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Preparation method of tobacco extractum and use thereof

A technology of tobacco extract and tobacco powder, which is applied in the field of preparation of tobacco extract, can solve the problems of high cost and high difficulty in industrial production, and achieve the effects of low production cost, low price and high purity

Active Publication Date: 2009-04-29
广州华芳烟用香精有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In addition, from the perspective of known technologies, the strains used to ferment tobacco with microorganisms are all separated and purified from tobacco or other substances. The workload and difficulty of separation, screening and purification are high, and the cost of industrial production is high.

Method used

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  • Preparation method of tobacco extractum and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Weigh 30g of tobacco powder into a 500mL triangular flask, add distilled water at a solid-to-liquid ratio of 1:5, wrap the mouth of the bottle with gauze, and sterilize and cook for 30 minutes in steam at 100°C in a sterilizing pot.

[0028] Adjust the pH value to 6.0, respectively add cellulase with a weight of 2‰ of tobacco dust and liquefaction enzyme with a weight of 5‰ of tobacco dust, and react in a constant temperature water bath shaker at 50°C and 150r / min for 4h.

[0029] Adjust the pH value to 4.5, add 1% glucoamylase by the weight of tobacco powder, and react at 50° C., 150 r / min in a constant temperature water bath shaker for 3 hours.

[0030] Inject the corresponding strain seed solution into the enzymatic hydrolyzate after the enzyme treatment in proportion, and cultivate it in a constant temperature shaker at 30°C and 120r / min for 3 days. (Lactobacillus delbrueckii inoculation amount 1%; Rhizopus inoculum amount 3%, Aroma yeast inoculum amount 1%)

[003...

Embodiment 2

[0036] Weigh 30g of tobacco powder into a 500mL triangular flask, add distilled water at a solid-to-liquid ratio of 1:7, wrap the mouth of the bottle with gauze, and sterilize and cook in steam at 100°C for 40 minutes in a sterilizing pot.

[0037] Adjust the pH value to 7.0, respectively add cellulase with a weight of 5‰ of tobacco dust and liquefaction enzyme with a weight of 3‰ of tobacco dust, and react in a constant temperature water bath shaker at 60°C and 150r / min for 2h.

[0038] Adjust the pH value to 8.0, add 2% glucoamylase by weight of tobacco powder, and react at 70°C and 150r / min constant temperature water bath shaker for 1.5h.

[0039] Inject the corresponding strain seed solution into the enzymatic hydrolyzate after the enzyme treatment in proportion, and cultivate it in a constant temperature shaker at 50°C and 120r / min for 3 days. (Lactobacillus delbrueckii inoculum 2%; Rhizopus inoculum 1%, Aroma yeast inoculum 2%)

[0040] Take out the fermentation broth a...

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Abstract

The invention discloses an extraction method for a tobacco extract and applications thereof. The invention is characterized in that the tobacco extract is produced by the following steps: step A, tobacco powder is sterilized by steam; step B, an external complex enzyme is accessed for zymohydrolysis; step C, a compound strain is accessed for fermentation; and step D, ethanol is used for extracting, filtering and condensing for obtaining the tobacco extract. In the invention, the complex enzyme is used for processing and the technique of microbial fermentation is combined; therefore, the production condition is moderate, the efficiency is high and the pollution is small; the strain is the commercialized strain commonly seen in the food industry; the purity is high and the price is low; and the tobacco extract is easy to be obtained and the fermentation condition is easy to be grasped and controlled. The tobacco extract obtained by the invention has excellent stability and repeatability; moreover, the tobacco extract has more remarkable fragrant increasing effect and specific style characteristics. The method is simple in production technique, low in production cost is easy to realize commercial production.

Description

Technical field: [0001] The invention relates to the extraction and application of natural spices, in particular to a preparation method and application of tobacco extract. Background technique: [0002] In today's world, the anti-smoking wave is sweeping the world, and the issue of smoking and health has attracted more and more attention. However, as a special consumer product, tobacco products have historical roots and a large consumer group, and it is difficult to disappear from people's lives in a short period of time. Therefore, the development of low-tar, low-harm cigarettes has become the consensus of tobacco manufacturers all over the world. . At present, as far as technical means are concerned, it is no longer a problem to simply reduce cigarette tar, but with the reduction of tar, the aroma components in smoke also decrease, and the satisfaction of smoking decreases, causing smokers to pursue satisfaction. Smoke more. Therefore, low-tar cigarette aroma compensat...

Claims

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Application Information

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IPC IPC(8): A24B15/18A24B15/20A24B15/26A24B3/12
Inventor 赵世兴黎志坚王学娟
Owner 广州华芳烟用香精有限公司
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