Production technique of dual vaccine of chicken new castle disease and infectious bronchitis

Abstract

The invention relates to a method for producing a combined vaccine, in particular to a method for producing a combined vaccine against Newcastle disease and infective bronchitis. The method comprises that: before culture in the same embryo, purity of breeding virus of the Newcastle disease and the infective bronchitis is improved; and during culturing in the same embryo, concentrations of two antigens in the combined vaccine against the Newcastle disease and the infective bronchitis are adjusted to reach reasonable combination. The vaccine prepared by the method is vaccinated with chicken flocks to generate ND and IB immunity without any interference. Furthermore, the vaccine strains are reasonably matched to effectively solve the problem of serious interference in simultaneous vaccination of the Newcastle disease living vaccine and infective bronchitis living vaccine, improve immunization quality and reduce the number of immunization.

Description

technical field [0001] The invention relates to a production method of a dual vaccine, specifically, a production process of a dual vaccine for Newcastle disease and infectious bronchitis, belonging to the field of bioengineering. Background technique [0002] Newcastle disease virus (Newcastledisease virus, NDV) is also known as Asian fowl plague virus, pseudo fowl plague virus or avian pneumoencephalitis virus. The position in virus taxonomy belongs to Paramyxoviridae (Paramyxoviridae), a species in Paramyxovirus genus (Paramyxovirus). The virus mainly harms chickens, guinea fowls and turkeys, and spreads rapidly among the infested flocks, and the virulent strain can destroy the whole flock of chickens. Attenuated strains only cause respiratory tract infection and decreased egg production in chickens, but they can recover quickly. Humans can develop conjunctivitis or lymphadenitis due to contact with sick birds and live virus vaccines, but recover quickly. Newcastle dis...

AI Technical Summary

Problems solved by technology

There are many varieties of multiple vaccines for veterinary use in my country, such as "Newcastle disease, infectious bronchitis dual vaccine", "Newcastle disease, infectious bronchitis, bird flu triple vaccine" and so on. Although there are many varieties, many multiple vaccines It is just a simple combination of multiple single vaccines, the ratio of several antigens is chaotic, the compatibility is not good, and there is a phenomenon of mutual interference of immune responses, which cannot achieve the expected purpose; moreover, the production process of many multiple vaccines is simple and the production technology Rough, causing some products to be polluted, the titer of some antigens in the antigen composition is reduced, and cannot meet the requirements of the production procedures alone

The infectious bronchial virus (IBV) in the "Newcastle disease, infectious bronchitis dual vaccine" interferes with the Newcastle disease virus (NDV). (hereinafter referred to as "Xinzhi Erlian Miao") are basically prepared by using different chicken embryos to culture and proliferate the virus, and then mix the harvested infected allantoic fluid, which will be time-consuming and laborious, and increase the cost, although some Enterprises homeomorphically multiply two viruses, but due to the specific interference of the two viruses, the final antigen production is reduced, and the antigen concentration must be increased through concentration and other processes, which indirectly increases the cost