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Preparation method of gene coatings of medical device

A medical device and gene technology, applied in the field of preparation of gene coatings, can solve the problems of increasing matrix inflammation and restenosis, achieve the effect of avoiding inflammation and restenosis, meeting clinical needs, and simple operation

Active Publication Date: 2009-07-29
LEPU MEDICAL TECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of the matrix, the chances of inflammation and restenosis caused by the matrix are increased

Method used

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  • Preparation method of gene coatings of medical device
  • Preparation method of gene coatings of medical device
  • Preparation method of gene coatings of medical device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of embodiment 1 iNOS gene coating nanopore support

[0053] After the 316L stainless steel alloy is polished, it is laser-engraved into a vascular stent, and then through the combination of acid corrosion and anodic oxidation, the drug-loading holes are directly prepared on the local surface of the raw material of the device body, and the diameter of the holes is 200-300nm.

[0054] Then make the gene or oligonucleotide coating bioscaffold according to the following steps:

[0055] 1. Cleaning of the surface of the bracket body:

[0056] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0057] (2) Use ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water in sequence. The ultrasonic frequency is 100KHz, and the cleaning time is 10 minutes;

[0058] (3) Soak the stent body in a 30g / L, 80°C sodium hydroxi...

Embodiment 2

[0067] Preparation of iNOS gene and rapamycin co-coated bioscaffold with nanopores

[0068] The cobalt-chromium alloy material is selected and polished, and then laser-engraved into a vascular stent; then, through the combination of acid corrosion, micro-arc oxidation and micro-arc nitriding, drug-loading holes are prepared on the surface of the stent, and the holes are drug-loading grooves. The diameter of the hole is 100-200nm. The gene-coated bioscaffold was prepared according to the following steps:

[0069] 1. Cleaning of the surface of the bracket body:

[0070] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0071] (2) Use ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water in sequence. The ultrasonic frequency is 60KHz, and the cleaning time is 15 minutes;

[0072](3) Soak the stent body in a 60g / L, 70°C sodium ...

Embodiment 3

[0082] Preparation of iNOS gene and rapamycin co-coated bioscaffold with nanopores

[0083] After the nickel-chromium alloy material is polished, it is carved into a vascular stent with a laser; then, through the combination of acid corrosion, micro-arc oxidation and micro-arc nitriding, drug-loading holes are prepared on the surface of the stent, and the holes are drug-loading grooves. The diameter of the hole is 150-300nm. The gene-coated bioscaffold was prepared according to the following steps:

[0084] 1. Cleaning of the surface of the bracket body:

[0085] (1) Grinding the surface of the bracket body with an abrasive belt, mechanically removing oxides on the surface, and adjusting the surface roughness;

[0086] (2) Use ultrasonic waves to clean with 75% medical ethanol solution, 99.5% acetone solution, and deionized water in sequence. The ultrasonic frequency is 28KHz, and the cleaning time is 15 minutes;

[0087] (3) Soak the stent body in a 30g / L, 100°C sodium hyd...

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Abstract

The invention relates to a method for preparing a gene-coated medical apparatus. The method utilizes the anode polarization principle, uses a medical apparatus body as an anode, and places the anode and the cathode of a power supply into solution of gene or oligonucleotide for anode polarization reaction so as to obtain the medical apparatus of which the surface carries a gene coating. The method is simple and feasible, does not need to introduce matrix loaded with the gene, and avoids inflammation and side effects brought by the substrate; the apparatus body is evenly coated with the gene or the oligonucleotide, the gene or the oligonucleotide coating has good stability, the activity is not influenced by a technical process, and the gene or the oligonucleotide is easily and slowly released into vascular cells so as to express in vascular wall cells; and the medical apparatus can prevent and treat inflammation reaction and restenosis in the medical apparatus more effectively.

Description

technical field [0001] The invention relates to a medical device, in particular to a method for preparing a gene coating on a medical device. Background technique [0002] Atherosclerosis causes the stenosis of the blood vessel lumen, which leads to the obstacle of blood supply and oxygen supply to the muscle and causes muscle ischemia and necrosis in the corresponding part. In 1976, German scholar Andrea Gronzgo first proposed the idea of ​​installing stents in arteries. By the 1990s, coronary stents had been widely used as a medical device for clinical treatment. [0003] Currently commonly used vascular stents can be divided into bare stents, drug-eluting stents, polymer-coated stents, metal-coated stents, radioactive stents and artificial blood vessel-covered stents according to the surface state. The restenosis rate is small, people have developed radioactive stents and drug-eluting stents, wherein the drugs are various therapeutic agents, etc., and now drug-eluting st...

Claims

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Application Information

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IPC IPC(8): A61F2/82A61F2/06A61F2/00
Inventor 陈永强余占江张正才蒲忠杰王长青冉玉凤
Owner LEPU MEDICAL TECH (BEIJING) CO LTD
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