Fe4O3 surface modification method and preparation method of polystyrene magnetic microsphere

Abstract

The invention relates to a surface modification method of Fe3O4 and a method for preparing magnetic microspheres of polymerizing styrene, which belongs to the technical field of biology. The invention aims at providing the method for preparing the magnetic microspheres by modifying the surface of Fe3O4. The invention adopts an improved coprecipitation method to prepare monodisperse lipophilic Fe3O4 particles and then adopts the modified Fe3O4 to prepare the magnetic microspheres of polymerizing styrene, namely, the invention adopts a dispersion polymerization method to prepare superparamagnetic immune microspheres coated with high-molecular styrene polymer. The invention realizes that the superparamagnetic microspheres with even dispersion, controllable particle size, tight coating, strong magnetic property and high content of surface functional group are prepared.

Description

Technical field: [0001] The invention belongs to the field of biotechnology. Background technique: [0002] Magnetic microspheres are a new type of functional material developed in recent years. The magnetic balls contain a magnetic core. Therefore, under the action of an external magnetic field, the magnetic balls can be easily separated from the bottom liquid. It has the characteristics of polymer microspheres: such as A variety of reactive functional groups (-COOH, -NH2, -CHO, etc.) can be introduced on the surface of microspheres through chemical reactions such as copolymerization and surface modification, so that the magnetic balls can couple biological activities such as enzymes, cells, antibodies, nucleic acids, etc. Substances can be separated under a magnetic field, which makes the traditional expensive, time-consuming and labor-intensive immunoassay process simple, fast and low-cost. The special property of "directed movement in the presence of an external magneti...

AI Technical Summary

Problems solved by technology

[0004] Magnetic microspheres are a new technology that combines biology and chemical synthesis developed in the mid-1980s. It involves many disciplines such as inorganic chemistry, organic chemistry, material science, biomedicine and immunology, and requires multiple disciplines. At present, only a few countries in the world, such as France, Germany, the United States, and Japan, have mastered the technology of producing this kind of polymer magnetic material. At present, the application of this kind of magnetic material in various fields in our country relies on imports, which are expensive and difficult to popularize and apply.

In recent years, many domestic researchers have carried out research in this area and obtained patent rights in this field. However, after careful analysis and experiments, it was found that the magnetic microspheres synthesized by domestic inventions have some shortcomings: (1) serious agglomeration, poor dispersion solved

(2) The particle size distribution of magnetic microspheres is uneven, and it is difficult to prepare a uniform and stable microsphere suspension

(3) The polymer layer coating on the surface of the magnetic microspheres is not tight enough, the acid and alkali resistance is not good enough, and the specificity of the experiment will also be reduced.

(4) The content of functional groups on the surface of magnetic microspheres is too low to achieve the purpose of improving sensitivity. These deficiencies are not conducive to the application of magnetic microspheres in biomedicine and other fields

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