Dulbecco's modified Eagle's culture containing 10% fetal bovine serum (manufactured by MP Biomedicals) and 1% penicillin-streptomycin (manufactured by NacalaiTesque Co., Ltd.) base (Dulbecco modified Eagle medium) (manufactured by Sigma), the cell concentration is 4 × 10 5 cells / ml. Into each well of a 96-well microtiter plate, 0.2 ml of cell suspension was added, and cultured overnight at 37° C. in the presence of 5% carbon dioxide gas. Then, replace the medium in each well with Dulbecco's modified Eagle's medium. To each well, 0.4 µl of an aqueous solution of the test substance was added, followed by incubation for 24 hours. The agarobiose obtained in Preparation Example 1 and the commercially available agarooligosaccharide (commercial name: Agaoligo, manufactured by Takara Bio Co., Ltd., both containing 20 to 25% ...

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Abstract

It is intended to provide a drug, a food or a feed which has an effect of enhancing the activity of a second-phase detoxifying enzyme and an effect of increasing intracellular glutathione content.

Description

technical field [0001] The present invention relates to a kind of medicine, food or feed which has the effect of enhancing the second phase detoxification enzyme activity and intracellular glutathione content. Background technique [0002] The food, water, air and chemicals we absorb every day contain ingredients that are not good for living organisms. In living organisms, these components are recognized as foreign objects and then metabolized mainly in the liver. Xenobiotic metabolism in the liver includes a first phase and a second phase. In the first phase, the xenobiotic material undergoes oxidation, reduction or hydrolysis under the action of so-called first-phase detoxification enzymes, such as cytochrome P450s or monooxygenases. In the second phase, in the so-called second-phase detoxification enzymes such as glutathione S-transferase (sometimes referred to hereinafter as "GST"), quinone reductase (sometimes referred to hereinafter as "QR") , UDP-glucuronosyltransf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/729A61K31/351A61K31/7016A61K31/702A23K1/16A23L1/30A61P1/16A61P43/00
Inventor 大野木宏工藤庸子中原宽子榎龙嗣加藤郁之进
Owner TAKARA HOLDINGS
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