Melamine molecular imprinted solid phase extraction cartridge and a preparation method and application thereof

Abstract

The invention discloses a melamine molecular imprinted solid phase extraction cartridge and a preparation method and application thereof. The melamine molecular imprinted solid phase extraction cartridge is characterized in that a glass cartridge provided with a quartz sand sieve plate on the internal bottom is filled with melamine molecular imprinted polymer particles. Melamine, methacrylic acid, ethylene glycol dimethacrylate are mixed and dispersed in the aqueous solution of methanol or ethanol to prepare the melamine molecular imprinted polymer particles which are filled in the glass cartridge and obtain the melamine molecular imprinted solid phase extraction cartridge. The invention achieves the high-efficiency separation, enrichment and purification of melamine in the milk products, compared with the related techniques, such as prior solvent extraction, C18 solid phase extraction and ion exchange solid phase extraction, the melamine molecular imprinted solid phase extraction cartridge has high enrichment and purification efficiency and low cost, can be repeatedly used and is expected to be the necessary means for the residue pre-treatment of melamine in the milk products.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and food safety, in particular to a melamine molecularly imprinted solid-phase extraction column and its preparation method and application, and is mainly used for highly selective separation, enrichment and purification of melamine residues in dairy products. Background technique [0002] Melamine is a triazine nitrogen-containing heterocyclic organic compound, an important nitrogen heterocyclic organic chemical raw material, referred to as triamine, commonly known as melamine and protein essence. Because our country adopts the method of estimating the protein content of food and feed industry, unscrupulous businessmen will mix melamine in food or feed to improve the protein content index in food or feed testing. As a common chemical raw material, melamine is considered to be slightly toxic, but long-term intake of melamine can cause reproductive and urinary system damage, bladder and kidney ...

AI Technical Summary

Problems solved by technology

However, the existing solid-phase extraction cartridges are all disposable and expensive; melamine belongs to strong polar compounds, and in the traditional 18 The retention on the SPE cartridge was poor; while the ion-exchange SPE cartridge could not separate melamine well from the interference of endogenous small molecule components in dairy products

At the same time, due to the complex background of the samples pretreated by the common solid-phase extraction method, it must be combined with gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc., which requires professional operation, and the detection cost is high, so it cannot be popularized in routine detection.