Non-virus non-structural protein foot-and-mouth disease vaccine and preparation thereof

Abstract

The invention discloses a method for preparing a foot-and-mouth disease vaccine. The preparation method of the invention comprises the steps that monoclonal antibodies of non-structure protein of foot and mouth disease virus is used for removing non-structure protein of foot and mouth disease virus in a target object which is then prepared into the foot-and-mouth disease vaccine, wherein, the target object is a foot-and-mouth disease virus culture or a gene engineering expression product comprising the non-structure protein of foot and mouth disease virus and structure protein of foot and mouth disease virus. The reliability is greatly improved when infected animals are distinguished from immune animals in vaccine immunization animals using the foot-and-mouth disease vaccine of the invention; in addition, the method for preparing the foot-and-mouth disease vaccine of virus-free non-structure protein is simple, convenient and effective without extra expensive equipment and can be applied on a large scale. Therefore, the foot-and-mouth disease vaccine of the virus-free non-structure protein and the preparation method thereof have great significance in the quarantine of the foot-and-mouth disease vaccine and are about to play important roles in the prevention and control of the foot-and-mouth disease.

Description

technical field [0001] The invention relates to a foot-and-mouth disease vaccine without virus non-structural protein and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is a severe infectious disease that harms cloven-hoofed animals, especially pigs, cattle, and sheep, which are the main breeds of livestock, and can cause a decline in the productivity of livestock breeds and negative social impacts. Because of its high infectivity, the World Organization for Animal Health (FAO / OIE) lists it as the first class A infectious disease. Countries where the disease is endemic are forced to embargo international and regional trade, resulting in huge economic losses. In many countries in the world, especially in Africa, Asia and South America, the disease occurs frequently and the epidemic is serious. In addition, global epidemic outbreaks occur from time to time. [0003] The pathogen of foot-and-mouth disease is foot-and-mouth disease vi...

AI Technical Summary

Problems solved by technology

But some genetically engineered vaccines also use some non-structural protein genes of foot-and-mouth disease virus (Abrams C C, King A M Q, Belsham G J. Assembly of foot-and-mouth disease virus empty capsids synthesized by a vaccine virus expression system. J Gen Virol, 1995, 76:3089-3098.), there are these non-structural proteins in the vaccine, and the corresponding antibodies can also be produced after multiple immunization of animals, which also brings obstacles to distinguish infected animals from immunized animals

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