Preparation and use of swine foot-and-mouth disease recombinant immune composite peptide

Abstract

The invention relates to a method for constructing porcine fmd recombinant immune complex peptide and an application thereof, which belongs to the technical field of biological engineering. The immune multi-peptide adjuvant is fused with aphthovirus novel auxiliary T cell epiposition (I-S-I-S-E-I-K-G-V-I-V-H-K-I-E-T-I-L-F) and Bursin mimic peptide (T-P-N-L-K-H-G) by a flexible Linker. A fusion gene is inserted into an expression carrier to be converted into colon bacillus, gene engineering strains which express the porcine fmd recombinant immune complex peptide with high efficiency are obtained, and the fusion protein of the porcine fmd recombinant immune complex peptide is obtained after liquid culture and purification; the thioredoxin in the fusion protein is removed by enterokinase with His label on the N end, and the single porcine fmd recombinant immune complex peptide can be obtained after affinity chromatography purification with very high sample purity. The recombinant immune complex peptide can serve as the novel immune multi-peptide adjuvant of the foot-and-mouth disease vaccine. Animal immune experiments prove that the method has high safety but no toxicity and side effect, and can effectively enhance the immunity of organisms and cells and the immunity level of body fluid.

Description

technical field [0001] The invention relates to a preparation method and application of porcine foot-and-mouth disease recombinant immune complex peptide, and belongs to the technical field of genetic engineering production of immune adjuvants in the biotechnology pharmaceutical industry. Background technique [0002] Foot-and-mouth disease (FMD) is a severe infectious disease caused by foot-and-mouth disease virus (FMDV), which mainly infects artiodactyla animals. In addition to the direct economic loss caused by the death of animals, the stoppage of meat and milk production during the period of animal illness leads to the suspension of import and export trade of livestock products in the region and even the country, which also causes huge economic losses. Therefore, countries all over the world attach great importance to the prevention and control of this disease. [0003] FMDV infection requires the participation of B cells and T cells to produce neutralizing antibodies....

AI Technical Summary

Problems solved by technology

Since the natural BS can only be extracted from the chicken bursa tissue, the source is very limited, and the content of other impurities is high, so the purification is difficult, and its actual effect is also greatly affected

Chemically synthesized BS is expensive and not suitable for field promotion

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