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Preparation of microprotein

A protein and microorganism technology, applied in the field of microbial protein preparation, can solve the problems of unsatisfactory product quality and yield, and achieve the effects of easy industrial production, low cost and high yield

Inactive Publication Date: 2009-05-27
天津市裕川置业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its process is to mix and stir the remaining activated sludge, water, and quicklime at a ratio of 1:1:0.06 for 20-30 minutes; pump the mixed solution into a hydrolysis reactor for hydrolysis, and the working pressure is 0.4-0.7MPa. Under the condition of temperature of 135°C-150°C, the hydrolysis time is 1-3h; after hydrolysis, filter, adjust the pH value of the filtrate to 7.0-8.5 with hydrochloric acid, and then concentrate to become a protein concentrate, which can also be dried to become a dry protein product. The invention utilizes surplus activated sludge to prepare hydrolyzed protein, which has a wide range of sources, low production cost, waste utilization, and environmental protection, but the product quality and yield are not satisfactory

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] A method for preparing protein from activated sludge, comprising the steps of:

[0018] (1) 30kg of activated sludge with a water content of 80% is added to the hydrolysis reactor,

[0019] (2) adjusting pH with water and calcium oxide solution is 10, and water content is 92%, and reaction temperature is controlled at 140 ℃, and reaction time is 3 hours,

[0020] (3) Separating the hydrolyzate with a centrifuge, collecting 54 kg of filtrate, and then three-effect evaporation after membrane filtration to obtain 8 kg of protein solution with a concentration of 5%.

Embodiment 2

[0022] A method for preparing protein from activated sludge, comprising the steps of:

[0023] (1) 23kg of activated sludge with a water content of 80% is added to the hydrolysis reactor,

[0024] (2) adjusting pH with water and calcium oxide solution is 12, and water content is 94%, and reaction temperature is controlled at 120 ℃, and reaction time is 4 hours,

[0025] (3) Separate the hydrolyzate with a centrifuge, collect 62 kg of the filtrate, first filter it with a membrane and then evaporate it with a scraper film to obtain 1.7 kg of a protein solution with a concentration of 20%.

Embodiment 3

[0027] A method for preparing protein from activated sludge, comprising the steps of:

[0028] (1) 15kg of activated sludge with a water content of 80% is added to the hydrolysis reactor,

[0029] (2) adjusting pH with water and sodium hydroxide solution is 13, and water content is 96%, and reaction temperature is controlled at 110 ℃, and reaction time is 2 hours,

[0030] (3) separate hydrolyzate with plate and frame filter press, collect filtrate 66kg, after the pH value of filtrate is adjusted to 6.8 with hydrochloric acid solution, three-effect evaporation obtains the protein solution 0.6kg of concentration 30%,

[0031] (4) The above-mentioned protein concentrate product is dried to obtain 0.15 kg of protein dry product.

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Abstract

The invention relates to a method for preparing microprotein, which takes active sludge generated during biochemical treatment of sewage and waste water, particularly remained active sludge from town sewage treatment plants, as raw materials. The method is characterized in that the active sludge is subjected to concentration regulation, hot basic hydrolysis, residue-liquid separation, filtrate condensation and concentrated solution drying to obtain protein products with different concentrations. The method has the advantages of simple technological process and equipment and easy industrialized production, and protein products prepared have the advantages of good foamability and foaming stability, high yield and low cost.

Description

technical field [0001] The invention relates to a method for preparing microbial protein. The raw material is activated sludge produced in the biochemical treatment process of sewage and wastewater, especially the remaining activated sludge of urban sewage treatment plants. Background technique [0002] Animal protein and vegetable protein are widely used and easily biodegradable. Their usage has increased sharply in recent years, but they are difficult to promote and use in some industries because of their high price. The main dry matter of activated sludge is protein-rich microbial metabolites, and the microbial protein prepared from it can partially replace animal protein and vegetable protein. Chinese patent application 02116227.1 discloses a method for treating activated sludge produced by sewage biochemical treatment and extracting protein. The activated sludge is added to an enamel or stainless steel container, stirred at a temperature of 15-90 degrees Celsius, and ke...

Claims

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Application Information

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IPC IPC(8): C07K1/12C07K1/36
Inventor 刘成林
Owner 天津市裕川置业集团有限公司
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