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Vitamin A content detection method by high performance liquid chromatography

A high-performance liquid chromatography and vitamin technology, which is applied in the field of determination of vitamin A content by high-performance liquid chromatography, can solve the problems of inability to determine vitamin A content, large interference, and reaching below 1.4.

Inactive Publication Date: 2009-03-25
HANGZHOU MINSHENG HEALTHCARE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The method for determining the content of vitamin A in preparations is usually UV-visible spectrophotometry, which is described in detail in the 2005 edition of the Chinese Pharmacopoeia. This method is cumbersome to operate and has a scope of application. Unable to measure (large interference, non-specific method)
When the presently disclosed reverse-phase high-performance liquid chromatography (mobile phase methanol: water is 90-100: 10-0) measures the content of vitamin A in some compound vitamin solid preparations containing vitamin A, the specificity is poor, and vitamin A The separation between the peak and other impurity peaks can only reach below 1.4, which is difficult to meet the requirements of the Chinese Pharmacopoeia (the separation should be greater than 1.5)

Method used

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  • Vitamin A content detection method by high performance liquid chromatography
  • Vitamin A content detection method by high performance liquid chromatography
  • Vitamin A content detection method by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Measured according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 Edition Two Appendix VA), and operated in the dark.

[0069] Vitamin A Use octadecyl silane bonded silica gel as filler, methanol-water (85:15) as mobile phase, detection wavelength at 325nm, flow rate 1.0ml / min, and the number of theoretical plates calculated based on the vitamin A peak should not be less than 1000, the resolution of the main peak and the miscellaneous peak should meet the requirements.

[0070] Preparation of vitamin A reference substance solution Take an appropriate amount of vitamin A acetate reference substance, accurately weigh it, and prepare a solution with a concentration of about 9 μg vitamin A acetate per 1 ml with isopropanol, and shake it well.

[0071] Preparation of test solution: Take 30 tablets of this product, accurately weigh, grind, and accurately weigh an appropriate amount (approximately equivalent to 7500IU of vitamin A in the preparatio...

Embodiment 2

[0075] Example 2 Measured according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 Edition Two Appendix VA), and operated in the dark.

[0076] Vitamin A Use octadecyl silane bonded silica gel as filler, methanol-water (80:20) as mobile phase, detection wavelength at 325nm, flow rate 1.0ml / min, and the number of theoretical plates calculated based on the vitamin A peak should not be less than 1000, the resolution of the main peak and the miscellaneous peak should meet the requirements.

[0077] Preparation of vitamin A reference substance solution Take an appropriate amount of vitamin A acetate reference substance, accurately weigh it, and prepare a solution with a concentration of about 9 μg vitamin A acetate per 1 ml with isopropanol, and shake it well.

[0078] Preparation of test solution: Take 30 tablets of this product, accurately weigh, grind, and accurately weigh an appropriate amount (approximately equivalent to 7500IU of vitamin A in the preparatio...

Embodiment 3

[0082] Example 3 Measured according to high performance liquid chromatography (Chinese Pharmacopoeia 2005 Edition Two Appendix VA), and operated in the dark.

[0083] Vitamin A uses octadecyl silane bonded silica gel as filler, methanol-water (89:11) as mobile phase, detection wavelength is 325nm, flow rate is 1.0ml / min, and the number of theoretical plates should not be less than calculated by vitamin A peak 1000, the resolution of the main peak and the miscellaneous peak should meet the requirements.

[0084] Preparation of vitamin A reference substance solution Take an appropriate amount of vitamin A acetate reference substance, accurately weigh it, and prepare a solution with a concentration of about 9 μg vitamin A acetate per 1 ml with isopropanol, and shake it well.

[0085] Preparation of test solution: Take 30 tablets of this product, accurately weigh, grind, and accurately weigh an appropriate amount (approximately equivalent to 7500IU of vitamin A in the preparation), pu...

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Abstract

The invention relates to a method for mensurating the content of vitamin A in a high performance liquid phase chromatography method. The method has the following chromatographic conditions: a. a filling agent for a chromatographic column is octadecyl silane bonded silica gel; b. a chromatographic mobile phase is a mixed solution of methanol and water in a mixed proportion of 80-90 to 20-10 (excluding 90 to 10); and c. the detection wavelength of an ultraviolet detector is 325 nm. The flow speed of the mobile phase is 1 ml / min; and the size of sample introduction of the mobile phase is 20 mu l. The method is accurate and reliable; the linear range is between 36.72 and 68.85mu g / ml; a linear related coefficient r is equal to 0.9992; the reclaiming rate is equal to 100.3 percent; and RSD is less than 2 percent.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for quantitatively determining the content of vitamin A in preparations, in particular to a related technology for determining the content of vitamin A by high performance liquid chromatography. Background technique [0002] The method for determining the content of vitamin A in preparations is usually ultraviolet-visible spectrophotometry. The Chinese Pharmacopoeia 2005 has a detailed description of it. This method is cumbersome to operate and has a scope of application. The content of vitamin A in some preparations is still Can not be measured (the interference is large, the method is not specific). The published reversed-phase high performance liquid chromatography (mobile phase methanol: water 90-100:10-0) is used to determine the content of vitamin A in some compound vitamin solid preparations containing vitamin A. The specificity of vitamin A is poor. The separation be...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 彭忠华周华邵雷鸣朱杰英
Owner HANGZHOU MINSHENG HEALTHCARE CO LTD
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