Recombinant rubella virus protein and uses thereof

Abstract

The invention relates to a rubella virus protein for clinical diagnosis of rubella virus infection and a nucleotide sequence encoding the protein. The protein comprises plasmids of the nucleotide sequence and procaryotic host cells. The invention also comprises a method for preparing the protein and application thereof.

Description

technical field [0001] The invention relates to the fields of genetic engineering, vaccine development, diagnostic reagents and the like, in particular to a recombinant rubella virus protein and its application. Background technique [0002] Rubella virus is the only member of the genus Rubellavirus in the Togaviridae family. The spherical rubella virus (RUBELLA VIRUS, RUV) is the pathogen of rubella, and humans are its only host. Rubella is an acute respiratory infectious disease with fever, respiratory tract sputum and rash as the main symptoms. Rubella virus infection in pregnant women, especially in early pregnancy, can lead to fetal congenital rubella syndrome (CONGENITAL RUBELLASYNDROME, CRS). Rubella and CRS occur all over the world, and the infection rate is high in Asia. After pregnant women suffer from rubella, especially within the first 3 to 4 months of pregnancy, rubella virus can invade the placenta and infect the fetus, causing congenital rubella as high as ...

AI Technical Summary

Problems solved by technology

At present, most of the RV protein antigens used in detection kits are derived from virus culture or recombinantly expressed single protein fragments, or due to low purity and poor specificity of protein antigens, false positives are caused and the detection specificity is reduced.

Although the preparation of detection reagents by combining multiple single fragments can avoid the above problems, it will inevitably require multiple extraction and purification processes, and the purification technology requirements for small molecular proteins or peptides are higher

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