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Fructose and oligo-fructose production method using garlic fructosan hydrolase

A fructooligosaccharide and hydrolase technology, which is applied in application, food preparation, food science and other directions, can solve the problem of high production cost, and achieve the effect of reducing the cost of product raw materials, reducing environmental pollution, and reducing equipment investment.

Inactive Publication Date: 2009-06-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application number is 200510120909.1 Chinese patent discloses a method for producing garlic fructooligosaccharides by acid hydrolysis, but this production method needs to add edible acid, and the production cost is relatively high

Method used

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  • Fructose and oligo-fructose production method using garlic fructosan hydrolase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] With fresh garlic as raw material, such as figure 1 Shown technological process, specifically carry out according to the following steps:

[0027] (1) Make garlic paste from raw garlic: put 10 kg of fresh garlic cloves in 25 kg of drinking water, soak for 4 hours, rinse with running water to remove impurities such as soil and garlic skin; mix peeled garlic cloves with 5-25 kg Add water to the beater together to make garlic pulp of 100-150 mesh;

[0028] (2) Preparation of fructose and fructo-oligosaccharide extracts: adjust pH=4 with citric acid, etc., and react for 3 hours at 30° C.; filter the processed solution after the reaction with garlic pulp through a 250-mesh filter cloth to obtain a crude extract;

[0029] (3) Preparation of fructose and fructo-oligosaccharide refining extract: the crude extract obtained in step (2) was centrifuged at 4000rpm for more than 20 minutes, and the gained supernatant was filtered through an inorganic membrane with a molecular weigh...

Embodiment 2

[0034] With fresh garlic as raw material, such as figure 1 Shown technological process, specifically carry out according to the following steps:

[0035] (1) Make garlic paste from raw garlic: put 10 kg of fresh garlic cloves in 50 kg of drinking water, soak for 8 hours, rinse with running water to remove impurities such as soil and garlic skin; mix peeled garlic cloves with 5-25 kg Add water to the beater together to make garlic pulp of 100-150 mesh;

[0036] (2) Preparation of fructose and fructo-oligosaccharide extracts: adjust pH=6 with citric acid, etc., and react for 2.5 hours at 20° C.; filter garlic pulp with a bag filter to obtain a crude extract;

[0037](3) Preparation of fructose and fructo-oligosaccharide refining extract: the crude extract obtained in step (2) was centrifuged at 3000rpm for more than 20 minutes, and the obtained supernatant was filtered by a plate and frame press with a pressure of 3MPa, and the permeate It is a transparent refined extract of f...

Embodiment 3

[0042] With dehydrated garlic slices as raw material, proceed as follows:

[0043] (1) Make garlic pulp from raw garlic: soak dehydrated garlic slices in 10 times drinking water for 1 hour; use a beater to make garlic pulp with 100-150 mesh, and add 5 kg fresh garlic paste;

[0044] (2) Preparation of fructose and fructo-oligosaccharide extract: adjust pH=6 with citric acid etc., adjust temperature at 50° C. and react for 0.5 hour; filter garlic pulp with 250-mesh filter cloth to obtain crude extract;

[0045] (3) Preparation of fructose and fructo-oligosaccharide refining extract: the crude extract obtained in step (2) was centrifuged at 4000rpm for more than 20 minutes, and the resulting supernatant was filtered through an inorganic membrane with a molecular weight cut-off of 50,000 Daltons. The liquid is a transparent fructose and fructooligosaccharide solution;

[0046] (4) Separation of fructose and fructo-oligosaccharides: 5 kilograms of cation exchange resins are proc...

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Abstract

The present invention discloses a method taking fresh garlic, dehydrated garlic slice, garlic residue after extracting garlic oil extract and waste water produced in the garlic slice producing process as raw material, using the function of the garlic fructan hydrolase to produce garlic fructose and oligofructose. The method includes the following steps: (1) making the garlic and the like raw material into garlic pulp; (2) preparing the fructose and oligofructose extract; (3) refining the fructose and oligofructose extract; (4) separating the fructose and oligofructose extract; (5) obtaining the oligofructose; (6) obtaining the fructose. The invention uses fructan hydrolase of the garlic raw material itself, thereby reducing the cost increase of adding exogenous enzyme or edible acid; performs hydrolytic decomposition under normal temperature and pressure and equivalent temperature condition, thereby greatly saving energy sources, reducing apparatus investment, and lowering material cost of products; and uses the garlic residue, and waste water produced in the garlic slice producing process as raw materials, thereby greatly improving the raw material use ratio and reducing environmental pollution.

Description

technical field [0001] The invention relates to a method of producing garlic fructose and garlic by using fresh garlic, dehydrated garlic slices, garlic residue after extracting garlic essential oil (volatile oil) or waste liquid produced in the process of producing garlic slices, etc., by utilizing the action of garlic fructanase A method for fructooligosaccharides belongs to the field of extraction, separation and modification of natural products. Background technique [0002] Garlic (Allium sativum L.) is an important medicinal and edible raw material in my country. Traditional medicine often uses it to treat diarrhea, whooping cough, cold cough, athlete's foot and other diseases; modern medicine believes that garlic has the functions of lowering blood fat, preventing arteriosclerosis, and preventing Coronary heart disease, cerebral thrombosis, anti-inflammatory, anti-cancer, improve immunity, protect the liver, lower blood pressure, lower blood fat, and delay aging. Thes...

Claims

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Application Information

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IPC IPC(8): A23L1/09A23L1/29A23L29/30
Inventor 黄雪松
Owner JINAN UNIVERSITY
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